Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,974,911	C→T	A368A (GCG→GCA)	aas ←	acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,974,911	0	C	T	100.0%	68.5 / NA	21	A368A (GCG→GCA)	aas	acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/12);  total (9/12)

CCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGC  >  NC_000913/2974781‑2975046                                                                                                                                   |                                                                                                                                        ccGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCtcttct                                                                                                                                >  1:65362/1‑140 (MQ=255)        aaaGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGgct                                                                                                                         <  2:624646/140‑1 (MQ=255)              aTCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGtt                                                                                                                   <  2:97046/140‑1 (MQ=255)                  ttGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAAc                                                                                                               <  1:111077/140‑1 (MQ=255)                  ttGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAAc                                                                                                               <  2:193322/140‑1 (MQ=255)                        gtgAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGc                                                                                                         >  1:163159/1‑140 (MQ=255)                         tgAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGcc                                                                                                        >  1:192849/1‑140 (MQ=255)                                     tCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTAAGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATc                                                                                            >  1:16875/1‑140 (MQ=255)                                            aaTCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAAt                                                                                     <  2:712914/140‑1 (MQ=255)                                                 gCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCg                                                                                <  2:108905/140‑1 (MQ=255)                                                 gCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTACCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCg                                                                                <  1:581540/140‑1 (MQ=255)                                                   tCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGaa                                                                              <  1:303899/140‑1 (MQ=255)                                                      aCATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGAt                                                                           <  2:371387/140‑1 (MQ=255)                                                                                        cgCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCtttt                                         >  2:450983/1‑140 (MQ=255)                                                                                          ccTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTaa                                       <  2:300001/140‑1 (MQ=255)                                                                                                       cTTCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAg                          <  1:450983/140‑1 (MQ=255)                                                                                                        ttCAGAACCGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGa                         <  2:784360/140‑1 (MQ=255)                                                                                                                cGGAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAgcgc                 >  2:203841/1‑140 (MQ=255)                                                                                                                  gAGGTAAAAAGGATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCAc               >  1:216217/1‑140 (MQ=255)                                                                                                                             gATCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGtt    >  1:754526/1‑140 (MQ=255)                                                                                                                               tCAGTGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGc  >  1:407337/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        CCGACATAAAGCGATCGTTGGTGGTGAAGTCGGCAATCGTTTTAATCTGCTCGACATTCGCCAGAATGCTTTTATGGCTATGGACGACGCCTTTCGGATGGCCTTCAGAACCGGAGGTAAAAAGGATCAGCGCCTCTTCTTCCGGCTGCTGTTTAACCTGTGCCAGACGCGGCATCAGCAAATGAGCGAAGATCCATACTTTGTCGGCAGTGGTGACATCTGCTTTTAAATCTTCCAGATAGACCCAGCGCACCTGAGTAAGTTGC  >  NC_000913/2974781‑2975046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑