| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,124,719 | C→T | R203H (CGC→CAC) | mdtH ← | multidrug efflux pump MdtH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,124,719 | 0 | C | T | 97.4% | 126.8 / ‑4.2 | 39 | R203H (CGC→CAC) | mdtH | multidrug efflux pump MdtH |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (20/18); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
TACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAG > NC_000913/1124581‑1124841 | tACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCAcgcg > 2:331472/1‑140 (MQ=255) cATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTggg > 2:333236/1‑140 (MQ=255) aTTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATg > 1:351405/1‑140 (MQ=255) tAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCt > 1:283291/1‑140 (MQ=255) cGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTcg > 1:509658/1‑140 (MQ=255) gagGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGGAACCCGCCAGCGTCAGAACATAGGAGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAAc > 2:320923/1‑140 (MQ=255) ggCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACggg > 2:451841/1‑140 (MQ=255) ggCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACgag > 2:561620/1‑138 (MQ=255) gcgcgcCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGgc < 2:673188/140‑1 (MQ=255) cgcCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGgcgtg > 1:465437/1‑140 (MQ=255) aCCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGttt < 2:416445/140‑1 (MQ=255) aTAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCa > 1:4958/1‑140 (MQ=255) aaTTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATg < 2:148093/140‑1 (MQ=255) agcatcagcatcACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTaa < 1:747794/140‑1 (MQ=255) agcatcagcatcACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTaa > 2:747794/1‑140 (MQ=255) agcatcagcatcACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTaa > 2:216464/1‑140 (MQ=255) cagcatcaCTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAAcc < 1:333236/140‑1 (MQ=255) cTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTg > 2:727947/1‑140 (MQ=255) tACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAAcg < 2:283291/140‑1 (MQ=255) cagcaagcATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAAcgcc < 2:532648/140‑1 (MQ=255) ccagcATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGca > 2:191548/1‑140 (MQ=255) ccagcATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGca < 2:351405/140‑1 (MQ=255) cagcATGTAGTAACTCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTGCGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGcac < 2:509658/140‑1 (MQ=255) agcATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCAc > 2:63099/1‑123 (MQ=255) agcATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCAc < 1:63099/123‑1 (MQ=255) agcATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGcaca > 1:417709/1‑140 (MQ=255) tGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGc < 2:167355/140‑1 (MQ=255) tGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGc < 1:732778/140‑1 (MQ=255) gtaACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACaa < 1:331472/140‑1 (MQ=255) cccGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAg > 1:167395/1‑140 (MQ=255) gCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAAc > 2:63445/1‑140 (MQ=255) ccAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACt > 2:412583/1‑140 (MQ=255) gCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGcg < 2:167395/140‑2 (MQ=255) tCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGTGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCt < 2:722324/140‑1 (MQ=255) tCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCt < 2:324348/140‑1 (MQ=255) tCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCt < 2:4958/140‑1 (MQ=255) cATAGGTGACAAAACGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGgcg < 2:78447/140‑1 (MQ=255) aaaaCGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGc > 2:346542/1‑140 (MQ=255) aaaaCGCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGc < 1:101022/140‑1 (MQ=255) gCTTGTCACGCATCACGTGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAg > 2:440298/1‑140 (MQ=255) | TACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAG > NC_000913/1124581‑1124841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |