Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,310,436	T→C	D182G (GAC→GGC)	alsA ←	D‑allose ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,310,436	0	T	C	100.0%	248.8 / NA	67	D182G (GAC→GGC)	alsA	D‑allose ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/32);  total (35/32)

GCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGTCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTGATAGATAAATTCGCCACTTTCTCATCTAGATCAA  >  NC_000913/4310300‑4310571                                                                                                                                         |                                                                                                                                        gCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCa                                                                                                                                      >  2:249807/1‑140 (MQ=255) gCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCa                                                                                                                                      >  2:659571/1‑140 (MQ=255) gCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCa                                                                                                                                      >  2:698943/1‑140 (MQ=255) gCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGACGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCa                                                                                                                                      >  2:543257/1‑140 (MQ=255)  ccGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCAc                                                                                                                                     <  2:311329/140‑1 (MQ=255)  ccGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCAc                                                                                                                                     >  2:191378/1‑140 (MQ=255)  ccGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCAc                                                                                                                                     <  1:536400/140‑1 (MQ=255)  ccGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCAc                                                                                                                                     <  2:231332/140‑1 (MQ=255)   cGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGACGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCAcc                                                                                                                                    <  1:543257/140‑1 (MQ=255)        cAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCttt                                                                                                                               <  1:249807/140‑1 (MQ=255)         aaaCGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTa                                                                                                                              <  1:506594/140‑1 (MQ=255)             gctgctGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTgg                                                                                                                          >  2:652688/1‑140 (MQ=255)                  tgcCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGgtgagt                                                                                                                     <  1:86431/140‑1 (MQ=255)                  tgcCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGgtgagt                                                                                                                     <  1:221078/140‑1 (MQ=255)                     cGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGtgagtgag                                                                                                                  <  1:659571/140‑1 (MQ=255)                     cGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGtgagtgag                                                                                                                  <  1:698943/140‑1 (MQ=255)                          ttCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGt                                                                                                             <  2:189277/140‑1 (MQ=255)                           tcatcaCCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTg                                                                                                            >  2:173659/1‑140 (MQ=255)                              tcaCCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTTTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGt                                                                                                         <  2:321276/140‑1 (MQ=255)                                  cGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTC‑TTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGGGGGTTCATc                                                                                                    >  2:204604/1‑140 (MQ=255)                                     ataGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCa                                                                                                  <  1:757358/140‑1 (MQ=255)                                      taGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCat                                                                                                 >  2:771623/1‑140 (MQ=255)                                         cGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCatgat                                                                                              >  2:830183/1‑140 (MQ=255)                                           gTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCatgatga                                                                                            >  1:502091/1‑140 (MQ=255)                                           gTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCatgatga                                                                                            >  1:601693/1‑140 (MQ=255)                                            tCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCatgatgat                                                                                           >  1:153892/1‑140 (MQ=255)                                            tCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCatgatgat                                                                                           >  2:635688/1‑140 (MQ=255)                                            tCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCatgatgat                                                                                           <  2:685408/140‑1 (MQ=255)                                                            tttCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGc                                                                           >  1:569684/1‑140 (MQ=255)                                                                    aaCTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGt                                                                   >  1:67033/1‑140 (MQ=255)                                                                         aTGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTgg                                                              <  1:771623/140‑1 (MQ=255)                                                                            cGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCaa                                                           <  1:557209/140‑1 (MQ=255)                                                                                      aCGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCAt                                                 <  1:416096/140‑1 (MQ=255)                                                                                      aCGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCAt                                                 <  1:830183/140‑1 (MQ=255)                                                                                      aCGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGACAATTTCTAGCAt                                                 <  2:502091/140‑1 (MQ=255)                                                                                         aTGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGc                                                             >  1:486565/1‑125 (MQ=255)                                                                                         aTGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGc                                                             <  2:486565/125‑1 (MQ=255)                                                                                          tGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGc                                             >  1:328252/1‑140 (MQ=255)                                                                                           ggCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCGTCAGCGTTTTGGCAATTTCTAGCAt                                                 >  1:677704/1‑135 (MQ=255)                                                                                           ggCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCGTCAGCGTTTTGGCAATTTCTAGCAt                                                 <  2:677704/135‑1 (MQ=255)                                                                                           ggCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCt                                            >  2:29216/1‑140 (MQ=255)                                                                                           ggCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCt                                            >  1:603367/1‑140 (MQ=255)                                                                                           ggCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCt                                            >  2:186593/1‑140 (MQ=255)                                                                                           ggCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCt                                            >  1:670947/1‑140 (MQ=255)                                                                                           ggCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCt                                            >  2:600055/1‑140 (MQ=255)                                                                                           ggCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTGTGGCAATTTCTAGCATCTGCt                                            >  2:754323/1‑140 (MQ=255)                                                                                            gCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCtt                                           <  2:153892/140‑1 (MQ=255)                                                                                             ccGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTtg                                          >  1:5274/1‑140 (MQ=255)                                                                                             ccGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTtg                                          >  2:264402/1‑140 (MQ=255)                                                                                                 aCCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGc                                      <  1:173659/140‑1 (MQ=255)                                                                                                  cccTCTTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCt                                     <  1:29216/140‑1 (MQ=255)                                                                                                      ctTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTgata                                 >  1:180205/1‑140 (MQ=255)                                                                                                      ctTTACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTgata                                 >  1:393531/1‑140 (MQ=255)                                                                                                       tttACGCAACTGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTgatag                                <  1:635688/140‑1 (MQ=255)                                                                                                                 tGATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTGATAGATAAATTCGc                      >  1:595232/1‑140 (MQ=255)                                                                                                                  gATTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTGATAGATAAATTCGcc                     <  1:191378/140‑1 (MQ=255)                                                                                                                   aTTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTGATAGATAAATTCGCCa                    >  1:412903/1‑140 (MQ=255)                                                                                                                   aTTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTGATAGATAAATTCGCCa                    <  1:617635/140‑1 (MQ=255)                                                                                                                   aTTCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTGATAGATAAATTCGCCa                    >  2:400965/1‑140 (MQ=255)                                                                                                                     tCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTGATAGATAAATTCGCCACt                  <  1:754323/140‑1 (MQ=255)                                                                                                                     tCATGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTGATAGATAAATTCGCCACt                  <  2:180205/140‑1 (MQ=255)                                                                                                                       aTGATCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTGATAGATAAATTCGCCACttt                <  1:264402/140‑1 (MQ=255)                                                                                                                          aTCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTGATAGATAAATTCGcc                     <  2:200574/132‑1 (MQ=255)                                                                                                                          aTCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTGATAGATAAATTCGcc                     >  1:200574/1‑132 (MQ=255)                                                                                                                          aTCAGAAACAGATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTGATAGATAAATTCGCCACTTtctc             >  1:756778/1‑140 (MQ=255)                                                                                                                                    gATAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTGATAGATAAATTCGCCACTTTCTCATCTAGATCa   <  1:400965/140‑1 (MQ=255)                                                                                                                                     aTAGCCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTGATAGATAAATTCGCCACTTTCTCATCTAGATCaa  >  1:290374/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GCCGCTGCAAACGCTGCTGCCGTCTTTCATCACCGTATAGCGGTCGCAAATACGGCGAATTTCCGCCAACTTATGCGAGATATAGACGATGGCCGTACCCTCTTTACGCAACTGATTCATGATCAGAAACAGATAGTCCACCTCTTTATTGGTGAGTGAGGAGGTGGGTTCATCCATGATGATGACTTTGGCATCGAGCATCAGCGTTTTGGCAATTTCTAGCATCTGCTTGTGGCTGATAGATAAATTCGCCACTTTCTCATCTAGATCAA  >  NC_000913/4310300‑4310571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑