Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,009,716	T→C	V318A (GTG→GCG)	ygeY →	putative peptidase YgeY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,009,716	0	T	C	100.0%	184.7 / NA	50	V318A (GTG→GCG)	ygeY	putative peptidase YgeY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/26);  total (24/26)

AGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGAT  >  NC_000913/3009582‑3009851                                                                                                                                       |                                                                                                                                        aGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGgaag                                                                                                                                    <  1:133686/140‑1 (MQ=255)   gcgcgcTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGgaagaa                                                                                                                                  <  2:652710/140‑1 (MQ=255)    cgcgcTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGaagaag                                                                                                                                 >  2:518076/1‑140 (MQ=255)        cTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCa                                                                                                                             >  1:523420/1‑140 (MQ=255)                        cccTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCa                                                                                                             <  2:353909/140‑1 (MQ=255)                         cctgcctgCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCAc                                                                                                            <  2:555855/140‑1 (MQ=255)                         cctgcctgCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCAc                                                                                                            <  1:518076/140‑1 (MQ=255)                                   gTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGc                                                                                                  <  1:770458/140‑1 (MQ=255)                                   gTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGc                                                                                                  <  2:523420/140‑1 (MQ=255)                                      cAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTa                                                                                               >  2:170191/1‑140 (MQ=255)                                       aGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGc                                                                                                              <  2:493996/124‑1 (MQ=255)                                       aGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGc                                                                                                              >  1:493996/1‑124 (MQ=255)                                       aGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTAc                                                                                              >  1:203862/1‑140 (MQ=255)                                         aaaGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGa                                                                                            >  1:716948/1‑140 (MQ=255)                                              tAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAggg                                                                                        <  2:420360/139‑1 (MQ=255)                                              tAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAggg                                                                                        >  1:420360/1‑139 (MQ=255)                                                aCGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTg                                                                                                       >  2:109580/1‑122 (MQ=255)                                                aCGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTg                                                                                                       <  1:109580/122‑1 (MQ=255)                                                     gttgttTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTgg                                                                                >  2:377430/1‑140 (MQ=255)                                                                gTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCgg                                                                     >  2:173308/1‑140 (MQ=255)                                                                     aCTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGgttgtt                                                                >  1:350869/1‑140 (MQ=255)                                                                      cTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGttgttg                                                               >  2:352202/1‑140 (MQ=255)                                                                      cTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGttgttg                                                               >  2:551427/1‑140 (MQ=255)                                                                         cGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATa                                                            >  2:203043/1‑140 (MQ=255)                                                                           aCCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGa                                                              <  1:756166/136‑1 (MQ=255)                                                                           aCCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGa                                                              >  2:756166/1‑136 (MQ=255)                                                                              gtccgtccTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTgg                                                       <  2:146462/140‑1 (MQ=255)                                                                                ccgtccTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGAc                                                     >  2:751260/1‑140 (MQ=255)                                                                                        gACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCaa                                             >  1:586795/1‑140 (MQ=255)                                                                                          cTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACt                                           <  1:551427/140‑1 (MQ=255)                                                                                          cTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACt                                           <  1:352202/140‑1 (MQ=255)                                                                                             gCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGAcc                                                    >  1:768282/1‑128 (MQ=255)                                                                                             gCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAAc                                        >  2:709799/1‑140 (MQ=255)                                                                                             gCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAAc                                        >  1:118370/1‑140 (MQ=255)                                                                                             gCCTGGTTTACCCAACCGAATGCTACTTCCAGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGAcc                                                    <  2:768282/128‑1 (MQ=255)                                                                                              ccTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACg                                       <  1:493565/140‑1 (MQ=255)                                                                                              ccTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACg                                       <  2:586795/140‑1 (MQ=255)                                                                                                tGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGc                                     <  1:709799/140‑1 (MQ=255)                                                                                                  gTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGt                                   >  1:40512/1‑140 (MQ=255)                                                                                                    ttACCCAACCGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGtat                                 <  2:350869/140‑1 (MQ=255)                                                                                                            ccGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATg                         >  1:278366/1‑140 (MQ=255)                                                                                                            ccGAATGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATg                         >  1:163189/1‑140 (MQ=255)                                                                                                                 tGCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCg                    >  2:340456/1‑140 (MQ=255)                                                                                                                  gCTACTTCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGt                   <  2:203862/140‑1 (MQ=255)                                                                                                                       ttCCCGACCTGGAAAGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACgg              <  1:203043/140‑1 (MQ=255)                                                                                                                                   aaaGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGAt  <  2:278366/140‑1 (MQ=255)                                                                                                                                   aaaGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGAt  <  2:163189/140‑1 (MQ=255)                                                                                                                                   aaaGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGAt  <  1:751260/140‑1 (MQ=255)                                                                                                                                   aaaGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGAt  <  1:377430/140‑1 (MQ=255)                                                                                                                                   aaaGCGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGAt  <  1:170191/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        AGGCGCGCTGGACGAAATCCGCGCCCTGCCTGCAGTACAGAAAGCTAACGCGGTTGTTTCTATGTACAACTACGACCGTCCGTCCTGGACTGGCCTGGTTTACCCAACCGAATGCTACTTCCCGACCTGGAAAGTGGAAGAAGATCACTTCACCGTTAAAGCACTGGTGAATGCCTACGAAGGGCTGTTTGGCAAAGCGCCGGTTGTTGATAAGTGGACCTTCTCAACTAACGGCGTATCTATCATGGGCCGTCACGGCATTCCGGTGAT  >  NC_000913/3009582‑3009851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑