Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,798,154	A→G	R114R (CGT→CGC)	stpA ←	DNA‑binding transcriptional repressor StpA with RNA chaperone activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,798,154	0	A	G	100.0%	143.1 / NA	39	R114R (CGT→CGC)	stpA	DNA‑binding transcriptional repressor StpA with RNA chaperone activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/19);  total (20/19)

AGATGCCGTGGAACCAACGAGCTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGTT  >  NC_000913/2798019‑2798285                                                                                                                                        |                                                                                                                                    aGATGCCGTGGAACCAACGAGCTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGc                                                                                                                                 >  2:554917/1‑140 (MQ=255) aGATGCCGTGGAACCAACGAGCTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGc                                                                                                                                 >  1:757983/1‑140 (MQ=255)  gATGCCGTGGAACCAACGAGCTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGcc                                                                                                                                <  2:113731/140‑1 (MQ=255)    tGCCGTGGAACCAACGAGCTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCt                                                                                                                              >  2:270023/1‑140 (MQ=255)       cGTGGAACCAACGAGCTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGAc                                                                                                                           <  2:757983/140‑1 (MQ=255)            aaCCAACGAGCTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTc                                                                                                                      >  2:205687/1‑140 (MQ=255)             aCCAACGAGCTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTcc                                                                                                                     <  1:205687/140‑1 (MQ=255)                      cTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAg                                                                                                            >  2:823868/1‑140 (MQ=255)                        tGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGtt                                                                                                          <  1:577070/140‑1 (MQ=255)                         gagaAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGttt                                                                                                         <  2:391991/140‑1 (MQ=255)                           gaAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCa                                                                                                       >  2:561352/1‑140 (MQ=255)                           gaAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCa                                                                                                       <  2:18802/140‑1 (MQ=255)                                gACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGtt                                                                                                  <  2:367722/140‑1 (MQ=255)                                 aCGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTa                                                                                                 >  1:547246/1‑140 (MQ=255)                                            gcCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGaa                                                                                      >  2:251319/1‑140 (MQ=255)                                               cTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAAttt                                                                                   >  1:681551/1‑140 (MQ=255)                                                      cGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTc                                                                            >  2:723136/1‑140 (MQ=255)                                                          aTCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCgg                                                                                          <  1:822096/122‑1 (MQ=255)                                                          aTCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCgg                                                                                          >  2:822096/1‑122 (MQ=255)                                                            ccGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGa                                                                      <  2:115977/140‑1 (MQ=255)                                                               gCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGAcgc                                                                   >  1:99834/1‑140 (MQ=255)                                                                     gTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTgg                                                             <  1:270023/140‑1 (MQ=255)                                                                       aaTTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCg                                                           <  1:723136/140‑1 (MQ=255)                                                                              tCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTa                                                    >  1:207077/1‑140 (MQ=255)                                                                              tCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTa                                                    >  1:764663/1‑140 (MQ=255)                                                                                aGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTAcc                                                  >  2:591685/1‑140 (MQ=255)                                                                                aGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTAcc                                                  >  2:641062/1‑140 (MQ=255)                                                                                  gAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAg                                                >  1:445363/1‑140 (MQ=255)                                                                                      tcgtcgAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGcgcg                                            <  2:35870/140‑1 (MQ=255)                                                                                      tcgtcgAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGcgcg                                            <  1:641062/140‑1 (MQ=255)                                                                                       cgtcgAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCgt                                           >  1:404727/1‑140 (MQ=255)                                                                                       cgtcgAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCgt                                           <  2:445363/140‑1 (MQ=255)                                                                                                  ttACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTgcagcagc                                <  1:561352/140‑1 (MQ=255)                                                                                                    aCCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGcagcagcag                              <  1:823868/140‑1 (MQ=255)                                                                                                    aCCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGcagcagcag                              <  1:539495/140‑1 (MQ=255)                                                                                                          tGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTgc                                      >  1:583747/1‑126 (MQ=255)                                                                                                          tGCCAGCGCCTGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTgc                                      <  2:583747/126‑1 (MQ=255)                                                                                                                    tGAGCAATTGGCTTCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATaa              <  1:251319/140‑1 (MQ=255)                                                                                                                                ttCGGTGTGCGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGtt  >  2:360413/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                    AGATGCCGTGGAACCAACGAGCTTGAGAAGCGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGTT  >  NC_000913/2798019‑2798285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑