Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,015,676	C→T	A266V (GCC→GTC)	fliG →	flagellar motor switch protein FliG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,015,676	0	C	T	100.0%	96.5 / NA	30	A266V (GCC→GTC)	fliG	flagellar motor switch protein FliG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/15);  total (15/15)

ACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGC  >  NC_000913/2015544‑2015807                                                                                                                                     |                                                                                                                                    aCGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGagcag                                                                                                                              >  1:343360/1‑140 (MQ=255)  cGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGagcagc                                                                                                                             <  1:787011/140‑1 (MQ=255)          tGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTgcgc                                                                                                                     >  1:270708/1‑140 (MQ=255)            gcgcAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCga                                                                                                                   >  1:248138/1‑140 (MQ=255)                            acgaGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAat                                                                                                   >  1:462457/1‑140 (MQ=255)                                       cTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCg                                                                                        >  1:660470/1‑140 (MQ=255)                                         gTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTg                                                                                      >  2:683887/1‑140 (MQ=255)                                            cGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTgccg                                                                                   <  2:5004/140‑1 (MQ=255)                                              agaATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTgccgcc                                                                                 >  2:270220/1‑140 (MQ=255)                                                    tggtggATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATAtt                                                                           >  1:234431/1‑140 (MQ=255)                                                     ggtggATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTc                                                                          >  2:657281/1‑140 (MQ=255)                                                              cgacgaTCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGcgacg                                                                 <  2:155226/140‑1 (MQ=255)                                                                     cGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGc                                                          <  1:174642/140‑1 (MQ=255)                                                                      gcagcaTTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGcc                                                         <  2:298076/140‑1 (MQ=255)                                                                       cagcaTTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCa                                                        >  2:133648/1‑140 (MQ=255)                                                                            ttCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGt                                                   <  1:334799/140‑1 (MQ=255)                                                                            ttCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGt                                                   >  2:819059/1‑140 (MQ=255)                                                                              cAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTgg                                                 <  2:248138/140‑1 (MQ=255)                                                                                    cTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGAtct                                                             <  1:611405/122‑1 (MQ=255)                                                                                    cTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGAtct                                                             >  2:611405/1‑122 (MQ=255)                                                                                    cTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGt                                           <  2:480814/140‑1 (MQ=255)                                                                                       ttGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCg                                        >  1:354987/1‑140 (MQ=255)                                                                                              aaGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCg                                 <  1:133648/140‑1 (MQ=255)                                                                                              aaGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCg                                 <  2:660470/140‑1 (MQ=255)                                                                                               aGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGc                                <  1:270220/140‑1 (MQ=255)                                                                                                     ttCCGAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTgg                          <  1:750584/140‑1 (MQ=255)                                                                                                         gAATCGCTGTTGATCGCGCTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGaaaa                      <  2:234431/140‑1 (MQ=255)                                                                                                                          gcTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTc     >  1:605400/1‑140 (MQ=255)                                                                                                                          gcTGAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTc     >  2:401390/1‑140 (MQ=255)                                                                                                                             gAAAGGAGTCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTctgc  <  2:605400/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    ACGGCGAGCTGGCGCAGAAAATCATCGACGAGATGTTCCTGTTCGAGAATCTGGTGGATGTCGACGATCGCAGCATTCAGCGTCTGTTGCAGGAAGTGGATTCCGAATCGCTGTTGATCGCGCTGAAAGGAGCCGAGCAGCCACTGCGCGAGAAATTCTTGCGCAATATGTCGCAGCGTGCCGCCGATATTCTGCGCGACGATCTCGCCAACCGTGGTCCGGTGCGTCTGTCGCAGGTGGAAAACGAACAGAAAGCGATTCTGC  >  NC_000913/2015544‑2015807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑