Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,618,444	T→C	V155V (GTA→GTG)	marC ←	inner membrane protein MarC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,618,444	0	T	C	100.0%	130.1 / NA	37	V155V (GTA→GTG)	marC	inner membrane protein MarC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/16);  total (21/16)

CAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAG  >  NC_000913/1618309‑1618578                                                                                                                                        |                                                                                                                                       cagGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCAccc                                                                                                                                    >  1:87885/1‑140 (MQ=255)  agGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCa                                                                                                                                   >  1:258982/1‑140 (MQ=255)  agGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCa                                                                                                                                   >  1:598449/1‑140 (MQ=255)        cccATCAGACGGGAGATCGCTTCAATTCCACCCTTTCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGc                                                                                                                             <  1:347788/140‑1 (MQ=255)        cccATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGTGGCGGCGCAACCATCAGCACCCAATCAGc                                                                                                                             >  1:133985/1‑140 (MQ=255)                   ggAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAAc                                                                                                                  >  2:429731/1‑140 (MQ=255)                          gCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACg                                                                                                           <  2:515690/140‑1 (MQ=255)                                 tGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCCTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACtgtg                                                                                                    >  2:397537/1‑140 (MQ=255)                                      cccTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGc                                                                                               >  1:76692/1‑140 (MQ=255)                                       ccTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGcc                                                                                              <  2:76692/140‑1 (MQ=255)                                             ccaccaGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCta                                                                                        >  1:404586/1‑140 (MQ=255)                                                 caGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCtaataa                                                                                    >  1:389711/1‑140 (MQ=255)                                                    ccGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAatca                                                                                 >  1:510796/1‑140 (MQ=255)                                                     cGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAatcat                                                                                <  2:404586/140‑1 (MQ=255)                                                     cGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAatcat                                                                                >  1:446291/1‑140 (MQ=255)                                                       cATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCg                                                                              <  2:667499/140‑1 (MQ=255)                                                               cgcCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGtt                                                                      >  1:697595/1‑140 (MQ=255)                                                               cgcCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGtt                                                                      <  1:429731/140‑1 (MQ=255)                                                                         gCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCgg                                                            <  2:272901/140‑1 (MQ=255)                                                                               gCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTAc                                                      >  2:68242/1‑140 (MQ=255)                                                                               gCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTAc                                                      >  1:212982/1‑140 (MQ=255)                                                                                    cccACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGc                                                 >  2:84438/1‑140 (MQ=255)                                                                                    cccACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGc                                                 >  1:365821/1‑140 (MQ=255)                                                                                         aaaaTAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGc                                            <  2:365821/140‑1 (MQ=255)                                                                                              aaCCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCg                                       >  1:750724/1‑140 (MQ=255)                                                                                                  gCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATCATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGcac                                   <  2:87885/140‑1 (MQ=255)                                                                                                     accaGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACaaa                                <  2:212982/140‑1 (MQ=255)                                                                                                     accaGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACaaa                                >  2:435699/1‑140 (MQ=255)                                                                                                        aGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGc                             <  2:750724/140‑1 (MQ=255)                                                                                                        aGGAAAAAGATCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGc                             <  1:418386/140‑1 (MQ=255)                                                                                                                  tCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCa                   <  1:68242/140‑1 (MQ=255)                                                                                                                  tCAGCGGCGGCGCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCa                   <  1:345647/140‑1 (MQ=255)                                                                                                                        gcggcgCAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGt             >  1:620043/1‑140 (MQ=255)                                                                                                                           gcgcAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCa          <  2:598449/140‑1 (MQ=255)                                                                                                                           gcgcAACCATCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACAGGGTTCa          >  1:819092/1‑140 (MQ=255)                                                                                                                                   aTCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAg  <  2:510796/140‑1 (MQ=255)                                                                                                                                   aTCAGCACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAg  >  1:804194/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       CAGGAATCCCATCAGACGGGAGATCGCTTCAATGCCACCCTTGCCCACCAGCCGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAG  >  NC_000913/1618309‑1618578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑