Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,973,345	G→A	A173V (GCC→GTC)	lplT ←	lysophospholipid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,973,345	0	G	A	100.0%	159.9 / NA	47	A173V (GCC→GTC)	lplT	lysophospholipid transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/25);  total (22/25)

TGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCACTAACTTACTACCCGT  >  NC_000913/2973220‑2973478                                                                                                                              |                                                                                                                                      tGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCg                                                                                                                         >  2:380917/1‑140 (MQ=255)  gcgcCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCgg                                                                                                                        <  1:429863/140‑1 (MQ=255)   cgcCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGc                                                                                                                       >  1:223734/1‑140 (MQ=255)            gAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGcgac                                                                                                              <  1:675014/140‑1 (MQ=255)            gAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGcgac                                                                                                              <  1:297836/140‑1 (MQ=255)                tGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAgg                                                                                                          >  1:603301/1‑140 (MQ=255)                    ggCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACAt                                                                                                      >  2:384418/1‑140 (MQ=255)                         tCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAg                                                                                                 <  2:223734/140‑1 (MQ=255)                          cAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGt                                                                                                >  2:24191/1‑140 (MQ=255)                           aGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTc                                                                                               <  1:2274/140‑1 (MQ=255)                           aGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTc                                                                                               <  1:824978/140‑1 (MQ=255)                              aaaCTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTcagc                                                                                            >  1:23253/1‑140 (MQ=255)                                 cTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTcagccag                                                                                         <  2:392182/140‑1 (MQ=255)                                                     aTTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAAc                                                                     >  2:696088/1‑140 (MQ=255)                                                       tCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACaccacc                                                                                <  1:97448/127‑1 (MQ=255)                                                       tCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACaccacc                                                                                >  2:97448/1‑127 (MQ=255)                                                          aGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGc                                                                >  1:286517/1‑140 (MQ=255)                                                                gCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATc                                                          <  1:11934/140‑1 (MQ=255)                                                                gCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATc                                                          >  1:42683/1‑140 (MQ=255)                                                                gCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATc                                                          <  2:23253/140‑1 (MQ=255)                                                                 ccccGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATcg                                                         >  2:404821/1‑140 (MQ=255)                                                                 ccccGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATcg                                                         >  1:174366/1‑140 (MQ=255)                                                                 ccccGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGAACACCACCGGCTACGGAACCGAGCAAAATcg                                                         >  2:726550/1‑140 (MQ=255)                                                                    cGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATcgccg                                                      <  1:108361/140‑1 (MQ=255)                                                                    cGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATcgccg                                                      <  1:384418/140‑1 (MQ=255)                                                                     ggACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATcgccgc                                                     <  1:404821/140‑1 (MQ=255)                                                                     ggACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATcgccgc                                                     <  1:467776/140‑1 (MQ=255)                                                                      gACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCt                                                    <  2:441100/140‑1 (MQ=255)                                                                         gcgcCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATg                                                 <  1:495884/140‑1 (MQ=255)                                                                           gccgccgccAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGt                                               >  1:245929/1‑140 (MQ=255)                                                                           gccgccgccAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGt                                               >  1:91446/1‑140 (MQ=255)                                                                                 gccAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGc                                         <  1:24191/140‑1 (MQ=255)                                                                                  ccAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCt                                        <  1:162251/140‑1 (MQ=255)                                                                                               gTAGATATTGGCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCTGCTATGGTAGAAGCTTCCATTAAACCGt                           <  1:216152/140‑1 (MQ=255)                                                                                                        ggCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCa                  >  2:110166/1‑140 (MQ=255)                                                                                                        ggCAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCa                  <  2:245929/140‑1 (MQ=255)                                                                                                          cAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCttt                    <  1:410049/136‑1 (MQ=255)                                                                                                          cAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCttt                    >  2:410049/1‑136 (MQ=255)                                                                                                          cAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCACt                <  1:417065/140‑1 (MQ=255)                                                                                                          cAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCACt                <  1:380917/140‑1 (MQ=255)                                                                                                          cAACGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCACt                <  1:696088/140‑1 (MQ=255)                                                                                                             cGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCACt                <  1:322009/137‑1 (MQ=255)                                                                                                             cGACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCACt                >  2:322009/1‑137 (MQ=255)                                                                                                              gACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACTGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCACTAACt            >  1:774431/1‑140 (MQ=255)                                                                                                              gACCGCACCACCGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCACTAACt            >  1:12452/1‑140 (MQ=255)                                                                                                                        ccGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCACTAACTTACTAccc    <  1:194940/138‑1 (MQ=255)                                                                                                                        ccGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCACTAACTTACTAccc    >  2:194940/1‑138 (MQ=255)                                                                                                                        ccGTAGACCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCACTAACTTACTACCCGt  >  1:377048/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                      TGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATGTAGATATTGGCAACGACCGCACCACCGTAGGCCAGTGCGCATGCGGCCAGGGCGACGAGGACATGCCAGTCAGCCAGCACACCACCGGCTACGGAACCGAGCAAAATCGCCGCTATGGTAGAAGCTTCCATTAAACCGTTAGCTTTCACTAACTTACTACCCGT  >  NC_000913/2973220‑2973478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑