Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,409,574	C→T	L100L (CTC→CTT)	rlmB →	23S rRNA 2'‑O‑ribose G2251 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,409,574	0	C	T	100.0%	92.7 / NA	27	L100L (CTC→CTT)	rlmB	23S rRNA 2'‑O‑ribose G2251 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/13);  total (14/13)

CAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGC  >  NC_000913/4409440‑4409706                                                                                                                                       |                                                                                                                                     cAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACgg                                                                                                                                 <  1:763465/140‑1 (MQ=255)         cgacgaGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCg                                                                                                                         <  1:502414/140‑1 (MQ=255)           acgaGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGAt                                                                                                                       >  1:27664/1‑140 (MQ=255)            cgaGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATc                                                                                                                      <  2:650186/140‑1 (MQ=255)               gaAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGc                                                                                                                   >  2:606136/1‑140 (MQ=255)                           tGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGgcg                                                                                                       >  1:392765/1‑140 (MQ=255)                           tGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGgcg                                                                                                       <  1:441740/140‑1 (MQ=255)                           tGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGgcg                                                                                                       <  1:830190/140‑1 (MQ=255)                                       gggCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTa                                                                                           >  2:47445/1‑140 (MQ=255)                                            ttATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCg                                                                                      >  2:678245/1‑140 (MQ=255)                                                  cccGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGAcgcc                                                                                >  1:725508/1‑140 (MQ=255)                                                    cgcgTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGAcgccgc                                                                              <  2:725508/140‑1 (MQ=255)                                                    cgcgTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGAcgccgc                                                                              <  2:392765/140‑1 (MQ=255)                                                                              ggAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGa                                                    >  1:127648/1‑140 (MQ=255)                                                                              ggAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGa                                                    >  1:213817/1‑140 (MQ=255)                                                                                   aCGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGAt                                               >  2:357109/1‑140 (MQ=255)                                                                                            cGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGca                                      <  2:393147/140‑1 (MQ=255)                                                                                            cGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGca                                      <  2:420361/140‑1 (MQ=255)                                                                                            cGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGca                                      <  1:606136/140‑1 (MQ=255)                                                                                                 cTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCt                                 <  1:678245/140‑1 (MQ=255)                                                                                                     tcgcttcgctCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAAc                             >  2:459837/1‑140 (MQ=255)                                                                                                              tCGATCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCg                    >  1:789733/1‑140 (MQ=255)                                                                                                                  tCAACCGTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGaaaa                <  2:702500/140‑1 (MQ=255)                                                                                                                        gTTCCTGCTGATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAg          >  1:170138/1‑140 (MQ=255)                                                                                                                              gctgATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTgcg    >  2:130925/1‑140 (MQ=255)                                                                                                                              gctgATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGAGCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTgcg    >  2:768565/1‑140 (MQ=255)                                                                                                                                tgATCCTTGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTgcggc  <  2:674629/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     CAATATCTCGACGAGAAAAGCGACGGTGCCGTGCATCAGGGCATTATCGCCCGCGTGAAGCCAGGACGTCAGTATCAGGAAAACGATCTGCCGGATCTGATCGCTTCGCTCGATCAACCGTTCCTGCTGATCCTCGACGGCGTAACCGATCCGCACAACCTCGGCGCGTGCCTGCGTAGTGCGGACGCCGCAGGCGTTCATGCGGTGATTGTGCCGAAAGATCGCTCCGCACAGCTCAACGCTACGGCGAAAAAAGTAGCCTGCGGC  >  NC_000913/4409440‑4409706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑