Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,242,011 A→G C319C (TGT→TGC)  yeiB ← DUF418 domain‑containing protein YeiB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,242,0110AG97.1% 116.6 / NA 34C319C (TGT→TGC) yeiBDUF418 domain‑containing protein YeiB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (15/18);  minor base T (1/0);  total (16/18)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGC  >  NC_000913/2241877‑2242146
                                                                                                                                      |                                                                                                                                       
aCCGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAAt                                                                                                                                    <  2:344670/140‑1 (MQ=255)
  cGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCa                                                                                                                                  <  2:672607/140‑1 (MQ=255)
      cccTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTGCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGt                                                                                                                              <  1:577481/140‑1 (MQ=255)
      cccTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGt                                                                                                                              <  1:42680/140‑1 (MQ=255)
         tGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTg                                                                                                                           <  2:69960/140‑1 (MQ=255)
                aGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAataga                                                                                                                    <  1:591855/140‑1 (MQ=255)
                           cAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAg                                                                                                         <  1:207599/140‑1 (MQ=255)
                           cAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACACCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAg                                                                                                         >  2:627867/1‑140 (MQ=255)
                            aGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAgc                                                                                                        >  2:418537/1‑140 (MQ=255)
                                aaCAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCa                                                                                                    <  1:627867/140‑1 (MQ=255)
                                    gagaAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCAtccg                                                                                                >  2:33434/1‑140 (MQ=255)
                                     agaagaGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTTCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCAtccgt                                                                                               >  2:763493/1‑140 (MQ=255)
                                          agGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGAc                                                                                          >  2:660930/1‑140 (MQ=255)
                                                   gccagccaTACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGAt                                                                                 >  2:246047/1‑140 (MQ=255)
                                                    ccagccaTACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATc                                                                                >  2:161057/1‑140 (MQ=255)
                                                       gccaTACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCa                                                                             >  2:440916/1‑140 (MQ=255)
                                                                     aCAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTaaa                                                               >  2:265216/1‑140 (MQ=255)
                                                                      cAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAg                                                              >  2:221382/1‑140 (MQ=255)
                                                                      cAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAg                                                              <  1:33434/140‑1 (MQ=255)
                                                                           gCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCt                                                         <  1:246047/140‑1 (MQ=255)
                                                                                       aGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCgg                                                <  2:697540/137‑1 (MQ=255)
                                                                                       aGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCgg                                                >  1:697540/1‑137 (MQ=255)
                                                                                       aGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCa                                             <  1:763493/140‑1 (MQ=255)
                                                                                       aGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCa                                             <  1:418537/140‑1 (MQ=255)
                                                                                         gCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGa                                           <  1:440916/140‑1 (MQ=255)
                                                                                         gCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGa                                           <  2:687135/140‑1 (MQ=255)
                                                                                                       tAAACAAACCGAGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGc                             <  1:26990/140‑1 (MQ=255)
                                                                                                                  aGGTGGTAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGcc                  >  2:519112/1‑140 (MQ=255)
                                                                                                                    gtggtAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCaa                <  1:161057/140‑1 (MQ=255)
                                                                                                                      ggtAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATc              >  2:604714/1‑140 (MQ=255)
                                                                                                                      ggtAAAAAAGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATc              >  1:25244/1‑140 (MQ=255)
                                                                                                                             aaGCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGaa       <  1:660930/140‑1 (MQ=255)
                                                                                                                               gCGTGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAAc     >  2:232414/1‑140 (MQ=255)
                                                                                                                                 gtggtgCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACgg   <  1:265216/140‑1 (MQ=255)
                                                                                                                                  tGGTGCAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGgc  >  1:542863/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                       
ACCGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGC  >  NC_000913/2241877‑2242146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑