Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,134	A→G	H122R (CAC→CGC)	mprA →	DNA‑binding transcriptional repressor MprA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,134	0	A	G	100.0%	173.3 / NA	47	H122R (CAC→CGC)	mprA	DNA‑binding transcriptional repressor MprA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/23);  total (24/23)

TGCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAA  >  NC_000913/2811006‑2811267                                                                                                                                 |                                                                                                                                      tgCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTgc                                                                                                                            >  2:571156/1‑140 (MQ=255) tgCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTgc                                                                                                                            >  2:285491/1‑140 (MQ=255) tgCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTgc                                                                                                                            >  1:371952/1‑140 (MQ=255)   ctctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                          <  1:285491/140‑1 (MQ=255)   ctctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                          <  1:304100/140‑1 (MQ=255)   ctctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                          <  1:318940/140‑1 (MQ=255)   ctctTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                          <  1:366551/140‑1 (MQ=255)      ttGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                          <  1:792037/137‑1 (MQ=255)      ttGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTgcgc                                                                                                                          >  2:792037/1‑137 (MQ=255)       tGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGG‑AAAAGGTCGCGAGTTTTTGCGCGAGGt                                                                                                                     <  1:96073/140‑1 (MQ=255)             atcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTac                                                                                                                <  1:571156/140‑1 (MQ=255)             atcCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTac                                                                                                                >  2:228760/1‑140 (MQ=255)               cccGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTacca                                                                                                              >  2:760265/1‑140 (MQ=255)               cccGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTacca                                                                                                              >  2:217997/1‑140 (MQ=255)                  gTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCg                                                                                                           <  1:429914/140‑1 (MQ=255)                   tACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCgc                                                                                                          >  1:65521/1‑140 (MQ=255)                     ccAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCgcag                                                                                                        >  2:409112/1‑140 (MQ=255)                         cgcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATa                                                                                                    >  2:83333/1‑140 (MQ=255)                         cgcgacgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATa                                                                                                    >  1:589175/1‑140 (MQ=255)                             acgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActg                                                                                                >  2:651799/1‑140 (MQ=255)                              cgcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCTGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActgc                                                                                               >  1:724513/1‑140 (MQ=255)                               gcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActgcc                                                                                              <  1:651799/140‑1 (MQ=255)                               gcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActgcc                                                                                              <  1:518345/140‑1 (MQ=255)                               gcgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActgcc                                                                                              <  1:338239/140‑1 (MQ=255)                                cgTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAActgcct                                                                                             >  2:578408/1‑140 (MQ=255)                                    ttGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCAt                                                                                         <  1:760265/140‑1 (MQ=255)                                    ttGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCAt                                                                                         <  2:613822/140‑1 (MQ=255)                                                 ggAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCcg                                                                            >  1:728094/1‑140 (MQ=255)                                                   aaaaaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCcgcg                                                                          <  1:409112/140‑1 (MQ=255)                                                   aaaaaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCcgcg                                                                          <  2:371952/140‑1 (MQ=255)                                                     aaaCGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCt                                                                        <  1:578408/140‑1 (MQ=255)                                                        cgcgGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAg                                                                     <  1:83333/140‑1 (MQ=255)                                                                            tGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGc                                                 <  1:217997/140‑1 (MQ=255)                                                                                           tCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCAccc                                  >  2:350614/1‑140 (MQ=255)                                                                                            cgccgcTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCg                                 >  2:252006/1‑140 (MQ=255)                                                                                              ccgcTGCCTGCATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCa                               <  2:225719/140‑1 (MQ=255)                                                                                                  tgcctgcATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAAtt                           >  2:727656/1‑140 (MQ=255)                                                                                                        cATCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCtctc                     <  1:350614/140‑1 (MQ=255)                                                                                                         aTCTGCAATTAACGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTcc                    >  2:535430/1‑140 (MQ=255)                                                                                                                   aaCGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGa            <  2:612773/138‑1 (MQ=255)                                                                                                                   aaCGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGa            >  1:612773/1‑138 (MQ=255)                                                                                                                   aaCGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGAcc          >  2:552481/1‑140 (MQ=255)                                                                                                                   aaCGGAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGAcc          >  2:815951/1‑140 (MQ=255)                                                                                                                      ggAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGa       <  1:815951/140‑1 (MQ=255)                                                                                                                      ggAAAAAGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGa       <  1:727656/140‑1 (MQ=255)                                                                                                                           aaGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGaa  >  2:692170/1‑140 (MQ=255)                                                                                                                           aaGGTCGCGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGaa  >  1:19324/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                      TGCTCTTGGATCATCCCGTACCAACGCGACGCGTATTGCCGATGAACTGGAAAAACGCGGTTGGATCGAACGTCGTGAAAGCGATAACGATCGCCGCTGCCTGCATCTGCAATTAACGGAAAAAGGTCACGAGTTTTTGCGCGAGGTTTTACCACCGCAGCATAACTGCCTGCATCAACTCTGGTCCGCGCTCAGCACAACAGAAAAAGATCAGCTCGAGCAAATCACCCGCAAATTGCTCTCCCGTCTCGACCAGATGGAA  >  NC_000913/2811006‑2811267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑