Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,400,982	C→T	T104I (ACC→ATC)	hflX →	ribosome rescue factor HflX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,400,982	0	C	T	100.0%	141.3 / NA	41	T104I (ACC→ATC)	hflX	ribosome rescue factor HflX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/20);  total (21/20)

GTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGG  >  NC_000913/4400847‑4401118                                                                                                                                        |                                                                                                                                         gTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGc                                                                                                                                      >  1:657421/1‑140 (MQ=255)   gAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCt                                                                                                                                    <  2:155751/140‑1 (MQ=255)           aGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAg                                                                                                                            >  1:527460/1‑140 (MQ=255)            gCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGa                                                                                                                           <  2:49243/140‑1 (MQ=255)                ttGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATAtt                                                                                                                       >  1:669415/1‑140 (MQ=255)                 tGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATAttt                                                                                                                      >  2:663958/1‑140 (MQ=255)                 tGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATAttt                                                                                                                      >  1:616648/1‑140 (MQ=255)                 tGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATAttt                                                                                                                      >  1:590988/1‑140 (MQ=255)                         cGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCaa                                                                                                              >  2:622413/1‑140 (MQ=255)                              gCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTgc                                                                                                         >  1:539349/1‑140 (MQ=255)                              gCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTgc                                                                                                         <  1:663958/140‑1 (MQ=255)                              gCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTgc                                                                                                         >  1:435540/1‑140 (MQ=255)                                   cAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTAGCCCAACGTGCGCGTa                                                                                                    <  2:355030/140‑1 (MQ=255)                                     aaGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTAcc                                                                                                  <  1:676871/140‑1 (MQ=255)                                         gACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATg                                                                                              <  1:108386/140‑1 (MQ=255)                                          aCGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTgc                                                                                                         >  1:390759/1‑128 (MQ=255)                                          aCGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTgc                                                                                                         <  2:390759/128‑1 (MQ=255)                                          aCGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGa                                                                                             <  1:14334/140‑1 (MQ=255)                                              gTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTCCCCATGAGGGt                                                                                         <  2:557286/140‑1 (MQ=255)                                              gTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGt                                                                                         <  2:590988/140‑1 (MQ=255)                                              gTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGt                                                                                         <  2:550613/140‑1 (MQ=255)                                              gTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGt                                                                                         <  2:527460/140‑1 (MQ=255)                                                                 tGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTgg                                                                      >  2:634793/1‑140 (MQ=255)                                                                       tGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCGGGTTGAGCTGGCGCAGc                                                                >  2:474195/1‑140 (MQ=255)                                                                       tGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGc                                                                >  2:421018/1‑140 (MQ=255)                                                                         cccTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTg                                                              <  2:616648/140‑1 (MQ=255)                                                                           cTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTgcg                                                            <  1:421018/140‑1 (MQ=255)                                                                                   ggCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTgg                                                    >  2:335991/1‑140 (MQ=255)                                                                                   ggCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTgg                                                    >  1:661169/1‑140 (MQ=255)                                                                                   ggCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTgg                                                    >  2:96570/1‑140 (MQ=255)                                                                                      gcAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTa                                                 <  2:669415/140‑1 (MQ=255)                                                                                               tAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGc                                                   <  2:313284/129‑1 (MQ=255)                                                                                               tAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGc                                                   >  1:313284/1‑129 (MQ=255)                                                                                                           tttGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGa                            >  2:676558/1‑140 (MQ=255)                                                                                                           tttGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGa                            >  2:301869/1‑140 (MQ=255)                                                                                                           tttGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGa                            <  1:676558/140‑1 (MQ=255)                                                                                                           tttGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGa                            >  1:139510/1‑140 (MQ=255)                                                                                                           tttGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGACATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGa                            >  1:446034/1‑140 (MQ=255)                                                                                                            ttGTGCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGAc                           <  2:657421/140‑1 (MQ=255)                                                                                                               tgCGAGTGTCGTGTTATCGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCa                        <  1:335991/140‑1 (MQ=255)                                                                                                                                cGACCGCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAg       <  2:363431/140‑1 (MQ=255)                                                                                                                                     gCATCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCggg  <  2:139510/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GTGAAGGTAAAGCAGTTGAAATTGCGGAAGCTGTCAAAGCGACGGGTGCTTCGGTCGTTCTTTTTGACCATGCCCTGAGCCCGGCGCAAGAGCGTAACCTGGAGCGTTTGTGCGAGTGTCGTGTTATCGACCGCACCGGCCTTATTTTAGATATTTTCGCCCAACGTGCGCGTACCCATGAGGGTAAGTTGCAGGTTGAGCTGGCGCAGCTGCGCCATCTGGCTACGCGCCTGGTGCGTGGCTGGACCCACCTTGAAAGACAGAAAGGCGGG  >  NC_000913/4400847‑4401118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑