Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	972,557	A→G	D269G (GAT→GGT)	ycbJ →	putative phosphotransferase YcbJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	972,557	0	A	G	100.0%	132.9 / NA	36	D269G (GAT→GGT)	ycbJ	putative phosphotransferase YcbJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/18);  total (18/18)

TGGGCACCGCGAGAATACGAACTGTTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGATGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGC  >  NC_000913/972424‑972689                                                                                                                                      |                                                                                                                                     tGGGCACCGCGAGAATACGAACTGTTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGt                                                                                                                                >  1:497769/1‑140 (MQ=255) tGGGCACCGCGAGAATACGAACTGTTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGACGTTCATTTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGt                                                                                                                                >  1:430595/1‑140 (MQ=255)      aCCGCGAGAATACGAACTGTTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTgcgc                                                                                                                           <  2:430595/140‑1 (MQ=255)        cgcgAGAATACGAACTGTTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCaa                                                                                                                         <  2:185908/140‑1 (MQ=255)        cgcgAGAATACGAACTGTTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCaa                                                                                                                         <  2:43864/140‑1 (MQ=255)        cgcgAGAATACGAACTGTTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCaa                                                                                                                         <  1:438669/140‑1 (MQ=255)        cgcgAGAATACGAACTGTTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCaa                                                                                                                         <  1:806208/140‑1 (MQ=255)        cgcgAGAATACGAACTGTTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCaa                                                                                                                         >  2:28516/1‑140 (MQ=255)        cgcgAGAATACGAACTGTTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCaa                                                                                                                         <  1:668584/140‑1 (MQ=255)                         ttCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACg                                                                                                        >  1:223721/1‑140 (MQ=255)                                   tGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCgg                                                                                              >  2:106534/1‑140 (MQ=255)                                   tGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCgg                                                                                              >  1:38065/1‑140 (MQ=255)                                     gATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGgcg                                                                                            <  2:428689/140‑1 (MQ=255)                                               tGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGAt                                                                                  >  1:528530/1‑140 (MQ=255)                                                         tttGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCaa                                                                        >  1:134991/1‑140 (MQ=255)                                                            gCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAAt                                                                     <  2:528530/140‑1 (MQ=255)                                                                        ccTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGt                                                         >  2:162969/1‑140 (MQ=255)                                                                            cAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGcc                                                           >  1:1117/1‑134 (MQ=255)                                                                            cAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGcc                                                           <  2:1117/134‑1 (MQ=255)                                                                            cAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCt                                                     >  1:353926/1‑140 (MQ=255)                                                                                 cgcgcCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTg                                                        >  1:607071/1‑132 (MQ=255)                                                                                 cgcgcCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTg                                                        <  2:607071/132‑1 (MQ=255)                                                                                 cgcgcCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCt                                                >  1:130677/1‑140 (MQ=255)                                                                                              gAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTa                                   >  2:339891/1‑140 (MQ=255)                                                                                              gAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTa                                   >  2:414207/1‑140 (MQ=255)                                                                                                gTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGc                                 <  2:130677/140‑1 (MQ=255)                                                                                                gTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGc                                 <  1:496397/140‑1 (MQ=255)                                                                                                gTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGc                                 <  1:414207/140‑1 (MQ=255)                                                                                                gTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGc                                 <  2:593425/140‑1 (MQ=255)                                                                                                 tCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGcc                                <  2:353926/140‑1 (MQ=255)                                                                                                  cGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCa                               <  1:696857/140‑1 (MQ=255)                                                                                                  cGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCa                               <  2:53376/140‑1 (MQ=255)                                                                                                   gTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCAc                              >  1:35058/1‑140 (MQ=255)                                                                                                            gcggcgTTGGTTGTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATa                     >  1:9302/1‑140 (MQ=255)                                                                                                                    ggttgTATGTGTTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAg             >  1:539536/1‑140 (MQ=255)                                                                                                                             tgtTATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATa    <  2:9302/140‑1 (MQ=255)                                                                                                                               ttATGGGGTGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGc  >  2:549967/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                     TGGGCACCGCGAGAATACGAACTGTTCCGACTAATGGATAATTCTCTGGCGGAAGATTTGCTCTGGAGTTACCTGCAACGCGCGCCAGTGGCGGAGTCGTTCATCTGGCGGCGTTGGTTGTATGTGTTATGGGATGAAGTTGCGCAACTGGTTAATACCGGACGATTTAGTCGGCGCAACTTCGATCTGGCATCAAAATCACTCTTGCCGTGGCTCGCCTGACGAACCTTTTAGCCACTGCCAGATACGGCCAAGCGTTTCATAGC  >  NC_000913/972424‑972689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑