Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	428,722	A→G	D359G (GAC→GGC)	secD →	Sec translocon accessory complex subunit SecD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	428,722	0	A	G	100.0%	199.7 / NA	54	D359G (GAC→GGC)	secD	Sec translocon accessory complex subunit SecD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/23);  total (31/23)

GTGCTGTACAAACGCGTAATTCTGACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATCCAGTCTCGTCTGGGTAAC  >  NC_000913/428586‑428858                                                                                                                                         |                                                                                                                                         gtgCTGTACAAACGCGTAATTCTGACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGgcaa                                                                                                                                       >  2:62063/1‑140 (MQ=255)        aCAAACGCGTAATTCTGACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAGTACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGc                                                                                                                                >  2:705340/1‑140 (MQ=255)         cAAACGCGTAATTCTGACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCa                                                                                                                               <  1:45008/140‑1 (MQ=255)         cAAACGCGTAATTCTGACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCa                                                                                                                               >  1:232538/1‑140 (MQ=255)            aCGCGTAATTCTGACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAAc                                                                                                                            >  1:528214/1‑140 (MQ=255)              gcgTAATTCTGACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCg                                                                                                                          >  1:275226/1‑140 (MQ=255)               cgTAATTCTGACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGa                                                                                                                         <  2:667102/140‑1 (MQ=255)                 tAATTCTGACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATg                                                                                                                       >  1:107251/1‑140 (MQ=255)                   aTTCTGACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGc                                                                                                                     <  2:107251/140‑1 (MQ=255)                   aTTCTGACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGc                                                                                                                     <  2:232538/140‑1 (MQ=255)                      cTGACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAAc                                                                                                                  <  2:540522/140‑1 (MQ=255)                      cTGACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAAc                                                                                                                  <  1:705259/140‑1 (MQ=255)                      cTGACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAAc                                                                                                                  >  2:754337/1‑140 (MQ=255)                           cGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGt                                                                                                             <  1:783184/140‑1 (MQ=255)                              tGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTtg                                                                                                          >  1:244672/1‑140 (MQ=255)                                 ccATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTgg                                                                                                       <  2:656210/140‑1 (MQ=255)                                 ccATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTgg                                                                                                       <  1:754176/140‑1 (MQ=255)                                 ccATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTgg                                                                                                       <  2:244672/140‑1 (MQ=255)                                 ccATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTgg                                                                                                       <  1:240771/140‑1 (MQ=255)                                   atatCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAg                                                                                                     >  2:531849/1‑140 (MQ=255)                                     atCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTa                                                                                                   >  2:155223/1‑140 (MQ=255)                                          cGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAg                                                                                              >  1:54004/1‑140 (MQ=255)                                            aCTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGAc                                                                                            <  1:531849/140‑1 (MQ=255)                                            aCTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGAc                                                                                            >  1:536601/1‑140 (MQ=255)                                             cTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACa                                                                                           <  1:155223/140‑1 (MQ=255)                                             cTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACa                                                                                           <  1:14008/140‑1 (MQ=255)                                                cACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCg                                                                                        >  2:738277/1‑140 (MQ=255)                                                         ccagGACGAGTACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTaagaaag                                                                               <  1:705340/140‑1 (MQ=255)                                                                      aaccaaccGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCg                                                                  <  1:496494/140‑1 (MQ=255)                                                                        ccaaccGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTg                                                                >  2:809146/1‑140 (MQ=255)                                                                           accGCAGGGTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCgg                                                             >  1:114621/1‑140 (MQ=255)                                                                            ccGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGt                                                            >  1:227022/1‑140 (MQ=255)                                                                             cGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGtt                                                           >  1:334118/1‑140 (MQ=255)                                                                                  gTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGt                                                      >  2:696060/1‑140 (MQ=255)                                                                                            cgctcgATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGaa                                            >  1:200597/1‑140 (MQ=255)                                                                                            cgctcgATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGaa                                            >  1:286766/1‑140 (MQ=255)                                                                                            cgctcgATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGaa                                            >  1:391473/1‑140 (MQ=255)                                                                                            cgctcgATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGaa                                            >  2:616774/1‑140 (MQ=255)                                                                                            cgctcgATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGaa                                            >  1:342369/1‑140 (MQ=255)                                                                                                     gcgcTGGTGGTAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGAtt                                   >  1:296028/1‑140 (MQ=255)                                                                                                          ggtggtAACATCATGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACAt                              <  2:209674/140‑1 (MQ=255)                                                                                                                   atcatGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACAt                     <  1:754337/140‑1 (MQ=255)                                                                                                                    tcatGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATc                    <  1:185906/140‑1 (MQ=255)                                                                                                                    tcatGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATc                    <  1:708994/140‑1 (MQ=255)                                                                                                                      atGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATCCa                  >  1:225288/1‑140 (MQ=255)                                                                                                                       tGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATCCAg                 >  2:420022/1‑140 (MQ=255)                                                                                                                       tGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATCCAg                 >  2:377592/1‑140 (MQ=255)                                                                                                                       tGTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATCCAg                 >  2:161183/1‑140 (MQ=255)                                                                                                                        gTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATCCAGt                <  2:286766/140‑1 (MQ=255)                                                                                                                        gTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATCCAGt                <  2:225288/140‑1 (MQ=255)                                                                                                                        gTCTAACTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATCCAGt                >  1:729660/1‑140 (MQ=255)                                                                                                                             aCTTCACTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATCCAGTCTCGt           >  2:623925/1‑140 (MQ=255)                                                                                                                                  aCTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATCCAGTCTCGTCTggg      >  1:709959/1‑140 (MQ=255)                                                                                                                                   cTAAGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATCCAGTCTCGTCTGGGt     >  1:267736/1‑140 (MQ=255)                                                                                                                                      aGGGCAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATCCAGTCTCGTCTGGGTAAc  <  2:342369/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GTGCTGTACAAACGCGTAATTCTGACCGGTGACCATATCACCGACTCCACTTCCAGCCAGGACGAATACAACCAACCGCAGGTTAACATCTCGCTCGATAGCGCTGGTGGTAACATCATGTCTAACTTCACTAAGGACAACATCGGCAAACCGATGGCAACCCTGTTTGTGGAGTACAAAGACAGCGGTAAGAAAGATGCAAATGGTCGTGCGGTTCTGGTGAAACAGGAAGAGGTGATTAACATCGCCAACATCCAGTCTCGTCTGGGTAAC  >  NC_000913/428586‑428858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑