Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	417,596	C→T	P152L (CCG→CTG)	phoB →	DNA‑binding transcriptional dual regulator PhoB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	417,596	0	C	T	100.0%	145.9 / NA	44	P152L (CCG→CTG)	phoB	DNA‑binding transcriptional dual regulator PhoB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/23);  total (21/23)

CCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCG  >  NC_000913/417466‑417727                                                                                                                                   |                                                                                                                                     ccGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGAt                                                                                                                             <  2:697216/140‑1 (MQ=255)                  gcgAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTa                                                                                                            <  2:282879/140‑1 (MQ=255)                  gcgAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTa                                                                                                            <  1:320311/140‑1 (MQ=255)                                tAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACttt                                                                                              >  1:630402/1‑140 (MQ=255)                                   tGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACtttttt                                                                                           <  2:148536/140‑1 (MQ=255)                                     cgcCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGGAGAGCTGCTGGGGATGGGGCCGACAGAATTTAAACTGCTGCACcttttttt                                                                                         >  2:302142/1‑138 (MQ=255)                                     cgcCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGGGATGGGGCCGACAGAATTTTAACTGCTGCACTTTTTTAt                                                                                         >  1:482261/1‑140 (MQ=255)                                         gTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACg                                                                                     <  1:118953/140‑1 (MQ=255)                                          tATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGGGAAGAGCTGCTGGGGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGc                                                                                    >  1:358656/1‑140 (MQ=255)                                          tATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGGTGCTGGGGATGGGGCCGACAGAATTTAAAATGCTGCACTTTTTTTTTACGc                                                                                    >  2:15482/1‑140 (MQ=255)                                          tATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGGGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGc                                                                                    >  1:269274/1‑140 (MQ=255)                                          tATTTCGCCAATGGCGGTGGAAGAGGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGGTGGTGGGGATGGGGGCGACAGAATTTTAAATGCCGGACTTTTTTTTTACGc                                                                                    >  2:75047/1‑140 (MQ=255)                                                   agtGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAgc                                                                           <  1:192747/138‑1 (MQ=255)                                                   aaTGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAgc                                                                           <  1:635981/140‑1 (MQ=255)                                                   aaTGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAgc                                                                           <  1:410909/140‑1 (MQ=255)                                                          gTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTa                                                                    <  1:605652/140‑1 (MQ=255)                                                                gagGGGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGGGATGGCGGGCGGAGAGGTGCTGGGGAAGGGGGCCGCAGAAATTTAAATGCTGCCCCTTTTTTTTAAGCATCCCCAGCGCGGGTTCAAccc                                                              >  2:192747/1‑139 (MQ=255)                                                                      aTTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGagca                                                        <  1:12382/140‑1 (MQ=255)                                                                      aTTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGagca                                                        <  1:216906/140‑1 (MQ=255)                                                                           gaTGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑t                                                  <  1:28595/140‑1 (MQ=255)                                                                            aTGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑tt                                                 >  2:745691/1‑140 (MQ=255)                                                                            aTGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑tt                                                 >  2:468247/1‑140 (MQ=255)                                                                                aGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAAc                                             >  2:190231/1‑140 (MQ=255)                                                                                aGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAAc                                             >  2:674648/1‑140 (MQ=255)                                                                                    aTTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACg                                         <  1:621950/140‑1 (MQ=255)                                                                                     ttAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGt                                        >  2:823712/1‑140 (MQ=255)                                                                                       aaGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGAATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCt                                      >  2:192477/1‑140 (MQ=255)                                                                                            tcGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGa                                 >  2:285053/1‑140 (MQ=255)                                                                                            tcGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGa                                 >  2:516746/1‑140 (MQ=255)                                                                                             cGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGaa                                <  1:516746/140‑1 (MQ=255)                                                                                              gACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAAc                               >  2:437538/1‑140 (MQ=255)                                                                                              gACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAAc                               >  2:473713/1‑140 (MQ=255)                                                                                              gACCCGACATCTCACCGAGTGATGGC‑GGCCAAAAGGTGGTGGGGATGGGGCCCACAGAATTTTAAATGCTTCACTTTTTTTTGGCGCAACCTGAGCGCGGGTAAAACCCCCAGCCGGTGTTTAAACACCGTTTGGGGAAc                               >  2:604090/1‑140 (MQ=255)                                                                                                  ccaaaactcCCCGAGGGAAGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAAc                           <  1:604090/134‑1 (MQ=255)                                                                                                    aCATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACgt                         >  1:128146/1‑140 (MQ=255)                                                                                                    aCATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACgt                         <  1:15482/140‑1 (MQ=255)                                                                                                    aCATCTCACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACgt                         <  1:190231/140‑1 (MQ=255)                                                                                                          cACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACGTGTAtgt                   >  1:231597/1‑140 (MQ=255)                                                                                                          cACCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACGTGTAtgt                   >  2:146955/1‑140 (MQ=255)                                                                                                           aCCGAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACGTGTAtgtg                  >  1:593109/1‑140 (MQ=255)                                                                                                              gAGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGAATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACGTGTATGTGGaa               <  1:192477/140‑1 (MQ=255)                                                                                                               aGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACGTGTAtgtg                  <  2:509217/136‑1 (MQ=255)                                                                                                               aGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACGTGTAtgtg                  >  1:509217/1‑136 (MQ=255)                                                                                                               aGTGATGGCGGGCGAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAg              >  1:776960/1‑140 (MQ=255)                                                                                                                       cgggcgAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGTAGCTG‑TTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACg      >  1:738935/1‑140 (MQ=255)                                                                                                                        gggcgAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACgg     <  2:776960/140‑1 (MQ=255)                                                                                                                           cgAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCg  <  2:508798/140‑1 (MQ=255)                                                                                                                           cgAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCg  <  2:630402/140‑1 (MQ=255)                                                                                                                           cgAAGAGCTGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCg  <  1:146955/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                     CCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTGAGATGCAGGGATTAAGTCTCGACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTTAAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTG‑TTAAACCACGTCTGGGGAACTAACGTGTATGTGGAAGACCGCACGGTCG  >  NC_000913/417466‑417727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑