Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,780,993	T→C	V198A (GTT→GCT)	ydiR →	putative electron transfer flavoprotein subunit YdiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,780,993	0	T	C	100.0%	188.7 / NA	52	V198A (GTT→GCT)	ydiR	putative electron transfer flavoprotein subunit YdiR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/21);  total (31/21)

CCGTGCACCAGTGATA‑CCTCTCATCAGTGCCCGACAGAAACGGTACCTTATGTTGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGAGCGTGAACGTT  >  NC_000913/1780857‑1781124                                                                                                                                          |                                                                                                                                    ccGTGCACCAGTGATA‑CCTCTCATCAGTGCCCGACAGAAACGGTACCTTATGTTGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTgg                                                                                                                                  >  1:416812/1‑140 (MQ=255)     gCACCAGTGATACCCTCTCATCAGTGCCCGACAGAAACGGTACCTTATGTTGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCgt                                                                                                                               <  2:320476/140‑1 (MQ=255)      cACCAGTGATA‑CCTCTCATCAGTGCCCGACAGAAACGGTACCTTATGTTGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCgtcg                                                                                                                             >  2:636308/1‑140 (MQ=255)              aTA‑CCTCTCATCAGTGCCCGACAGAAACGGTACCTTATGTTGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGa                                                                                                                     >  2:276450/1‑140 (MQ=255)                   ctctCATCAGTGCCCGACAGAAACGGTACCTTATGTTGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTgg                                                                                                                 <  1:276450/140‑1 (MQ=255)                     ctcATCAGTGCCCGACAGAAACGGTACCTTATGTTGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTggcg                                                                                                               >  1:691877/1‑140 (MQ=255)                      tcatcaGTGCCCGACAGAAACGGTACCTTATGTTGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTggcgg                                                                                                              >  2:749469/1‑140 (MQ=255)                             tGCCCGACAGAAACGGTACCTTATGTTGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGa                                                                                                       >  1:736630/1‑140 (MQ=255)                             tGCCCGACAGAAACGGTACCTTATGTTGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGa                                                                                                       <  1:537211/140‑1 (MQ=255)                             tGCCCGACAGAAACGGTACCTTATGTTGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGa                                                                                                       >  2:367088/1‑140 (MQ=255)                               cccGACAGAAACGGTACCTTATGTTGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATg                                                                                                     >  2:299917/1‑140 (MQ=255)                                  gACAGAAACGGTACCTTATGTTGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGAcc                                                                                                  >  1:742828/1‑140 (MQ=255)                                               ccTTATGTTGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCa                                                                                     <  1:299917/140‑1 (MQ=255)                                                 ttATGTTGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATg                                                                                          <  2:187248/133‑1 (MQ=255)                                                 ttATGTTGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATg                                                                                          >  1:187248/1‑133 (MQ=255)                                                      ttGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTg                                                                              >  2:576499/1‑140 (MQ=255)                                                       tGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTgg                                                                             >  1:233375/1‑140 (MQ=255)                                                         cTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCa                                                                                     >  1:637288/1‑130 (MQ=255)                                                         cTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCa                                                                                     <  2:637288/130‑1 (MQ=255)                                                         cTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTggcg                                                                           >  1:155233/1‑140 (MQ=255)                                                         cTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTggcg                                                                           >  1:639826/1‑140 (MQ=255)                                                         cTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTgg                                                                             >  1:503515/1‑138 (MQ=255)                                                         cTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTgg                                                                             <  2:503515/138‑1 (MQ=255)                                                          tCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTggcgg                                                                          >  2:751600/1‑140 (MQ=255)                                                          tCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTggcgg                                                                          <  2:416812/140‑1 (MQ=255)                                                          tCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTggcgg                                                                          >  1:375412/1‑140 (MQ=255)                                                            cgcgTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGgcggcg                                                                        >  2:278125/1‑140 (MQ=255)                                                                   tGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGtgctga                                                                 <  1:367088/140‑1 (MQ=255)                                                                      aaTTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGtgctgaatg                                                              <  2:388809/140‑1 (MQ=255)                                                                                cgcgAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCgg                                                    <  2:75417/140‑1 (MQ=255)                                                                                cgcgAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCgg                                                    >  1:130957/1‑140 (MQ=255)                                                                                                 aGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGGCGGCTGTTCACGTCCAATTg                                   >  1:39967/1‑140 (MQ=255)                                                                                                      cAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGaa                              >  2:22163/1‑140 (MQ=255)                                                                                                         gcagcGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGc                           <  2:736630/140‑1 (MQ=255)                                                                                                           ggcGTGGACCTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCga                         <  1:678082/139‑1 (MQ=255)                                                                                                                    cTGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACtggat                <  2:691877/140‑1 (MQ=255)                                                                                                                     tGAGCAAAGCAAAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACtggatg               <  2:375412/140‑1 (MQ=255)                                                                                                                       agcaaagcaaAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACtggatgga             >  2:711294/1‑140 (MQ=255)                                                                                                                       agcaaagcaaAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACtggatgga             >  1:39061/1‑140 (MQ=255)                                                                                                                       agcaaagcaaAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGGGCTGAATGCTGGAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGGGAACtgggtggg             >  2:372040/1‑139 (MQ=255)                                                                                                                       agcaaagcaaAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGGGCTGAATGCTGAAGTCGGCTGTTCCCGTCCAATTGCCGAAGGCGGGAACtggatggg             >  2:644892/1‑139 (MQ=255)                                                                                                                       agcaaagcaaAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGGGCTGAATGCTGAAGTCGGCTGTTCACGTCCAAATGCCGAAGGCGGGAAatggatggg             >  2:794535/1‑139 (MQ=255)                                                                                                                       agcaaagcaaAACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGGGCTGAATGCTGAAATCGGCTGTTCACCTCCAATTGCCGAAGGCGGGAAatggatggg             >  2:678082/1‑139 (MQ=255)                                                                                                                            agcaaaACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGAGCGTg        <  1:749469/140‑1 (MQ=255)                                                                                                                            agcaaaACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGAGCGTg        <  1:21201/140‑1 (MQ=255)                                                                                                                            agcaaaACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCCGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGAGCGTg        <  1:278125/140‑1 (MQ=255)                                                                                                                            agcaaaACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGGGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGAGCGTg        >  2:21201/1‑140 (MQ=255)                                                                                                                            agcaaaACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACCGGCGGCGGGGCTGAAAGCTGAAGGCGGCTGTTCACGTCCAAATGCCGAAAGCGAGAACTGGGTGGGGCggg        >  2:816397/1‑138 (MQ=255)                                                                                                                              caaaACGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGAGCGTGaa      <  1:22163/140‑1 (MQ=255)                                                                                                                                 aaCGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGAGCGTGAACg    >  2:228430/1‑139 (MQ=255)                                                                                                                                 aaCGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGAGCGTGAACg    <  1:228430/139‑1 (MQ=255)                                                                                                                                  aCGTGTGGCTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGAGCGTGAACGtt  <  1:636308/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    CCGTGCACCAGTGATA‑CCTCTCATCAGTGCCCGACAGAAACGGTACCTTATGTTGCTCCGCGTCATGAAATTCTCTGTCGCGAACGCCGTGCGAAAGCCGCAAGCAGCGTGGACCTGAGCAAAGCAAAACGTGTGGTTGGCGTCGGTCGTGGACTGGCGGCGCAGGATGACCTAAAAATGGTCCACGAACTGGCGGCGGTGCTGAATGCTGAAGTCGGCTGTTCACGTCCAATTGCCGAAGGCGAGAACTGGATGGAGCGTGAACGTT  >  NC_000913/1780857‑1781124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑