Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	700,166	T→C	N54S (AAC→AGC)	umpH ←	UMP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	700,166	0	T	C	100.0%	140.1 / NA	38	N54S (AAC→AGC)	umpH	UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/21);  total (17/21)

CAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCAC  >  NC_000913/700030‑700290                                                                                                                                         |                                                                                                                             cAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCg                                                                                                                           <  1:142429/140‑1 (MQ=255)       aTGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGAt                                                                                                                     <  2:103413/140‑1 (MQ=255)         gAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCt                                                                                                                   >  2:310250/1‑140 (MQ=255)             cAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGcc                                                                                                               <  2:42570/140‑1 (MQ=255)              aGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGccc                                                                                                              <  1:594760/140‑1 (MQ=255)                    ccTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTg                                                                                                        <  1:49896/140‑1 (MQ=255)                     cTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGc                                                                                                       >  1:235091/1‑140 (MQ=255)                                         ttCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCaa                                                                                   >  2:826429/1‑140 (MQ=255)                                         ttCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCaa                                                                                   >  1:597174/1‑140 (MQ=255)                                                   ccTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGc                                                                         >  1:94212/1‑140 (MQ=255)                                                   ccTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGc                                                                         >  1:68620/1‑140 (MQ=255)                                                    cTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCa                                                                        <  1:45118/140‑1 (MQ=255)                                                    cTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCa                                                                        <  1:341657/140‑1 (MQ=255)                                                    cTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCa                                                                        <  1:310250/140‑1 (MQ=255)                                                           cGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCGACACCAGCGGCAGGCCttt                                                                 <  1:167745/140‑1 (MQ=255)                                                           cGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCttt                                                                 <  2:457971/140‑1 (MQ=255)                                                           cGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCttt                                                                 <  2:68620/140‑1 (MQ=255)                                                           cGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCttt                                                                 <  2:735047/140‑1 (MQ=255)                                                           cGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCttt                                                                 <  2:94212/140‑1 (MQ=255)                                                                      ggCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATcc                                                      >  1:75606/1‑140 (MQ=255)                                                                         aGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGt                                                   <  1:761557/140‑1 (MQ=255)                                                                            cGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCa                                                >  2:754166/1‑140 (MQ=255)                                                                                  cGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAAtt                                          <  1:185497/140‑1 (MQ=255)                                                                                  cGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAAtt                                          >  2:252846/1‑140 (MQ=255)                                                                                     agagGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCg                                       <  2:75606/140‑1 (MQ=255)                                                                                      gagGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGc                                      <  2:597174/140‑1 (MQ=255)                                                                                       agGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCCAAAATTCCGCt                                     >  2:510787/1‑140 (MQ=255)                                                                                       agGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCt                                     <  1:113233/140‑1 (MQ=255)                                                                                          tataAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCa                                  <  1:754166/140‑1 (MQ=255)                                                                                            taAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCAcc                                >  1:335774/1‑140 (MQ=255)                                                                                            taAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCAcc                                >  2:689586/1‑140 (MQ=255)                                                                                                   cGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACg                         >  1:414133/1‑140 (MQ=255)                                                                                                   cGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACg                         >  1:196629/1‑140 (MQ=255)                                                                                                      tGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCAcc                                >  1:610477/1‑130 (MQ=255)                                                                                                      tGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCAcc                                <  2:610477/130‑1 (MQ=255)                                                                                                      tGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCg                      >  2:157916/1‑140 (MQ=255)                                                                                                                          gCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCAc  <  1:510787/140‑1 (MQ=255)                                                                                                                          gCGGTGGCAAAGCGGCTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCAc  >  2:151106/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                             CAGTTCATGAATCAGTGCGCCTTCGCCCACCACATACGCTTTCTTGCCTTCCTGGCGACGCAGGAAATCGGCAGTCGCCATCGCAGAGGTATAAAACACGCTGTCAGGTACATCGACACCTGCGGTGGCAAAGCGGTTCGCCAGATCTTGCCCAGTCTGCGAAGGATAGTTGGTCAGCAACACCAGCGGCAGGCCTTTATCCATAATCCCGTGCAAAAATTCCGCTGCACCCGGTACGGCGACGTTATCGTGCATCAGCAC  >  NC_000913/700030‑700290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑