Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,303,802	T→C	intergenic (+32/‑103)	yojO → / → eco	uncharacterized protein YojO/serine protease inhibitor ecotin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,303,802	0	T	C	100.0%	121.7 / NA	34	intergenic (+32/‑103)	yojO/eco	uncharacterized protein YojO/serine protease inhibitor ecotin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/16);  total (18/16)

CAAAAACGGGCTTTTGCTCACATTTCAAATGGTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGTTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGACATCATCGGTGAAAACCTATAAATGAAGAAGGAAAGCAAAAAAATGAAGACCATTCTACCTGCAGTATTGTTTG  >  NC_000913/2303666‑2303935                                                                                                                                         |                                                                                                                                      cAAAAACGGGCTTTTGCTCACATTTCAAATGGTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGctct                                                                                                                                    <  2:563268/140‑1 (MQ=255)   aaaaCGGGCTTTTGCTCACATTTCAAATGGTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCttt                                                                                                                                  <  1:405287/140‑1 (MQ=255)        gtgCTTTTGCTCACATTTCAAATGGTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGtt                                                                                                                             <  1:393204/138‑1 (MQ=255)                     caTTTCAAATGGTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCaa                                                                                                                <  1:692313/140‑1 (MQ=255)                      aTTTCAAATGGTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCaaa                                                                                                               >  1:231420/1‑140 (MQ=255)                        ttCAAATGGTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAAtt                                                                                                             >  1:225553/1‑140 (MQ=255)                                gTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCaa                                                                                                                >  1:191778/1‑129 (MQ=255)                                gTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCaa                                                                                                                <  2:191778/129‑1 (MQ=255)                                 ttATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCt                                                                                                    <  1:65538/140‑1 (MQ=255)                                  tataAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCtg                                                                                                   <  1:419768/140‑1 (MQ=255)                                  tataAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCtg                                                                                                   >  1:634274/1‑140 (MQ=255)                                               atataGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTaa                                                                                      <  2:231420/140‑1 (MQ=255)                                                     cGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCa                                                                                >  2:491056/1‑140 (MQ=255)                                                       attgattCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGc                                                                              >  2:706263/1‑140 (MQ=255)                                                       attgattCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGc                                                                              >  1:141134/1‑140 (MQ=255)                                                       attgattCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGc                                                                              >  2:454017/1‑140 (MQ=255)                                                              cACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGAcatca                                                                       <  1:454017/140‑1 (MQ=255)                                                               aCCAGAGATATTTCTCCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGAcatcat                                                                      >  2:63125/1‑140 (MQ=255)                                                                     gaTATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGACATCATCGGTGa                                                                >  1:20037/1‑140 (MQ=255)                                                                      atatTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGAcatcat                                                                      >  1:656165/1‑133 (MQ=255)                                                                      atatTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGAcatcat                                                                      <  2:656165/133‑1 (MQ=255)                                                                      atatTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGACATCATCGGTGaa                                                               <  2:634274/140‑1 (MQ=255)                                                                          ttCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGACATCATCGGTGAAAAcc                                                           >  1:176139/1‑140 (MQ=255)                                                                                     tGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGACATCATCGGTGAAAACCTATAAATgaag                                                >  2:757863/1‑140 (MQ=255)                                                                                            tcATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGACATCATCGGTGAAAACCTATAAATGAAGAAGGaaa                                         >  1:195952/1‑140 (MQ=255)                                                                                            tcATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGACATCATCGGTGAAAACCTATAAATGAAGAAGGaaa                                         >  1:813400/1‑140 (MQ=255)                                                                                                 aGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGACATCATCGGTGAAAACCTATAAATGAAGAAGGAAAGCaaa                                    >  2:475407/1‑140 (MQ=255)                                                                                                          cacTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGACATCATCGGTGAAAACCTATAAATGAAGAAGGAAAGCAAAAAAATGAAg                           <  1:757863/140‑1 (MQ=255)                                                                                                          cacTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGACATCATCGGTGAAAACCTATAAATGAAGAAGGAAAGCAAAAAAATGAAg                           <  2:176139/140‑1 (MQ=255)                                                                                                          cacTAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGACATCATCGGTGAAAACCTATAAATGAAGAAGGAAAGCAAAAAAATGAAg                           <  1:63125/140‑1 (MQ=255)                                                                                                             tAAAAGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGACATCATCGGTGAAAACCTATAAATGAAGAAGGAAAGCAAAAAAATGAAGAcc                        >  2:818322/1‑140 (MQ=255)                                                                                                                aaGAGCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGACATCATCGGTGAAAACCTATAAATGAAGAAGGAAAGCAAAAAAATGAAGACCAtt                     <  2:225553/140‑1 (MQ=255)                                                                                                                    gCAGGTAAAATTGTCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGACATCATCGGTGAAAACCTATAAATGAAGAAGGAAAGCAAAAAAATGAAGACCATTCTAc                 <  2:813400/140‑1 (MQ=255)                                                                                                                                 tCTGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGACATCATCGGTGAAAACCTATAAATGAAGAAGGAAAGCAAAAAAATGAAGACCATTCTACCTGCAGTATTGtt    <  2:195952/140‑1 (MQ=255)                                                                                                                                   tGAATGCTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGACATCATCGGTGAAAACCTATAAATGAAGAAGGAAAGCAAAAAAATGAAGACCATTCTACCTGCAGTATTGTTTg  >  1:196399/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      CAAAAACGGGCTTTTGCTCACATTTCAAATGGTTATAAATATATTTATATAGCGATTGATTCACCAGAGATATTTCTGCTGGTTTGCTCTCTCATTAGAATTTAACACTAAAAGAGCAGGTAAAATTGTCTGAATGTTCTTTAAGTTATTCATAAAGCAAATTAATAAATCTGATGAATATGTTAACCTTCAGCGACATCATCGGTGAAAACCTATAAATGAAGAAGGAAAGCAAAAAAATGAAGACCATTCTACCTGCAGTATTGTTTG  >  NC_000913/2303666‑2303935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑