Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,086,480	A→G	S195P (TCT→CCT)	plaP ←	putrescine:H(+) symporter PlaP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,086,480	0	A	G	100.0%	135.5 / NA	37	S195P (TCT→CCT)	plaP	putrescine:H(+) symporter PlaP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/20);  total (17/20)

AGGAAAATCGCACGCGGGATCACGCGCTCTGCATCTTTGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATT  >  NC_000913/2086344‑2086614                                                                                                                                         |                                                                                                                                       aGGAAAATCGCACGCGGGATCACGCGCTCTGCATCTTTGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGccaggcca                                                                                                                                     <  1:327/140‑1 (MQ=255) aGGAAAATCGCACGCGGGATCACGCGCTCTGCATCTTTGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGccaggcca                                                                                                                                     <  1:798433/140‑1 (MQ=255)         cGCACGCGGGATCACGCGCTCTGCATCTTTGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGAc                                                                                                                             <  2:609490/140‑1 (MQ=255)           cACGCGGGATCACGCGCTCTGCATCTTTGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGa                                                                                                                           >  1:330402/1‑140 (MQ=255)                 ggATCACGCGCTCTGCATCTTTGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTc                                                                                                                     <  2:726748/140‑1 (MQ=255)                 ggATCACGCGCTCTGCATCTTTGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTc                                                                                                                     <  1:419318/140‑1 (MQ=255)                        gcgcTCTTCATCTTTGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCg                                                                                                              >  1:1654/1‑140 (MQ=255)                                    tttGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGcctt                                                                                                  <  1:457269/140‑1 (MQ=255)                                      tGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGGGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGccttca                                                                                                >  1:445538/1‑140 (MQ=255)                                          ttcttcCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGccttcacctt                                                                                            >  2:727568/1‑140 (MQ=255)                                             ttcCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCaa                                                                                         >  1:181780/1‑140 (MQ=255)                                              tcCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCaaa                                                                                        <  1:185888/140‑1 (MQ=255)                                                  aCAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACg                                                                                    <  1:705421/140‑1 (MQ=255)                                                  aCAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACg                                                                                    >  2:433741/1‑140 (MQ=255)                                                  aCAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACg                                                                                    <  1:642474/140‑1 (MQ=255)                                                  aCAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACg                                                                                    <  1:309656/140‑1 (MQ=255)                                                      gTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCAt                                                                                >  1:628344/1‑140 (MQ=255)                                                         gCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATaaa                                                                             <  2:1654/140‑1 (MQ=255)                                                               gCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACg                                                                                    <  1:143585/127‑1 (MQ=255)                                                               gCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACg                                                                                    >  2:143585/1‑127 (MQ=255)                                                                 cGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATg                                                                     >  1:447324/1‑140 (MQ=255)                                                                   tCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGcc                                                                   <  1:433741/140‑1 (MQ=255)                                                                             tAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCAcc                                                         <  1:543881/140‑1 (MQ=255)                                                                                aGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACcgcg                                                      <  1:607063/140‑1 (MQ=255)                                                                                 ggAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGa                                                     <  1:25948/140‑1 (MQ=255)                                                                                 ggAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGa                                                     <  1:727568/140‑1 (MQ=255)                                                                                  gagaAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGAt                                                    <  2:330402/140‑1 (MQ=255)                                                                                   agaAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGACTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATc                                                   >  1:659066/1‑140 (MQ=255)                                                                                     aaGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAg                                                 <  1:416121/140‑1 (MQ=255)                                                                                             gATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGACTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGcggtgatt                                         <  2:659066/140‑8 (MQ=255)                                                                                               ttGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGAAGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGc                                       <  2:234873/140‑1 (MQ=255)                                                                                                  tCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAac                                    >  2:390316/1‑140 (MQ=255)                                                                                                  tCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAac                                    >  2:705197/1‑140 (MQ=255)                                                                                                    gCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAacac                                  <  1:25491/140‑1 (MQ=255)                                                                                                              tcatcGGGATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACa                        <  2:445538/140‑1 (MQ=255)                                                                                                                    ggATAACATGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTg                  >  2:653703/1‑140 (MQ=255)                                                                                                                            tGCGCATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGc          >  2:373391/1‑140 (MQ=255)                                                                                                                               gcATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTAc       >  2:694387/1‑140 (MQ=255)                                                                                                                                cATCGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACg      >  1:21833/1‑140 (MQ=255)                                                                                                                                   cGCCAGGCCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGAt   >  2:415804/1‑140 (MQ=255)                                                                                                                                    gccaggccagAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGAtt  <  2:181780/140‑1 (MQ=255)                                                                                                                                    gccaggccagAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGAtt  >  2:76415/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       AGGAAAATCGCACGCGGGATCACGCGCTCTGCATCTTTGGTTTCTTCCGACAGGTTGCTGATGCCGTCAAAGCCGGTAAAGGAGAAGCACAGGATTGTCGCCCCGGTAATCATCGGGATAACATGCGCATCGCCAGACCAGAATGGACGAGTGCTCGCCAGCGTACCAGCGCCTTCACCTTCAAATACGCCATAAACAACCATGCCCAGAATCACCGCGATCAGCACTACCTGCAACACGACGATTACAGTGTTGAAGTTCGCTACGGATT  >  NC_000913/2086344‑2086614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑