Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,720,255	T→C	V127A (GTA→GCA)	slyB →	outer membrane lipoprotein SlyB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,720,255	0	T	C	100.0%	101.8 / NA	29	V127A (GTA→GCA)	slyB	outer membrane lipoprotein SlyB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/17);  total (12/17)

ACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTT  >  NC_000913/1720119‑1720390                                                                                                                                         |                                                                                                                                        aCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGcaca                                                                                                                                      <  1:824151/140‑1 (MQ=255)          tctcTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAgg                                                                                                                             >  2:438360/1‑140 (MQ=255)          tctcTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAgg                                                                                                                             <  2:278912/140‑1 (MQ=255)                      gcagcaGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACaa                                                                                                                               >  2:421644/1‑126 (MQ=255)                      gcagcaGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACaa                                                                                                                               <  1:421644/126‑1 (MQ=255)                              cgcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCgg                                                                                                         >  1:78786/1‑140 (MQ=255)                               gcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCggg                                                                                                        <  1:438360/140‑1 (MQ=255)                               gcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCggg                                                                                                        >  2:44501/1‑140 (MQ=255)                                       aGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACgtg                                                                                                <  1:217632/140‑1 (MQ=255)                                        ggTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACgtgt                                                                                               >  1:718160/1‑140 (MQ=255)                                          tGGCGTAGCTGGTCAGGGTGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCg                                                                                             <  2:784393/140‑1 (MQ=255)                                            gCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTa                                                                                           <  2:449891/140‑1 (MQ=255)                                            gCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTa                                                                                           <  1:44501/140‑1 (MQ=255)                                            gCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTa                                                                                           <  2:78786/140‑1 (MQ=255)                                             cGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACgtg                                                                                                <  1:301453/134‑1 (MQ=255)                                             cGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACgtg                                                                                                >  2:301453/1‑134 (MQ=255)                                             cGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTAc                                                                                          >  1:539419/1‑140 (MQ=255)                                                    ggTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAg                                                                                   >  2:706517/1‑140 (MQ=255)                                                     gTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGc                                                                                  >  1:800287/1‑140 (MQ=255)                                                       cAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCaa                                                                                <  2:539419/140‑1 (MQ=255)                                                                                aaaCGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCcg                                                       <  2:800287/140‑1 (MQ=255)                                                                                 aaCGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCcgc                                                      <  1:706517/140‑1 (MQ=255)                                                                                  aCGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCcgcg                                                     <  2:628517/140‑1 (MQ=255)                                                                                             cGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGtt                                          >  2:540928/1‑140 (MQ=255)                                                                                                    gAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCgtgt                                   <  2:261027/140‑1 (MQ=255)                                                                                                        ttCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTc                               <  2:21034/140‑1 (MQ=255)                                                                                                                     tGGTAATACCCTCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCTCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACca                  <  2:718160/140‑1 (MQ=255)                                                                                                                         aaTACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACg              <  1:298929/140‑1 (MQ=255)                                                                                                                                 catGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTc      >  1:62838/1‑140 (MQ=255)                                                                                                                                  atGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGACAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCa     >  1:327014/1‑140 (MQ=255)                                                                                                                                     gtggcACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCAttt  <  1:297279/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        ACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTT  >  NC_000913/1720119‑1720390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑