Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,198,052	T→C	A62A (GCA→GCG)	ymfE ←	e14 prophage; uncharacterized protein YmfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,198,052	0	T	C	100.0%	320.7 / NA	85	A62A (GCA→GCG)	ymfE	e14 prophage; uncharacterized protein YmfE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/45);  total (40/45)

CGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGTGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTT  >  NC_000913/1197917‑1198188                                                                                                                                        |                                                                                                                                         cGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGccc                                                                                                                                      >  1:96253/1‑140 (MQ=255) cGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGCAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGccc                                                                                                                                      <  2:720818/140‑1 (MQ=255)  ggTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCa                                                                                                                                     <  2:665487/140‑1 (MQ=255)    ttttATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAAt                                                                                                                                   <  2:309461/140‑1 (MQ=255)            ttAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAg                                                                                                                           <  1:553746/140‑1 (MQ=255)            ttAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAg                                                                                                                           <  2:253077/140‑1 (MQ=255)              aGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGct                                                                                                                         >  2:520926/1‑140 (MQ=255)               gATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGctc                                                                                                                        <  2:96253/140‑1 (MQ=255)                aTGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGctct                                                                                                                       >  1:326646/1‑140 (MQ=255)                  gCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCa                                                                                                                     <  2:400757/140‑1 (MQ=255)                    ttATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATg                                                                                                                   >  2:395241/1‑140 (MQ=255)                    ttATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATg                                                                                                                   <  1:720571/140‑1 (MQ=255)                    ttATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATg                                                                                                                   <  1:473447/140‑1 (MQ=255)                     tATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGa                                                                                                                  <  2:326646/140‑1 (MQ=255)                     tATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGa                                                                                                                  <  1:642200/140‑1 (MQ=255)                      aTCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGAt                                                                                                                 >  2:3092/1‑140 (MQ=255)                      aTCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGAt                                                                                                                 >  2:83293/1‑140 (MQ=255)                      aTCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGAt                                                                                                                 >  1:137731/1‑140 (MQ=255)                      aTCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGAt                                                                                                                 >  1:541604/1‑140 (MQ=255)                      aTCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGAt                                                                                                                 >  1:438531/1‑140 (MQ=255)                            aaGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTaa                                                                                                           <  1:361373/140‑1 (MQ=255)                            aaGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTaa                                                                                                           <  2:240780/140‑1 (MQ=255)                            aaGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTaa                                                                                                           <  1:284813/140‑1 (MQ=255)                             aGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAAt                                                                                                          <  2:438531/140‑1 (MQ=255)                                tatCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAgg                                                                                                       <  1:83293/140‑1 (MQ=255)                                tatCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAgg                                                                                                       <  2:137731/140‑1 (MQ=255)                                tatCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAgg                                                                                                       <  2:244260/140‑1 (MQ=255)                                tatCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAgg                                                                                                       <  1:520926/140‑1 (MQ=255)                                tatCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAgg                                                                                                       <  1:441774/140‑1 (MQ=255)                                 atCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCa                                                                                                                     >  1:208582/1‑125 (MQ=255)                                 atCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCa                                                                                                                     <  2:208582/125‑1 (MQ=255)                                  tCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTg                                                                                                     <  1:177511/140‑1 (MQ=255)                                    gCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTggg                                                                                                   <  2:618335/140‑1 (MQ=255)                                    gCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTggg                                                                                                   <  1:401842/140‑1 (MQ=255)                                       cccGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACa                                                                                                <  2:78381/140‑1 (MQ=255)                                        ccGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACAt                                                                                               >  1:327195/1‑140 (MQ=255)                                         cGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATGTACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATa                                                                                              >  2:725778/1‑140 (MQ=255)                                         cGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATa                                                                                              >  1:454137/1‑140 (MQ=255)                                                     gCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTaa                                                                                  <  1:43236/140‑1 (MQ=255)                                                      cTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAAt                                                                                 <  2:565463/140‑1 (MQ=255)                                                        gCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGt                                                                               >  1:273791/1‑140 (MQ=255)                                                           ttGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATg                                                                                >  1:251086/1‑136 (MQ=255)                                                           ttGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATg                                                                                <  2:251086/136‑1 (MQ=255)                                                             gTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGttatta                                                                          <  1:725778/140‑1 (MQ=255)                                                              ttATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTaa                                                                         <  2:273791/140‑1 (MQ=255)                                                                      gTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATg                                                                  <  2:663922/139‑1 (MQ=255)                                                                      gTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATg                                                                  >  1:663922/1‑139 (MQ=255)                                                                      gTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGt                                                                 >  1:432256/1‑140 (MQ=255)                                                                      gTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGt                                                                 >  2:453460/1‑140 (MQ=255)                                                                      gTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGt                                                                 >  2:788968/1‑140 (MQ=255)                                                                      gTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGt                                                                 >  1:808146/1‑140 (MQ=255)                                                                        cTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAggg                                                                     >  1:402055/1‑134 (MQ=255)                                                                        cTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAggg                                                                     <  2:402055/134‑1 (MQ=255)                                                                        cTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTAt                                                               >  2:395672/1‑140 (MQ=255)                                                                        cTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTAt                                                               >  2:779782/1‑140 (MQ=255)                                                                        cTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTAt                                                               >  1:719032/1‑140 (MQ=255)                                                                           aCGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCg                                                            >  2:420186/1‑140 (MQ=255)                                                                            cGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGt                                                           >  2:76855/1‑140 (MQ=255)                                                                              cACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTaa                                                         <  2:800409/140‑1 (MQ=255)                                                                              cACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTaa                                                         <  1:395241/140‑1 (MQ=255)                                                                                ccGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAAt                                                       >  2:360319/1‑140 (MQ=255)                                                                                          aaTATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGt                                             <  2:454137/140‑1 (MQ=255)                                                                                           atatGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTa                                            <  1:779782/140‑1 (MQ=255)                                                                                           atatGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTa                                            <  2:477503/140‑1 (MQ=255)                                                                                           atatGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTa                                            <  1:630600/140‑1 (MQ=255)                                                                                           atatGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTa                                            <  1:360319/140‑1 (MQ=255)                                                                                             atGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATg                                          <  1:324770/140‑1 (MQ=255)                                                                                              tGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGc                                         >  1:805267/1‑140 (MQ=255)                                                                                               gCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCa                                        <  2:569544/140‑1 (MQ=255)                                                                                                           aaaGCACAAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGt                            >  2:140816/1‑140 (MQ=255)                                                                                                               cacaAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTAttt                        >  2:523282/1‑140 (MQ=255)                                                                                                               cacaAGGTAATATACCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTAttt                        >  2:577191/1‑140 (MQ=255)                                                                                                                            aCCAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCtt           >  1:47934/1‑140 (MQ=255)                                                                                                                              cAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTa         >  2:114004/1‑140 (MQ=255)                                                                                                                              cAAAAGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTa         >  2:770380/1‑140 (MQ=255)                                                                                                                               aaaaGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTAt        <  1:140816/140‑1 (MQ=255)                                                                                                                               aaaaGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTAt        >  1:116518/1‑140 (MQ=255)                                                                                                                               aaaaGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTAt        >  1:300564/1‑140 (MQ=255)                                                                                                                               aaaaGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTAt        >  2:27261/1‑140 (MQ=255)                                                                                                                                aaaGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATg       >  2:691374/1‑140 (MQ=255)                                                                                                                                aaaGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATg       >  2:520836/1‑140 (MQ=255)                                                                                                                                aaaGATAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATg       >  1:781778/1‑140 (MQ=255)                                                                                                                                    aTAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGt   <  1:523282/140‑1 (MQ=255)                                                                                                                                     tAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGtt  <  1:520836/140‑1 (MQ=255)                                                                                                                                     tAGCGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGtt  <  1:691374/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CGGTTTTATAATTAGATGCTTATCCCAAAGATATCGCACCCGAAGTAGTTTGGCTGCATTGTTATGTAGGTCTAACGCACCGCTAATTAAATATGCAAAAATCGCAAAAGCACAAGGTAATATACCAAAAGATAGTGCCCAATTAATAAAGCTCTCATGATCTTTAATAGGTGGGACATACTTGGTTGGTAATGTTATTAAAGGGATGTATTCGTAAATAACTAGAAGTATGCAATATATTAGCGTATTTAAGAAAGTTGCTTTATGAAGTT  >  NC_000913/1197917‑1198188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑