Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	751,895	C→T	G92D (GGT→GAT)	ybgQ ←	putative fimbrial usher protein YbgQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	751,895	0	C	T	100.0%	146.8 / NA	41	G92D (GGT→GAT)	ybgQ	putative fimbrial usher protein YbgQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/16);  total (25/16)

CAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGA  >  NC_000913/751766‑752023                                                                                                                                  |                                                                                                                                 cagGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATc                                                                                                                        <  2:36785/140‑1 (MQ=255)    gCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGAc                                                                                                                     <  1:307748/140‑1 (MQ=255)     cTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGAcc                                                                                                                    >  1:348436/1‑140 (MQ=255)         cGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTg                                                                                                                <  1:683751/140‑1 (MQ=255)              tACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGAc                                                                                                                     >  2:475950/1‑130 (MQ=255)              tACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGAc                                                                                                                     <  1:475950/130‑1 (MQ=255)                      ttCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCa                                                                                                   >  2:65179/1‑140 (MQ=255)                      ttCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCa                                                                                                   <  1:396885/140‑1 (MQ=255)                      ttCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCa                                                                                                   >  2:783728/1‑140 (MQ=255)                         aGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATgg                                                                                                >  2:97730/1‑140 (MQ=255)                                 aTTGGCTTGATCGGAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGttt                                                                                        >  1:85627/1‑140 (MQ=255)                                     gCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTc                                                                                    >  1:748311/1‑140 (MQ=255)                                               aaTTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCtttttt                                                                          >  2:135052/1‑140 (MQ=255)                                               aaTTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCtttttt                                                                          >  2:493072/1‑140 (MQ=255)                                                 ttGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTg                                                                        <  2:766045/140‑1 (MQ=255)                                                    aaGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCa                                                                     >  1:698456/1‑140 (MQ=255)                                                         cATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTaa                                                                >  2:283747/1‑140 (MQ=255)                                                                 gTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGc                                                                       >  1:594305/1‑125 (MQ=255)                                                                 gTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGc                                                                       <  2:594305/125‑1 (MQ=255)                                                                  tCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGcc                                                       <  1:769365/140‑1 (MQ=255)                                                                   cGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATTGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGccc                                                      >  1:156698/1‑140 (MQ=255)                                                                     ggAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCAAt                                                    >  1:99255/1‑140 (MQ=255)                                                                                  aTACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATTGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTttgtt                                       <  2:156698/140‑1 (MQ=255)                                                                                   tACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTttgttg                                      <  2:748311/140‑1 (MQ=255)                                                                                        cGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTttgttg                                      <  2:299030/135‑1 (MQ=255)                                                                                        cGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTttgttg                                      >  1:299030/1‑135 (MQ=255)                                                                                         gATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTc                                >  1:769979/1‑140 (MQ=255)                                                                                          atctatctGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTttg                                         <  2:567523/130‑1 (MQ=255)                                                                                          atctatctGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTttg                                         >  1:567523/1‑130 (MQ=255)                                                                                          atctatctGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCa                               >  2:422702/1‑140 (MQ=255)                                                                                           tctatctGTGGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCAc                              >  2:823618/1‑140 (MQ=255)                                                                                                  gtgGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACg                       >  1:112822/1‑140 (MQ=255)                                                                                                   tgGCAAGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGc                      >  2:555364/1‑140 (MQ=255)                                                                                                       aaGGACTGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAAc                  <  2:348436/140‑1 (MQ=255)                                                                                                             tGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGc                      >  2:726534/1‑130 (MQ=255)                                                                                                             tGACGGATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCAATACTGATTTTGTTGTTATTCACCGCAACGc                      <  1:726534/130‑1 (MQ=255)                                                                                                                  gATATCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCAc       <  1:555364/140‑1 (MQ=255)                                                                                                                   atatCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCAcc      >  2:668862/1‑140 (MQ=255)                                                                                                                    tatCTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCg     >  2:463344/1‑140 (MQ=255)                                                                                                                       cTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGa  >  2:164255/1‑140 (MQ=255)                                                                                                                       cTGGTTTTAAATCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGa  <  1:164255/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                 CAGGCTTGCGGAATACTAATATTCAGTTGCTGATTGGCTTGATCGAAATTGAAGAGCATTTCAGGTCGGGAACTGAAATCAATACACCGATCTATCTGTGGCAAGGACTGACGGATATCTGGTTTTAAACCAAATTTATCGACCAGTGAATCATTGATGCATGGAATGGTTTTGTCACCCTTTTTTTGCCAGTTAATTTTTTGCCCATTACTGATTTTGTTGTTATTCACCGCAACGCTAACAAAATATTCACCGGGA  >  NC_000913/751766‑752023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑