Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	743,909	C→T	A106V (GCG→GTG)	pxpB →	5‑oxoprolinase component B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	743,909	0	C	T	100.0%	153.5 / NA	47	A106V (GCG→GTG)	pxpB	5‑oxoprolinase component B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/24);  total (23/24)

GAGTCGCTGGCGCTGGATGCCATAGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGC  >  NC_000913/743773‑744043                                                                                                                                         |                                                                                                                                       gAGTCGCTGGCGCTGGATGCCATAGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCgtggc                                                                                                                                     <  1:610634/140‑1 (MQ=255) gAGTCGCTGGCGCTGGATGCCATAGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTGTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCgtggc                                                                                                                                     <  1:239834/140‑1 (MQ=255)  aGTCGCTGGCGCTGGATGCCATAGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCgtggcg                                                                                                                                    >  1:300211/1‑140 (MQ=255)       ctggcgctggATGCCATAGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTg                                                                                                                               >  2:830143/1‑140 (MQ=255)       ctggcgctggATGCCATAGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTg                                                                                                                               >  2:752182/1‑140 (MQ=255)       ctggcgctggATGCCATAGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTg                                                                                                                               >  1:519995/1‑140 (MQ=255)             ctggATGCCATAGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGtt                                                                                                                         <  1:277073/140‑1 (MQ=255)                            cGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGgt                                                                                                          <  1:752182/140‑1 (MQ=255)                            cGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGgt                                                                                                          >  1:92353/1‑140 (MQ=255)                            cGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGgt                                                                                                          >  1:366128/1‑140 (MQ=255)                              tttGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAg                                                                                                                      <  2:83379/126‑1 (MQ=255)                              tttGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAg                                                                                                                      >  1:83379/1‑126 (MQ=255)                               ttGGAACGCTGGGGGGATGTGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGG‑TTACGGTGGTGCAGGCGGGCCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGGTGAGGGAAAAACAGgttgtt                                                                                                      >  2:163387/1‑140 (MQ=255)                               ttGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGCCTCGCTTTATTGAAATTCCGGCGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGGGGTCGTGGCGTATTGCGGGTTGAGCGAAAAACAGgctgt                                                                                                       >  1:390319/1‑140 (MQ=255)                                 gCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGttgttg                                                                                                     <  1:698604/140‑1 (MQ=255)                                        tggtggGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCa                                                                                              <  1:830143/140‑1 (MQ=255)                                          gtggGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCAct                                                                                            <  2:764890/140‑1 (MQ=255)                                          gtggGAGGAGAGCAAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCAct                                                                                            >  2:66986/1‑140 (MQ=255)                                                ggagAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCg                                                                                      <  2:524470/140‑1 (MQ=255)                                                 gagaGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCgg                                                                                     >  2:102266/1‑139 (MQ=255)                                                 gagaGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGt                                                                                     <  2:300211/140‑1 (MQ=255)                                                    agCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCa                                                                                              <  2:303822/128‑1 (MQ=255)                                                    agCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCa                                                                                              >  1:303822/1‑128 (MQ=255)                                                    agCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGa                                                                                  >  1:724055/1‑140 (MQ=255)                                                      cGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAAt                                                                                <  2:635451/140‑1 (MQ=255)                                                        aGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCActcct                                                                                         >  1:812763/1‑129 (MQ=255)                                                        aGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCActcct                                                                                         <  2:812763/129‑1 (MQ=255)                                                         ggCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACg                                                                             >  1:48982/1‑140 (MQ=255)                                                           cgcTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTg                                                                           >  1:107386/1‑140 (MQ=255)                                                              tGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTc                                                                        <  1:66986/140‑1 (MQ=255)                                                              tGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTc                                                                        <  1:274845/140‑1 (MQ=255)                                                                   ccGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGtt                                                                   >  2:246331/1‑140 (MQ=255)                                                                          ctcGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGt                                                            >  1:603274/1‑140 (MQ=255)                                                                           tcGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGtt                                                           >  2:82819/1‑140 (MQ=255)                                                                                     gAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCggg                                                 <  2:689269/140‑1 (MQ=255)                                                                                     gAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCggg                                                 <  2:519995/140‑1 (MQ=255)                                                                                            cGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCg                                          <  2:366128/140‑1 (MQ=255)                                                                                                       ggtggtGCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAg                               <  1:102266/140‑1 (MQ=255)                                                                                                         tggtgCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGtt                             >  2:213490/1‑140 (MQ=255)                                                                                                           gtgCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTg                           >  1:301817/1‑140 (MQ=255)                                                                                                            tgCAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGc                          >  2:570907/1‑140 (MQ=255)                                                                                                              cAGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCg                        >  2:707268/1‑140 (MQ=255)                                                                                                               tggCGGACCGGATTTGGCGGTGGTCGTGTCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCAGTATCTCGGGAGTTTGCCga                       <  2:390319/139‑2 (MQ=255)                                                                                                               aGGCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCgg                       <  2:92353/140‑1 (MQ=255)                                                                                                                 gCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGaa                     <  2:361049/140‑1 (MQ=255)                                                                                                                 gCGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGaa                     <  2:107386/140‑1 (MQ=255)                                                                                                                  cGGACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGaac                    <  2:721690/140‑1 (MQ=255)                                                                                                                   ggACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGcc                         <  1:785173/134‑1 (MQ=255)                                                                                                                   ggACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGcc                         >  2:785173/1‑134 (MQ=255)                                                                                                                   ggACCGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGaaca                   >  2:9739/1‑140 (MQ=255)                                                                                                                      ccGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGc                          <  2:144877/130‑1 (MQ=255)                                                                                                                      ccGGATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGc                          >  1:144877/1‑130 (MQ=255)                                                                                                                        ggATTTGGCGGTGGTCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTac              <  1:570907/140‑1 (MQ=255)                                                                                                                                    ggtCGTGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGCGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGgc  >  2:154609/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       GAGTCGCTGGCGCTGGATGCCATAGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGC  >  NC_000913/743773‑744043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑