Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,307,508	T→C	T370A (ACG→GCG)	yojI ←	ABC transporter family protein/microcin J25 efflux protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,307,508	0	T	C	100.0%	135.2 / NA	37	T370A (ACG→GCG)	yojI	ABC transporter family protein/microcin J25 efflux protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/20);  total (17/20)

CGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAG  >  NC_000913/2307371‑2307643                                                                                                                                          |                                                                                                                                        cGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCca                                                                                                                                       >  1:729063/1‑140 (MQ=255)  ggCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCcaa                                                                                                                                      >  2:184399/1‑140 (MQ=255)         gcagTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATc                                                                                                                               >  2:780665/1‑140 (MQ=255)             ttGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCa                                                                                                                           >  2:95252/1‑140 (MQ=255)                      cAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGAtt                                                                                                                  <  2:584528/140‑1 (MQ=255)                       aGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGAttt                                                                                                                 >  2:73397/1‑140 (MQ=255)                                           tGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCaa                                                                                             <  1:184399/140‑1 (MQ=255)                                           tGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCaa                                                                                             >  2:812203/1‑140 (MQ=255)                                           tGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCaa                                                                                             >  2:347212/1‑140 (MQ=255)                                            gCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTgccgc                                                                                                >  2:413476/1‑136 (MQ=255)                                            gCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTgccgc                                                                                                <  1:413476/136‑1 (MQ=255)                                                  aaCAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGaaa                                                                                      >  2:560861/1‑140 (MQ=255)                                                        ttGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAAcagcag                                                                                <  1:780665/140‑1 (MQ=255)                                                              tAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGcc                                                                          <  2:162904/140‑1 (MQ=255)                                                              tAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGcc                                                                          <  1:4211/140‑1 (MQ=255)                                                                 tCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACg                                                                       >  2:169798/1‑140 (MQ=255)                                                                      cGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGa                                                                  <  1:229634/140‑1 (MQ=255)                                                                        gttgttCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATg                                                                >  1:579152/1‑140 (MQ=255)                                                                        gttgttCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATg                                                                >  1:165611/1‑140 (MQ=255)                                                                         ttgttCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATgg                                                               <  1:560861/140‑1 (MQ=255)                                                                             tCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTgag                                                           >  1:106223/1‑140 (MQ=255)                                                                                  gcTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTaa                                                      >  2:117002/1‑140 (MQ=255)                                                                                       cAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGa                                                 <  1:117002/140‑1 (MQ=255)                                                                                                    cAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAAcg                                    >  2:415519/1‑140 (MQ=255)                                                                                                      gCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCTAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAAcgcg                                  <  1:812203/140‑1 (MQ=255)                                                                                                      gCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAAcgcg                                  <  2:16593/140‑1 (MQ=255)                                                                                                      gCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAAcgcg                                  <  1:415519/140‑1 (MQ=255)                                                                                                        aaGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGtt                                <  2:165611/140‑1 (MQ=255)                                                                                                         aGATTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTa                               <  2:234737/140‑1 (MQ=255)                                                                                                           aTTTCGCCGCTTTGTGGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTCGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATc                             <  1:169798/140‑1 (MQ=255)                                                                                                                         tgGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCATCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACg               <  2:729063/140‑1 (MQ=255)                                                                                                                         tgGCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACg               <  2:106223/140‑1 (MQ=255)                                                                                                                           gCTGATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTc             <  1:739309/140‑1 (MQ=255)                                                                                                                              gATACAAGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACg          >  2:438753/1‑140 (MQ=255)                                                                                                                                   aaGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACg     <  1:703939/140‑1 (MQ=255)                                                                                                                                   aaGCCCGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACg     <  1:438753/140‑1 (MQ=255)                                                                                                                                      cccGCCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAg  >  1:615126/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CGGCCCCAGCAGTTGATCAAACAGCCAGACATCGGTAAACACTGCCGAAAACAGTTTGCGATAATCTTCCGGTTGTTCGCCGCTGACAGGTTTGCCATCCAGCAAGATTTCGCCGCTTTGTGGCTGATACAAGCCCGTCAACAACATCGCCAGCGTCGATTTTCCGCTACCGTTGCCGCCAATCAGAAACAGCAGCTCGCCACGTTTGATGGTGAGATTAATCGGACCAACGGAAAACGCGTTATCCTGATAAGCAAACGTCACGTTACGCAG  >  NC_000913/2307371‑2307643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑