Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,419,389	A→G	G79G (GGT→GGC)	ulaG ←	L‑ascorbate‑6‑phosphate lactonase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,419,389	0	A	G	100.0%	217.9 / NA	60	G79G (GGT→GGC)	ulaG	L‑ascorbate‑6‑phosphate lactonase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/28);  total (32/28)

GATATGATCGTTGTGATCGTGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAG  >  NC_000913/4419257‑4419525                                                                                                                                     |                                                                                                                                         gATATGATCGTTGTGATCGTGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGtt                                                                                                                                   <  1:446421/140‑1 (MQ=255)  atatGATCGTTGTGATCGTGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGttt                                                                                                                                  >  1:197871/1‑140 (MQ=255)      gATCGTTGTGATCGTGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCAtt                                                                                                                              >  2:153054/1‑140 (MQ=255)           ttGTGATCGTGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACgg                                                                                                                         <  1:10256/140‑1 (MQ=255)            tgtgATCGTGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACggg                                                                                                                        >  2:13402/1‑140 (MQ=255)             gtgATCGTGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGt                                                                                                                       >  2:796536/1‑140 (MQ=255)             gtgATCGTGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGt                                                                                                                       <  1:715351/140‑1 (MQ=255)               gATCGTGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTa                                                                                                                     >  2:292262/1‑140 (MQ=255)                  cGTGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCg                                                                                                                  >  2:234018/1‑140 (MQ=255)                    tGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACgg                                                                                                                         <  2:412734/131‑1 (MQ=255)                    tGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACgg                                                                                                                         >  1:412734/1‑131 (MQ=255)                    tGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTg                                                                                                                <  1:92839/140‑1 (MQ=255)                     gAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGa                                                                                                               <  1:13402/140‑1 (MQ=255)                     gAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGa                                                                                                               <  1:153054/140‑1 (MQ=255)                        tCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACtt                                                                                                            >  2:9584/1‑140 (MQ=255)                          gCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACtttg                                                                                                          >  2:719782/1‑140 (MQ=255)                               tACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACtttg                                                                                                          >  2:750322/1‑135 (MQ=255)                               tACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACtttg                                                                                                          <  1:750322/135‑1 (MQ=255)                               tACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGc                                                                                                     >  1:145660/1‑140 (MQ=255)                                aCCGCGTCGATCTGGCGAATCGCAAACGAATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGcc                                                                                                    <  2:480349/140‑1 (MQ=255)                                   gcgTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACt                                                                                                             >  2:257711/1‑128 (MQ=255)                                   gcgTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACt                                                                                                             <  1:257711/128‑1 (MQ=255)                                    cgTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTg                                                                                                >  1:436401/1‑140 (MQ=255)                                    cgTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTg                                                                                                >  2:685179/1‑140 (MQ=255)                                    cgTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTg                                                                                                >  1:592675/1‑140 (MQ=255)                                    cgTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTg                                                                                                >  2:269747/1‑140 (MQ=255)                                    cgTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTg                                                                                                >  2:25678/1‑140 (MQ=255)                                    cgTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTg                                                                                                >  2:774105/1‑140 (MQ=255)                                     gTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGc                                                                                               >  2:573035/1‑140 (MQ=255)                                      gcGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGcc                                                                                              >  2:88924/2‑140 (MQ=255)                                       cGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCg                                                                                             <  1:88924/140‑1 (MQ=255)                                       cGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCg                                                                                             <  2:197871/140‑1 (MQ=255)                                       cGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCg                                                                                             <  1:358432/140‑1 (MQ=255)                                         aTCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCa                                                                                           >  2:430674/1‑140 (MQ=255)                                         aTCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCa                                                                                           >  2:324031/1‑140 (MQ=255)                                          tCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCAc                                                                                          <  1:719782/140‑1 (MQ=255)                                                      cAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACg                                                                              <  2:145660/140‑1 (MQ=255)                                                        aaCGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCa                                                                            <  1:133847/140‑1 (MQ=255)                                                        aaCGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCa                                                                            <  1:796536/140‑1 (MQ=255)                                                             aTCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGt                                                                       <  1:292262/140‑1 (MQ=255)                                                                             gtggtACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTc                                                          <  1:220438/137‑1 (MQ=255)                                                                             gtggtACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTc                                                          >  2:220438/1‑137 (MQ=255)                                                                             gtggtACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGa                                                       >  2:320079/1‑140 (MQ=255)                                                                                 tACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTc                                                   <  1:573035/140‑1 (MQ=255)                                                                                  aCGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCa                                                  >  1:187543/1‑140 (MQ=255)                                                                                   cGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCaa                                                 <  2:592675/140‑1 (MQ=255)                                                                                                  aGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCa                                  >  2:542405/1‑140 (MQ=255)                                                                                                  aGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCa                                  >  1:454876/1‑140 (MQ=255)                                                                                                  aGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCa                                  <  1:376152/140‑1 (MQ=255)                                                                                                  aGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCa                                  >  2:172402/1‑140 (MQ=255)                                                                                                    ttttttCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                <  1:774105/140‑1 (MQ=255)                                                                                                    ttttttCACGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                <  2:765029/140‑1 (MQ=255)                                                                                                            cGCCAGCCATGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGccac                        <  1:685179/140‑1 (MQ=255)                                                                                                              ccagccaTGCGCTGCA‑CTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                >  2:224175/1‑129 (MQ=255)                                                                                                              ccagccaTGCGCTGCA‑CTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCagc                                <  1:224175/129‑1 (MQ=255)                                                                                                                  ccaTGCGCTGCATCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATc                  >  1:118436/1‑140 (MQ=255)                                                                                                                              tCTGGTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCa      >  1:814704/1‑140 (MQ=255)                                                                                                                                 ggTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGa   >  1:323869/1‑140 (MQ=255)                                                                                                                                 ggTGGCCCTGTTTCATTAACGGGATACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGa   >  2:767538/1‑140 (MQ=255)                                                                                                                                  gTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAg  <  2:118436/140‑1 (MQ=255)                                                                                                                                  gTGGCCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAg  <  1:542405/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         GATATGATCGTTGTGATCGTGAGTCGCCAGTACCGCGTCGATCTGGCGAATCGCAAACGGATCAAGAACAAACGGGGTGGTACGCAGGTTTGGCTGCAGTTTTTTCACGCCAGCCATGCGCTGCATCTGGTGACCCTGTTTCATTAACGGGTTACCGTGACTTTGTTTGCCAGTGCCGCACCAGAAATCAACGCAAACGTTGGTGCCACCTTCCGATTTCAACCAGATCCCGGTGCAGCCAAGCCACCACATCGCAAATGTGCCAGGAG  >  NC_000913/4419257‑4419525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑