Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,901,792 G→A intergenic (‑10/‑256) yoaL ← / → manX protein YoaL/mannose‑specific PTS enzyme IIAB component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,901,7920GA100.0% 165.5 / NA 48intergenic (‑10/‑256)yoaL/manXprotein YoaL/mannose‑specific PTS enzyme IIAB component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/20);  total (28/20)

GCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCT  >  NC_000913/1901666‑1901922
                                                                                                                              |                                                                                                                                  
gCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGc                                                                                                                       <  1:809114/140‑1 (MQ=255)
gCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGc                                                                                                                       <  2:512237/140‑1 (MQ=255)
    agagTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAg                                                                                                                   <  1:725660/140‑1 (MQ=255)
                                   tGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACaa                                                                                    <  2:548190/140‑1 (MQ=255)
                                   tGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACaa                                                                                    <  2:45181/140‑1 (MQ=255)
                                    gATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAg                                                                                   <  1:546551/140‑1 (MQ=255)
                                                aCGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCt                                                                       >  1:305390/1‑140 (MQ=255)
                                                aCGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCt                                                                       >  2:264247/1‑140 (MQ=255)
                                                aCGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCATCCTTTGCAAACGAATGTGACAAGGATATTTTACCt                                                                       >  2:394557/1‑140 (MQ=255)
                                                  gTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCttt                                                                     >  1:264543/1‑140 (MQ=255)
                                                   tCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTc                                                                    >  2:640190/1‑140 (MQ=255)
                                                                      cGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGaa                                                 >  2:677080/1‑140 (MQ=255)
                                                                        ggCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAg                                               >  2:710326/1‑140 (MQ=255)
                                                                                cccTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTa                                       >  2:402948/1‑140 (MQ=255)
                                                                                cccTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTa                                       <  1:640190/140‑1 (MQ=255)
                                                                                cccTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTa                                       >  2:715309/1‑140 (MQ=255)
                                                                                     taCTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACg                                     <  1:325288/137‑1 (MQ=255)
                                                                                     taCTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACg                                     >  2:325288/1‑137 (MQ=255)
                                                                                     aaCTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTCCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGAt                                  <  2:390682/139‑1 (MQ=255)
                                                                                          cATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTGCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGAt                                  <  2:576048/135‑1 (MQ=255)
                                                                                          cATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAAt                                         >  1:81762/1‑128 (MQ=255)
                                                                                          cATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAAt                                         <  2:81762/128‑1 (MQ=255)
                                                                                          cATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGAt                                  >  1:576048/1‑135 (MQ=255)
                                                                                          cATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTtca                             >  2:527713/1‑140 (MQ=255)
                                                                                          cATGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTtca                             >  2:243647/1‑140 (MQ=255)
                                                                                          cATGGTTGTTAAACGGGAGTTAAACA‑ATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACgg                                    >  2:311694/1‑132 (MQ=255)
                                                                                          cATGGTTGTTAAACGGGAGTTAAACA‑ATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACgg                                    <  1:311694/132‑1 (MQ=255)
                                                                                            tGGTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTtcatc                           >  1:408643/1‑140 (MQ=255)
                                                                                             ggTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTAc                                      >  1:35534/1‑128 (MQ=255)
                                                                                             ggTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTAc                                      <  2:35534/128‑1 (MQ=255)
                                                                                             ggTTGTTAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTtcatca                          >  1:184030/1‑140 (MQ=255)
                                                                                               ttgttAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAGTGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATcaca                        >  2:577007/1‑140 (MQ=255)
                                                                                               ttgttAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGAt                                  <  2:324843/130‑1 (MQ=255)
                                                                                               ttgttAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGAt                                  >  1:324843/1‑130 (MQ=255)
                                                                                               ttgttAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATcaca                        >  1:224578/1‑140 (MQ=255)
                                                                                               ttgttAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATcaca                        >  1:306356/1‑140 (MQ=255)
                                                                                               ttgttAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATcaca                        >  1:662698/1‑140 (MQ=255)
                                                                                                 gttAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTtcat                            <  1:511119/134‑1 (MQ=255)
                                                                                                 gttAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTtcat                            >  2:511119/1‑134 (MQ=255)
                                                                                                 gttAAACGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATa                      >  2:325828/1‑140 (MQ=255)
                                                                                                       cGGGAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAaataa                >  2:72346/1‑140 (MQ=255)
                                                                                                        gggAGTTAAACATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAaataat               >  2:814632/1‑140 (MQ=255)
                                                                                                               aaaCATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCg        <  2:562010/140‑1 (MQ=255)
                                                                                                               aaaCATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCg        <  1:715309/140‑1 (MQ=255)
                                                                                                               aaaCATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCg        <  1:325828/140‑1 (MQ=255)
                                                                                                               aaaCATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCg        <  2:305390/140‑1 (MQ=255)
                                                                                                               aaaCATATCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCg        <  2:306356/140‑1 (MQ=255)
                                                                                                                     atCAGAGACACCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCt  >  1:158492/1‑140 (MQ=255)
                                                                                                                              |                                                                                                                                  
GCATAGAGTGCCAGAAAGGCAGGCGTTAAAAGGCCTGATGCTGAAATGACGTCGGTGACGATCCATACTGCGGGCTACTGCCCTATACTCCATGGTTGTTAAACGGGAGTTAAACATATCAGAGACGCCTCTGATTTGGCAAAGATTTACCTTCCTTTGCAAACGAATGTGACAAGGATATTTTACCTTTCGAAATTTCTGCTAATCGAAAGTTAAATTACGGATCTTCATCACATAAAATAATTTTTTCGATATCT  >  NC_000913/1901666‑1901922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑