Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,846,333	C→T	D210N (GAT→AAT)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,846,333	0	C	T	100.0%	160.8 / NA	45	D210N (GAT→AAT)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/24);  total (21/24)

GCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCT  >  NC_000913/1846196‑1846466                                                                                                                                          |                                                                                                                                      gcgcCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATtgc                                                                                                                                     >  2:719616/1‑140 (MQ=255)   gcCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTgcgg                                                                                                                                   >  2:624707/1‑140 (MQ=255)   gcCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTgcgg                                                                                                                                   >  2:450910/1‑140 (MQ=255)    cccTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTgcggc                                                                                                                                  >  1:431840/1‑140 (MQ=255)      cttcttCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGgcgc                                                                                                                                >  1:671176/1‑140 (MQ=255)           tcATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGcaccac                                                                                                                           <  2:562365/140‑1 (MQ=255)           tcATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGcaccac                                                                                                                           <  2:155798/140‑1 (MQ=255)           tcATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGcaccac                                                                                                                           <  1:750337/140‑1 (MQ=255)           tcATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGcaccac                                                                                                                           <  1:624707/140‑1 (MQ=255)                cTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGcc                                                                                                                      <  2:829510/140‑1 (MQ=255)                   gTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCaa                                                                                                                   >  2:640041/1‑140 (MQ=255)                    tACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAg                                                                                                                  >  2:488060/1‑140 (MQ=255)                         ttCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACgg                                                                                                             >  2:559422/1‑140 (MQ=255)                         ttCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACgg                                                                                                             >  1:552620/1‑140 (MQ=255)                         ttCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACgg                                                                                                             >  2:677271/1‑140 (MQ=255)                         ttCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACgg                                                                                                             >  1:133980/1‑140 (MQ=255)                              acGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTc                                                                                                        >  1:324285/1‑140 (MQ=255)                                 cTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGc                                                                                                     >  2:784298/1‑140 (MQ=255)                                   tCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCAc                                                                                                   >  1:12684/1‑140 (MQ=255)                                        cGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTc                                                                                              <  2:12684/140‑1 (MQ=255)                                         ggTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTcc                                                                                             <  1:70406/140‑1 (MQ=255)                                            tGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCAc                                                                                          >  1:430463/1‑140 (MQ=255)                                             gCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACg                                                                                         <  1:577174/140‑1 (MQ=255)                                              ccAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACgg                                                                                        <  1:719616/140‑1 (MQ=255)                                                      cGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACg                                                                                <  1:394424/140‑1 (MQ=255)                                                      cGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACg                                                                                <  1:281830/140‑1 (MQ=255)                                                      cGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACg                                                                                <  1:230030/140‑1 (MQ=255)                                                             ccGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCCCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGAt                                                                         <  2:186676/140‑1 (MQ=255)                                                                           gtgtCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGa                                                           <  2:543565/140‑1 (MQ=255)                                                                            tgtCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGAc                                                          <  1:450910/140‑1 (MQ=255)                                                                              tCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCg                                                        >  1:439605/1‑140 (MQ=255)                                                                                 cGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCgaga                                                     <  1:559422/140‑1 (MQ=255)                                                                                  gATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGaa                                                    <  2:302932/140‑1 (MQ=255)                                                                                  gATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGaa                                                    <  1:488060/140‑1 (MQ=255)                                                                                   atatGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAAt                                                   <  2:431840/140‑1 (MQ=255)                                                                                   atatGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAAt                                                   <  2:133980/140‑1 (MQ=255)                                                                                      tgtgCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGaa                                                    <  1:19554/136‑1 (MQ=255)                                                                                      tgtgCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGaa                                                    >  2:19554/1‑136 (MQ=255)                                                                                      tgtgCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGt                                                >  1:678067/1‑140 (MQ=255)                                                                                      tgtgCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGt                                                <  1:640041/140‑1 (MQ=255)                                                                                                 ttCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGtat                                              >  2:474971/1‑131 (MQ=255)                                                                                                 ttCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGtat                                              <  1:474971/131‑1 (MQ=255)                                                                                                  tCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGt                                    <  1:677271/140‑1 (MQ=255)                                                                                                   cAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTc                                   >  1:564259/1‑140 (MQ=255)                                                                                                             tttCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGc                         <  2:439605/140‑1 (MQ=255)                                                                                                             tttCGCCACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGc                         >  2:307363/1‑140 (MQ=255)                                                                                                                  ccACGAAGTTTTCAATCTCTTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTAc                    >  2:302540/1‑140 (MQ=255)                                                                                                                                    ctTCATTGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCt  >  2:380525/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      GCGCCCTTCTTCATCCTGGTACGGTTCACACGCTTCGCTCGGTTGCCAGATAGCGGTAAACCGCTCATCGGCAGGTGTCACGATATGTGCTTTGACTTCAAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAGCCAGTCGGCACGCGCT  >  NC_000913/1846196‑1846466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑