Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,905,768 A→G T20A (ACT→GCT)  ygcG → protein YgcG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,905,7680AG98.2% 197.8 / ‑4.1 54T20A (ACT→GCT) ygcGprotein YgcG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (29/24);  minor base T (0/1);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCC  >  NC_000913/2905640‑2905904
                                                                                                                                |                                                                                                                                        
tAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCca                                                                                                                               >  1:126391/1‑140 (MQ=255)
tAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCca                                                                                                                               >  1:170199/1‑140 (MQ=255)
      tAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTTCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTa                                                                                                                         <  2:625599/140‑1 (MQ=255)
       aaCGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTAc                                                                                                                        <  2:725294/140‑1 (MQ=255)
         cGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAg                                                                                                                      >  1:335133/1‑140 (MQ=255)
            atatAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGcaa                                                                                                                   <  2:287334/140‑1 (MQ=255)
             tataAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCGCAATTACAGcaac                                                                                                                  <  2:391085/140‑1 (MQ=255)
             tataAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGcaac                                                                                                                  <  2:772423/140‑1 (MQ=255)
             tataAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGcaac                                                                                                                  <  1:361726/140‑1 (MQ=255)
                  aaTTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGtt                                                                                                             >  1:753036/1‑140 (MQ=255)
                     tACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACg                                                                                                          <  1:362126/140‑1 (MQ=255)
                     tACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACg                                                                                                          <  2:126391/140‑1 (MQ=255)
                     tACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACg                                                                                                          <  1:802218/140‑1 (MQ=255)
                      aCCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACgg                                                                                                         <  1:259118/140‑1 (MQ=255)
                      aCCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACgg                                                                                                         >  1:2440/1‑140 (MQ=255)
                       ccGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGa                                                                                                        >  2:118868/1‑140 (MQ=255)
                         gATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATc                                                                                                      >  2:128388/1‑140 (MQ=255)
                          aTGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCt                                                                                                     >  2:609710/1‑140 (MQ=255)
                                 cgTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACgg                                                                                                         <  1:317228/129‑1 (MQ=255)
                                 cgTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACgg                                                                                                         >  2:317228/1‑129 (MQ=255)
                                              atCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTcac                                                                                 <  2:170199/140‑1 (MQ=255)
                                                 cAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGa                                                                                                <  2:829879/122‑1 (MQ=255)
                                                 cAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGa                                                                                                >  1:829879/1‑122 (MQ=255)
                                                    aTTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACaagaaa                                                                           >  1:699552/1‑140 (MQ=255)
                                                       tAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACaagaaaaga                                                                        <  2:335133/140‑1 (MQ=255)
                                                                   tataATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGc                                                            <  1:118868/140‑1 (MQ=255)
                                                                        tGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAg                                                       >  2:490875/1‑140 (MQ=255)
                                                                        tGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAACTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAg                                                       >  2:424434/1‑140 (MQ=255)
                                                                          cgcTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGtt                                                     >  1:627996/1‑140 (MQ=255)
                                                                           gcTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGca                                                           >  1:165021/1‑133 (MQ=255)
                                                                           gcTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGca                                                           <  2:165021/133‑1 (MQ=255)
                                                                           gcTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTa                                                    >  1:512100/1‑140 (MQ=255)
                                                                            cTATTTCATACTAATGTTCATTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAg                                                   >  2:413404/1‑140 (MQ=255)
                                                                                    tACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTAtt                                           >  2:4126/1‑140 (MQ=255)
                                                                                    tACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTAtt                                           >  2:636476/1‑140 (MQ=255)
                                                                                     aCTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTAttt                                          <  2:2440/140‑1 (MQ=255)
                                                                                         aTGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTAtttt                                         >  1:595115/1‑137 (MQ=255)
                                                                                         aTGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTAtttt                                         <  2:595115/137‑1 (MQ=255)
                                                                                         aTGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCaa                                      >  2:394233/1‑140 (MQ=255)
                                                                                            ttCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCATCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCaataa                                   <  1:288095/140‑1 (MQ=255)
                                                                                              cACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAataata                                 >  2:375502/1‑140 (MQ=255)
                                                                                                   tCGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCaa                            >  1:176936/1‑140 (MQ=255)
                                                                                                    cGTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAg                           >  1:109876/1‑140 (MQ=255)
                                                                                                     gTATGTTCCTTTGTTGCAGCCCAACCAGCTATTGTCCCACAACTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGt                          <  1:424434/140‑1 (MQ=255)
                                                                                                            ccTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGTAAAtt                   <  1:413404/140‑1 (MQ=255)
                                                                                                             cTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTg                  <  1:609710/140‑1 (MQ=255)
                                                                                                             cTTTGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTg                  >  1:35960/1‑140 (MQ=255)
                                                                                                              tttGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGc                 <  1:490875/140‑1 (MQ=255)
                                                                                                              tttGTTGCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGc                 <  1:394233/140‑1 (MQ=255)
                                                                                                               ttgttgCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGcc                >  1:288034/1‑140 (MQ=255)
                                                                                                                    gcggCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTAtt           >  1:483954/4‑140 (MQ=255)
                                                                                                                    gCAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTAtt           >  2:697439/1‑140 (MQ=255)
                                                                                                                     cAGCCCAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTa          >  2:674080/1‑140 (MQ=255)
                                                                                                                        cccAACCAGCTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAAtt       <  2:483954/140‑1 (MQ=255)
                                                                                                                             ccagcTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTcc  >  2:564379/1‑140 (MQ=255)
                                                                                                                                |                                                                                                                                        
TAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCC  >  NC_000913/2905640‑2905904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑