Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,723,167	C→T	G349G (GGC→GGT)	ydhK →	putative transporter YdhK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,723,167	0	C	T	100.0%	142.6 / NA	41	G349G (GGC→GGT)	ydhK	putative transporter YdhK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/22);  total (19/22)

GTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTA  >  NC_000913/1723033‑1723303                                                                                                                                       |                                                                                                                                         gTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGc                                                                                                                                     <  2:98968/140‑1 (MQ=255)     ccGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGt                                                                                                                                 >  1:243169/1‑140 (MQ=255)       gACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCa                                                                                                                               >  1:682668/1‑140 (MQ=255)       gACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCa                                                                                                                               <  1:210076/140‑1 (MQ=255)                   gTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGc                                                                                                                   <  2:682668/140‑1 (MQ=255)                    tCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGcc                                                                                                                  >  1:182245/1‑140 (MQ=255)                          tCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCt                                                                                                            >  1:58034/1‑140 (MQ=255)                            tGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTAt                                                                                                          <  1:520495/140‑1 (MQ=255)                                    aCTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCg                                                                                                  <  2:736084/140‑1 (MQ=255)                                      tACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCggg                                                                                                <  2:112893/140‑1 (MQ=255)                                      tACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCggg                                                                                                <  1:648240/140‑1 (MQ=255)                                           tATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCg                                                                                           <  2:805072/140‑1 (MQ=255)                                           tATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCg                                                                                           <  2:332784/140‑1 (MQ=255)                                                  gccgccTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACAttt                                                                                    >  2:13667/1‑140 (MQ=255)                                                        tttATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACg                                                                              <  1:136828/140‑1 (MQ=255)                                                                cAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTtgatgat                                                                      >  1:485328/1‑140 (MQ=255)                                                                  aaGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTtgatgatga                                                                    <  2:58034/140‑1 (MQ=255)                                                                     tagtCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTg                                                                 >  1:34510/1‑140 (MQ=255)                                                                       gtCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGgc                                                               >  1:257087/1‑140 (MQ=255)                                                                       gtCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGgc                                                               <  2:243169/140‑1 (MQ=255)                                                                          aGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCa                                                            <  1:462054/140‑1 (MQ=255)                                                                          aGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCa                                                            >  2:577924/1‑140 (MQ=255)                                                                          aGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACCCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCa                                                            >  1:507341/1‑140 (MQ=255)                                                                            gAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATg                                                          <  1:577924/140‑1 (MQ=255)                                                                                       tCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCtt                                               >  1:78467/1‑140 (MQ=255)                                                                                                cGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATggg                                      <  2:78467/140‑1 (MQ=255)                                                                                                          gatCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGc                            <  1:13667/140‑1 (MQ=255)                                                                                                            tCATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCa                          <  2:182245/140‑1 (MQ=255)                                                                                                            tCATACCAGACCCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCa                          <  2:507341/140‑1 (MQ=255)                                                                                                             cATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCaa                         <  1:68852/140‑1 (MQ=255)                                                                                                             cATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCaa                         <  1:457/140‑1 (MQ=255)                                                                                                             cATACCAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCaa                         >  2:674105/1‑140 (MQ=255)                                                                                                                 ccAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCa                     >  2:313793/1‑140 (MQ=255)                                                                                                                 ccAGACTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCa                     >  1:538052/1‑140 (MQ=255)                                                                                                                     aCTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTaa                 >  1:504322/1‑140 (MQ=255)                                                                                                                     aCTCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTaa                 >  1:440093/1‑140 (MQ=255)                                                                                                                       tCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACg               >  2:310665/1‑140 (MQ=255)                                                                                                                       tCCCACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACg               <  1:674105/140‑1 (MQ=255)                                                                                                                          cACGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTg            >  2:342498/1‑140 (MQ=255)                                                                                                                            cGGACATCCGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGgc          >  1:608372/1‑140 (MQ=255)                                                                                                                                    cGGTCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTa  <  2:34510/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         GTCGCCGACTATCGGCACGTCGCCTTCTGGCAGCGACTACGTTATTTTTGCCGCCTTTATCTGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTATGTGGAGCGGGCTGCGTACATTTTGTACGTTGATGATGATTGGCGCATGGAGTATTGCTTCGCAATGGGATGCCGGTGCCAATGCATTAACGCTGGCAGCAATTA  >  NC_000913/1723033‑1723303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑