Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,485,167	A→G	T703A (ACC→GCC)	hrpA →	ATP‑dependent RNA helicase HrpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,485,167	0	A	G	100.0%	132.4 / NA	37	T703A (ACC→GCC)	hrpA	ATP‑dependent RNA helicase HrpA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/20);  total (17/20)

TATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGTGGGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCAAGGTCAACTACAGCCAGATCGATCCGGCGTTATGTCGTGAA  >  NC_000913/1485039‑1485301                                                                                                                                 |                                                                                                                                       tATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGTGGGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGaa                                                                                                                             <  1:271507/140‑1 (MQ=255)   ttCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGTGGGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAAcc                                                                                                                           >  1:354865/1‑140 (MQ=255)   ttCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGTGGGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAAcc                                                                                                                           >  1:51038/1‑140 (MQ=255)    tCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGTGGGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCg                                                                                                                          <  1:329063/140‑1 (MQ=255)             cGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGTGGGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGATGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGaa                                                                                                                 >  2:594263/1‑140 (MQ=255)                                 gCGGAACTGGTAGAAACCAGCCGCCTGTGGGGGCGCAGTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGAt                                                                                             >  2:732796/1‑140 (MQ=255)                                       cTGGTAGAAACCAGCCGCCTGTGGGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAAc                                                                                       >  1:730007/1‑140 (MQ=255)                                                aCCAGCCGCCTGTGGGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGt                                                                              >  1:515255/1‑140 (MQ=255)                                                  cAGCCGCCTGTGGGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCa                                                                            <  1:151235/140‑1 (MQ=255)                                                  cAGCCGCCTGTGGGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCa                                                                            <  2:590117/140‑1 (MQ=255)                                                  cAGCCGCCTGTGGGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGATGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGTAACGGAAAAAGTCa                                                                            <  1:594263/140‑1 (MQ=255)                                                     ccgccTGTGGGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTg                                                                         >  1:5155/1‑140 (MQ=255)                                                                   gcATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAGAGTCACTGTTTATGGTTTGCCg                                                           >  1:585892/1‑140 (MQ=255)                                                                   gcATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCg                                                           >  2:600024/1‑140 (MQ=255)                                                                   gcATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCg                                                           >  1:656437/1‑140 (MQ=255)                                                                      ttGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGAtt                                                        >  1:617247/1‑140 (MQ=255)                                                                             gcgTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCcg                                                 <  2:617247/140‑1 (MQ=255)                                                                                   cGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCa                                           <  1:186262/140‑1 (MQ=255)                                                                                   cGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCa                                           <  2:515255/140‑1 (MQ=255)                                                                                   cGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCa                                           <  2:51038/140‑1 (MQ=255)                                                                                   cGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCa                                           <  1:732796/140‑1 (MQ=255)                                                                                           aaTGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCAAGGTCAAc                                   <  1:511274/140‑1 (MQ=255)                                                                                               ggTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCAAGGTCAACTACa                               <  2:5155/140‑1 (MQ=255)                                                                                               ggTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCAAGGTCAACTACa                               <  2:585892/140‑1 (MQ=255)                                                                                                gTGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCAAGGTCAACTACAg                              >  2:277993/1‑140 (MQ=255)                                                                                                 tGGAGCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCAAGGTCAACTACAGc                             <  2:656437/140‑1 (MQ=255)                                                                                                     gCCAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCAAGGTCAACTACAGCCAga                         >  2:724812/1‑140 (MQ=255)                                                                                                      ccAGTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGACGCGCGCAAGGTCAACTACAGCCAgat                        <  2:745161/140‑1 (MQ=255)                                                                                                         gTTGCTCAGCATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCAAGGTCAACTACAGCCAgatcga                     >  1:649529/1‑140 (MQ=255)                                                                                                                aGCATTTGATTAAACGCGCGTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCAAGGTCAACTACAGCCAGATCGATCCGGCg              <  2:117004/140‑1 (MQ=255)                                                                                                                  cATTTGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCAAGGTCAACTACAGCCAGATCGATCCGGCGtt            <  1:724812/140‑1 (MQ=255)                                                                                                                    tttGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCAAGGTCAACTACAGCCAGATCGATCCGGCGTTAt          >  2:532517/1‑140 (MQ=255)                                                                                                                    tttGATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCAAGGTCAACTACAGCCAGATCGATCCGGCGTTAt          <  2:700742/140‑1 (MQ=255)                                                                                                                       gATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCAAGGTCAACTACAGCCAgatcgatc                   >  2:44360/1‑128 (MQ=255)                                                                                                                       gATTAAACGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCAAGGTCAACTACAGCCAgatcgatc                   <  1:44360/128‑1 (MQ=255)                                                                                                                           aaaCGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCAAGGTCAACTACAGCCAGATCGATCCGGCGTTATGTCGTGa   <  2:730007/140‑1 (MQ=255)                                                                                                                            aaCGCGCCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCAAGGTCAACTACAGCCAGATCGATCCGGCGTTATGTCGTGaa  >  1:665801/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                       TATTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGTGGGGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATTAAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAAAAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCAAGGTCAACTACAGCCAGATCGATCCGGCGTTATGTCGTGAA  >  NC_000913/1485039‑1485301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑