Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,372,756	G→A	G283D (GGT→GAT)	ycjN →	putative ABC transporter periplasmic binding protein YcjN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,372,756	0	G	A	100.0%	208.2 / NA	59	G283D (GGT→GAT)	ycjN	putative ABC transporter periplasmic binding protein YcjN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/30);  total (29/30)

AACACTATGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGGTTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGAGCAGGCA  >  NC_000913/1372626‑1372883                                                                                                                                   |                                                                                                                                aaCACTATGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCgtggt                                                                                                                        >  1:737084/1‑140 (MQ=255)       aTGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAAc                                                                                                                  >  2:189067/1‑140 (MQ=255)        tGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAAcc                                                                                                                 >  1:88996/1‑140 (MQ=255)         gCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCg                                                                                                                >  2:681299/1‑140 (MQ=255)           cGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAATGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCgag                                                                                                              >  2:413439/1‑140 (MQ=255)            gggTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCgaga                                                                                                             >  1:351228/1‑140 (MQ=255)              gTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGaaa                                                                                                           >  2:395709/1‑140 (MQ=255)                  tAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAAct                                                                                                       >  1:476445/1‑140 (MQ=255)                    aCGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAActct                                                                                                     <  2:790346/140‑1 (MQ=255)                    aCGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAActct                                                                                                     >  1:175366/1‑140 (MQ=255)                     cGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTg                                                                                                    <  2:284969/140‑1 (MQ=255)                     cGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTg                                                                                                    <  2:476445/140‑1 (MQ=255)                     cGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTg                                                                                                    <  1:758345/140‑1 (MQ=255)                     cGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTg                                                                                                    <  2:351228/140‑1 (MQ=255)                     cGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTg                                                                                                    <  1:378417/140‑1 (MQ=255)                       aCATCATGGAAGTGAAAGACGCCTTTATGACCGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCg                                                                                                  <  2:405644/140‑1 (MQ=255)                       aCATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCg                                                                                                  >  2:341249/1‑140 (MQ=255)                       aCATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCg                                                                                                  <  2:269433/140‑1 (MQ=255)                         atcatGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGt                                                                                                >  1:32690/1‑140 (MQ=255)                         atcatGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGt                                                                                                <  2:737084/140‑1 (MQ=255)                          tcatGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTc                                                                                               >  1:485521/1‑140 (MQ=255)                              ggAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAATGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACg                                                                                           <  1:413439/140‑1 (MQ=255)                              ggAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACg                                                                                           <  2:194227/140‑1 (MQ=255)                                     aaaGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGtt                                                                                    >  2:426334/1‑140 (MQ=255)                                         aCGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGAcc                                                                                <  2:32690/140‑1 (MQ=255)                                          cGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCt                                                                               >  2:173452/1‑140 (MQ=255)                                            ccTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCg                                                                             <  2:485521/140‑1 (MQ=255)                                            ccTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCg                                                                             >  2:347284/1‑140 (MQ=255)                                                       ggCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTg                                                                          <  1:521703/132‑1 (MQ=255)                                                       ggCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTg                                                                          >  2:521703/1‑132 (MQ=255)                                                       ggCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTAc                                                                  >  1:403016/1‑140 (MQ=255)                                                         cACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTAccg                                                                >  2:796229/1‑140 (MQ=255)                                                         cACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTAccg                                                                <  2:97978/140‑1 (MQ=255)                                                           ccGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGCGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTAccgcc                                                              <  2:774426/140‑1 (MQ=255)                                                                ccGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCa                                                         <  1:173452/140‑1 (MQ=255)                                                                                    cTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAag                                     <  1:395709/140‑1 (MQ=255)                                                                                       tatCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAagcag                                  >  2:23611/1‑140 (MQ=255)                                                                                        atCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAagcagc                                 >  1:105931/1‑140 (MQ=255)                                                                                        atCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAagcagc                                 >  2:466738/1‑140 (MQ=255)                                                                                         tCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAgcagca                                >  2:459252/1‑140 (MQ=255)                                                                                              ccGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGaaaa                           >  1:64604/1‑140 (MQ=255)                                                                                              ccGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGaaaa                           >  2:763201/1‑140 (MQ=255)                                                                                              ccGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGaaaa                           >  1:780805/1‑140 (MQ=255)                                                                                                    gtgATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGt                     <  1:763201/140‑1 (MQ=255)                                                                                                    gtgATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGt                     <  2:105931/140‑1 (MQ=255)                                                                                                    gtgATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGt                     <  2:88996/140‑1 (MQ=255)                                                                                                    gtgATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGt                     <  2:123149/140‑1 (MQ=255)                                                                                                     tgATTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTc                    >  2:795318/1‑140 (MQ=255)                                                                                                       aTTAAAGAAGGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCAc                  <  2:350018/140‑1 (MQ=255)                                                                                                            agaagGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTa             <  1:128951/140‑1 (MQ=255)                                                                                                             gaagGCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTAt            <  1:426334/140‑1 (MQ=255)                                                                                                                ggCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGa         >  1:415213/1‑140 (MQ=255)                                                                                                                ggCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGa         >  1:133749/1‑140 (MQ=255)                                                                                                                 gCGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGAg        <  1:341249/140‑1 (MQ=255)                                                                                                                  cGACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGAgc       <  1:285957/140‑1 (MQ=255)                                                                                                                   gACCCGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGAgca      <  2:756051/140‑1 (MQ=255)                                                                                                                      ccGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGt                     >  2:745549/1‑122 (MQ=255)                                                                                                                      ccGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGt                     <  1:745549/122‑1 (MQ=255)                                                                                                                       cGAAAAACGTCGATTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGAgcaggca  <  1:459252/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                AACACTATGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGGTTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGAGCAGGCA  >  NC_000913/1372626‑1372883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑