Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,792,661	A→G	E463E (GAA→GAG)	gabD →	NADP(+)‑dependent succinate‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,792,661	0	A	G	100.0%	39.6 / NA	12	E463E (GAA→GAG)	gabD	NADP(+)‑dependent succinate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/7);  total (5/7)

GTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAGTC  >  NC_000913/2792534‑2792771                                                                                                                                |                                                                                                               gTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTAt                                                                                                    >  1:115662/1‑140 (MQ=255)  tGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATg                                                                                                   <  1:524021/140‑1 (MQ=255)        aaGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATGGCATCg                                                                                             <  2:147129/140‑1 (MQ=255)                           ggCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTGTGGCATCGAAGATTACTTAGAAATCaa                                                                          >  2:59339/1‑140 (MQ=255)                               aaGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAatat                                                                      <  2:763882/140‑1 (MQ=255)                                          tACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCgg                                                           <  2:161540/140‑1 (MQ=255)                                            cGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTc                                                         >  1:28769/1‑140 (MQ=255)                                                         aTCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGaa                                            <  1:825674/140‑1 (MQ=255)                                                                      ttattTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAg                               >  2:282549/1‑140 (MQ=255)                                                                            ccAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAACATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATaa                         <  2:115662/140‑1 (MQ=255)                                                                                     tGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAAt                >  2:417799/1‑140 (MQ=255)                                                                                                   ggcATCAAAGCCTCGGGTCTGGGTCGTGAGGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAGTc  <  1:282549/140‑1 (MQ=255)                                                                                                                                |                                                                                                               GTGATTTAAGCCGCGTCTTCCGCGTGGGCGAAGCGCTGGAGTACGGCATCGTCGGCATCAATACCGGCATTATTTCCAATGAAGTGGCCCCGTTCGGCGGCATCAAAGCCTCGGGTCTGGGTCGTGAAGGTTCGAAGTATGGCATCGAAGATTACTTAGAAATCAAATATATGTGCATCGGTCTTTAACTGGAGAATGCGAATGAACAGCAATAAAGAGTTAATGCAGCGCCGCAGTC  >  NC_000913/2792534‑2792771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑