Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,072,812 T→C S294G (AGT→GGT)  epd ← D‑erythrose‑4‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,072,8120TC100.0% 167.5 / NA 46S294G (AGT→GGT) epdD‑erythrose‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/22);  total (24/22)

AAGCAACAGTAGCCATAGCTAACGTCGTGTCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACTGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTC  >  NC_000913/3072680‑3072945
                                                                                                                                    |                                                                                                                                     
aaGCAACAGTAGCCATAGCTAACGTCGTGTCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGa                                                                                                                                >  2:498188/1‑140 (MQ=255)
 aGCAACAGTAGCCATAGCTAACGTCGTGTCGAGCATTCGGTTAGCAAAGCCCCATTAGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGAt                                                                                                                               >  1:702727/1‑140 (MQ=255)
   cAACAGTAGCCATAGCTAACGTCGTGTCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCg                                                                                                                             <  1:34793/140‑1 (MQ=255)
    aaCAGTAGCCATAGCTAACGTCGTGTCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGt                                                                                                                            >  1:820441/1‑140 (MQ=255)
        gTAGCCATAGCTAACGTCGTGTCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGtt                                                                                                                        <  1:352358/140‑1 (MQ=255)
        gTAGCCATAGCTAACGTCATGTCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGtt                                                                                                                        <  1:828576/140‑1 (MQ=255)
                aGCTAACGTCGTGTCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTa                                                                                                                <  1:788647/140‑1 (MQ=255)
                   tAACGTCGTGTCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACag                                                                                                             <  1:258572/140‑1 (MQ=255)
                     aCGTCGTGTCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACagag                                                                                                           >  2:534076/1‑140 (MQ=255)
                     aCGTCGTGTCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACa                                                                                                              <  2:293682/137‑1 (MQ=255)
                     aCGTCGTGTCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACa                                                                                                              >  1:293682/1‑137 (MQ=255)
                      cgtcgtGTCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAgaga                                                                                                          >  1:779091/1‑140 (MQ=255)
                        tcgtGTCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGAcc                                                                                                        <  2:820441/140‑1 (MQ=255)
                           tgtCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAAc                                                                                                     >  1:651597/1‑140 (MQ=255)
                           tgtCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAAc                                                                                                     >  1:759575/1‑140 (MQ=255)
                           tgtCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACAAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAAc                                                                                                     >  1:714458/1‑140 (MQ=255)
                            gtCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACg                                                                                                    >  1:46289/1‑140 (MQ=255)
                             tCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACgg                                                                                                   <  1:498188/140‑1 (MQ=255)
                               gAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCa                                                                                                 <  1:413065/140‑1 (MQ=255)
                                  cATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAAtt                                                                                              <  2:136454/140‑1 (MQ=255)
                                    ttCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTcc                                                                                            <  1:18633/140‑1 (MQ=255)
                                       ggTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGta                                                                                         <  2:779091/140‑1 (MQ=255)
                                             cAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCa                                                                                   >  2:666859/1‑140 (MQ=255)
                                              aaaGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCaa                                                                                  <  2:434208/140‑1 (MQ=255)
                                                  ccccATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACtat                                                                              >  1:498591/1‑140 (MQ=255)
                                                            tATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAAt                                                                    >  1:21127/1‑140 (MQ=255)
                                                                                ttGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTg                                                <  2:651597/140‑1 (MQ=255)
                                                                                 tGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTgc                                               >  1:708070/1‑140 (MQ=255)
                                                                                 tGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTgc                                               >  2:143104/1‑140 (MQ=255)
                                                                                    tCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTgcagc                                            <  1:217317/140‑1 (MQ=255)
                                                                                     cAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTgcagca                                           <  2:385139/140‑1 (MQ=255)
                                                                                        gtgtGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACa                                        >  1:295773/1‑140 (MQ=255)
                                                                                           tgCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGt                                     >  2:724569/1‑140 (MQ=255)
                                                                                            gcgcCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGtt                                    >  1:195799/1‑140 (MQ=255)
                                                                                            gcgcCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGtt                                    <  2:21127/140‑1 (MQ=255)
                                                                                             cgcCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTg                                   <  2:407755/140‑1 (MQ=255)
                                                                                             cgcCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTg                                   >  1:414434/1‑140 (MQ=255)
                                                                                             cgcCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTGGTGCTGCTTTTTGCAGCAACAGGTTg                                   >  1:579791/1‑140 (MQ=255)
                                                                                              gcCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGa                                  <  2:498591/140‑1 (MQ=255)
                                                                                                  cTGACCCGGGTTTGGGTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTc                              >  2:499807/1‑140 (MQ=255)
                                                                                                               gggTGCCATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACa                 <  2:759575/140‑1 (MQ=255)
                                                                                                                     cATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTttc           >  2:23287/1‑140 (MQ=255)
                                                                                                                     cATCGACAATGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTttc           >  1:717049/1‑140 (MQ=255)
                                                                                                                             aTGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGt   <  1:534076/140‑1 (MQ=255)
                                                                                                                             aTGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGt   <  1:499807/140‑1 (MQ=255)
                                                                                                                              tGGCACCGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTc  >  2:618040/1‑140 (MQ=255)
                                                                                                                                    |                                                                                                                                     
AAGCAACAGTAGCCATAGCTAACGTCGTGTCGAGCATTCGGTTAGCAAAGCCCCATTCGTTATCGCACCAGACCAACGTTTTGATCAGGTGTGCGCCACTGACCCGGGTTTGGGTGCCATCGACAATGGCACTGTGCGGATCGTGGTTAAAATCTACAGAGACCAACGGCAATTCCGTATAGTCAACTATACCATGAAATGCACCTTGTGCTGCTTTTTGCAGCAACAGGTTGACTTCATTGGCTTTTACAGGTTTCTTCACCGTC  >  NC_000913/3072680‑3072945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑