Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,924,381	T→C	T71A (ACG→GCG)	yebZ ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,924,381	0	T	C	100.0%	69.8 / NA	20	T71A (ACG→GCG)	yebZ	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/10);  total (10/10)

CATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGTTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGCAGAAAACGCTTCGCCAGCAAGCGACGAATCGTCAAGGGGGCCAGCCAGGCACCGTA  >  NC_000913/1924247‑1924516                                                                                                                                       |                                                                                                                                        cATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCa                                                                                                                                    <  2:205446/140‑1 (MQ=255)      tCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAAc                                                                                                                               <  2:392385/140‑1 (MQ=255)           ggTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGcc                                                                                                                          <  1:292055/140‑1 (MQ=255)                    aGGGCGACGATCAACGTTACAAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGc                                                                                                                 <  1:167085/140‑1 (MQ=255)                                         aGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGAt                                                                                            <  1:181710/140‑1 (MQ=255)                                            gCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCaa                                                                                         >  2:811044/1‑140 (MQ=255)                                                            aTAACCAGATGCCACCAAACTGCGTTTGC‑‑AACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTg                                                                       <  2:800541/140‑1 (MQ=255)                                                                    aTGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCtgttg                                                                 >  1:234701/1‑140 (MQ=255)                                                                        caccaAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTaaa                                                             >  1:279802/1‑140 (MQ=255)                                                                         accaAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAAc                                                            >  2:416965/1‑140 (MQ=255)                                                                          ccaAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACg                                                           >  2:293676/1‑140 (MQ=255)                                                                           caAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGc                                                          >  1:400927/1‑140 (MQ=255)                                                                             aaCTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGCAg                                                        >  1:458168/1‑140 (MQ=255)                                                                                                ccTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGCAGAAAACGCTTCGCCAGCAAg                                     >  1:481629/1‑140 (MQ=255)                                                                                                        aTGTTTGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGCAGAAAACGCTTCGCCAGCAAGCGACGAAt                             >  2:124616/1‑140 (MQ=255)                                                                                                             tGGCGAAAATACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGCAGAAAACGCTTCGCCAGCAAGCGACGAATCGTCa                        <  2:458168/140‑1 (MQ=255)                                                                                                                  aaaaTACATCTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGCAGAAAACGCTTCGCCAGCAAGCGACGAATCGTCAAgggg                   <  1:293676/140‑1 (MQ=255)                                                                                                                           cTGTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCATCGTGCTGTTGTAAACGCAGAAAACGCTTCGCCAGCAAGCGACGAATCGTCAAGGGGGccagccag          <  2:710250/140‑1 (MQ=255)                                                                                                                             gTCCATCCCGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGCAGAAAACGCTTCGCCAGCAAGCGACGAATCGTCAAGGGGGCCAGCCAGGc        <  1:274692/140‑1 (MQ=255)                                                                                                                                   cccGCTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGCAGAAAACGCTTCGCCAGCAAGCGACGAATCGTCAAGGGGGCCAGCCAGGCACCGTa  >  1:794302/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        CATATTCCGCGGTTGCATAAGGGCGACGATCAACGTTACGAGGGCGAGAACAATTTGCCATAACCAGATGCCACCAAACTGCGTTTGCAAAACAGCCTGCCAAATGTTTGGCGAAAATACATCTGTCCATCCCGTTCCCATCAACCCGCCCTGAACGGCGAGCATTGCAGTGGCACTGATCAAACTCCAGACGGCAGCGTGCTGTTGTAAACGCAGAAAACGCTTCGCCAGCAAGCGACGAATCGTCAAGGGGGCCAGCCAGGCACCGTA  >  NC_000913/1924247‑1924516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑