Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,473,559	T→C	G165G (GGA→GGG)	bdcA ←	c‑di‑GMP‑binding biofilm dispersal mediator protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,473,559	0	T	C	100.0%	149.3 / NA	41	G165G (GGA→GGG)	bdcA	c‑di‑GMP‑binding biofilm dispersal mediator protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/23);  total (18/23)

ACCTCTTCCGGTTGCCCATGTCTTTTGATAGCCATCAAACTATGCAACATATCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGTCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCAT  >  NC_000913/4473428‑4473693                                                                                                                                    |                                                                                                                                        aCCTCTTCCGGTTGCCCATGTCTTTTGATAGCCATCAAACTATGCAACATATCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑c                                                                                                                                <  1:530657/140‑1 (MQ=255)     ctTCCGGTTGCCCATGTCTTTTGATAGCCATCAAACTATGCAACATATCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACgg                                                                                                                            >  1:373829/1‑140 (MQ=255)     ctTCCGGTTGCCCATGTCTTTTGATAGCCATCAAACTATGCAACATATCGCGCATTGGCCCGTTGGCGGGATTAGCGTAGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACgg                                                                                                                            >  1:533803/1‑140 (MQ=255)                ccATGTCTTTTGATAGCCATCAAACTATGCAACATATCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCcgcg                                                                                                                 >  1:807386/1‑140 (MQ=255)                  aTGTCTTTTGATAGCCATCAAACTATGCAACATATCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCcgcgcg                                                                                                               <  1:625052/140‑1 (MQ=255)                    gTCTTTTGATAGCCATCAAACTATGCAACATATCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGcc                                                                                                             <  2:578743/140‑1 (MQ=255)                    gTCTTTTGATAGCCATCAAACTATGCAACATATCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGcc                                                                                                             <  1:659113/140‑1 (MQ=255)                       ttttGATAGCCATCAAACTATGCAACATATCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATg                                                                                                          <  2:133375/140‑1 (MQ=255)                       ttttGATAGCCATCAAACTATGCAACATATCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATg                                                                                                          <  1:315006/140‑1 (MQ=255)                       ttttGATAGCCATCAAACTATGCAACATATCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGACCCAAAAT‑CACGGGCCAGCCCGCGCGCCATg                                                                                                          <  1:279745/140‑1 (MQ=255)                            aTAGCCATCAAACTATGCAACATATCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTg                                                                                                     <  1:442716/140‑1 (MQ=255)                             tAGCCATCAAACTATGCAACATATCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGGAGACGGTAATTGAGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCCCGCCATGCCTTGc                                                                                                    >  2:139804/1‑140 (MQ=255)                              aGCCATCAAACTATGCAACATATCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCa                                                                                                   >  2:410631/1‑140 (MQ=255)                                 cATCAAACTATGCAACATATCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAggg                                                                                                >  1:436752/1‑140 (MQ=255)                                   tCAAACTATGCAACATATCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCa                                                                                              >  2:62806/1‑140 (MQ=255)                                                cATATCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTggcgg                                                                                 <  2:795309/140‑1 (MQ=255)                                                 atatCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTggcggc                                                                                <  1:410631/140‑1 (MQ=255)                                                 atatCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTggcggc                                                                                <  2:155281/140‑1 (MQ=255)                                                     cgcgCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATaa                                                                            <  2:679134/140‑1 (MQ=255)                                                      gcgcATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAag                                                                           <  2:579888/140‑1 (MQ=255)                                                          aTTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAagcagc                                                                       >  2:181953/1‑140 (MQ=255)                                                           ttGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGcc                                                                      >  1:464538/1‑140 (MQ=255)                                                           ttGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGcc                                                                      <  1:96114/140‑1 (MQ=255)                                                            tGGCCCGTTGGCGGTATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGTCa                                                                     <  2:795331/140‑1 (MQ=255)                                                            tGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCa                                                                     >  2:80089/1‑140 (MQ=255)                                                            tGGCCCGTTGGCGGGATTAGCGTAGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCa                                                                     <  2:533803/140‑1 (MQ=255)                                                              gCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATg                                                                   >  1:58838/1‑140 (MQ=255)                                                               cccGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGc                                                                  <  1:76618/140‑1 (MQ=255)                                                                     ggCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCaa                                                            <  2:373829/140‑1 (MQ=255)                                                                      gCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAAc                                                           <  1:62806/140‑1 (MQ=255)                                                                                     gTATAAATGGGCCCGGCCTGGACGACGTTAATGGTCATCCCGCGCGG‑CCAAAATGCACGGGCCGGCCCGCGCGCCATGCCTTGCAGGGCATGTTTGCTGGCGGCATAAGCGGCCATGACTGCAACAGGCATACGATCGcg                                            <  2:625340/140‑2 (MQ=255)                                                                                                cccTGGCTGGACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGc                                 >  1:297378/1‑140 (MQ=255)                                                                                                   tggctggACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCga                              <  1:63457/140‑1 (MQ=255)                                                                                                    ggctggACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCgat                             >  1:45122/1‑140 (MQ=255)                                                                                                    ggctggACGACGTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCgat                             >  1:180983/1‑140 (MQ=255)                                                                                                          acgacgTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTaa                       >  2:115272/1‑140 (MQ=255)                                                                                                           cgacgTTAATGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAg                      <  2:464538/140‑1 (MQ=255)                                                                                                                  aaTGGTGATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGcgcc               <  2:297378/140‑1 (MQ=255)                                                                                                                        gATCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCtt         <  1:310555/140‑1 (MQ=255)                                                                                                                         aTCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTc        >  2:291428/1‑140 (MQ=255)                                                                                                                         aTCCCACGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTc        >  1:81078/1‑140 (MQ=255)                                                                                                                              aCGCGGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCa   >  1:445084/1‑140 (MQ=255)                                                                                                                               cgcgGCCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCAt  >  1:673763/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        ACCTCTTCCGGTTGCCCATGTCTTTTGATAGCCATCAAACTATGCAACATATCGCGCATTGGCCCGTTGGCGGGATTAGCGTCGGTATCAATTGGCCCTGGCTGGACGACGTTAATGGTGATCCCACGCGGTCCAAAAT‑CACGGGCCAGCCCGCGCGCCATGCCTTGCAGGGCAGATTTGCTGGCGGCATAAGCAGCCATGCCTGCAACAGGCATACGATCGCCATTCACGGAGCCGATGATTAAGATGCGCCCGCCTTCGGGCAT  >  NC_000913/4473428‑4473693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑