Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,097,936	G→A	G224R (GGG→AGG)	yggW →	putative coproporphyrinogen‑III oxidase‑like protein YggW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,097,936	0	G	A	100.0%	148.4 / NA	42	G224R (GGG→AGG)	yggW	putative coproporphyrinogen‑III oxidase‑like protein YggW
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/23);  total (19/23)

CGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACG  >  NC_000913/3097800‑3098071                                                                                                                                         |                                                                                                                                        cGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGc                                                                                                                                      <  2:693049/140‑1 (MQ=255)   aTCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCAt                                                                                                                                    <  2:554379/140‑1 (MQ=255)   aTCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCAt                                                                                                                                    <  2:811525/140‑1 (MQ=255)    tCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATc                                                                                                                                   <  1:141747/140‑1 (MQ=255)        cGCCAGGCCATTGACCTGAATCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGtt                                                                                                                               <  1:632419/140‑1 (MQ=255)            gggCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGttatta                                                                                                                           <  2:197868/139‑1 (MQ=255)              gCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAAc                                                                                                                         >  1:489690/1‑140 (MQ=255)              gCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAAc                                                                                                                         >  1:622502/1‑140 (MQ=255)                      aCTGAATCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCgg                                                                                                                 <  2:347846/140‑1 (MQ=255)                              cgccgcATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAg                                                                                                         <  1:761860/140‑1 (MQ=255)                               gccgcATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGc                                                                                                        >  2:513600/1‑140 (MQ=255)                                 cgcATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCaa                                                                                                      >  1:79320/1‑140 (MQ=255)                                    aTCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAatat                                                                                                   >  1:440993/1‑140 (MQ=255)                                               tATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATAAGCAATATGAAACTTCCGc                                                                                        <  2:489690/140‑1 (MQ=255)                                                aTCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCt                                                                                       >  1:685020/1‑140 (MQ=255)                                                 tCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCtt                                                                                      >  2:619288/1‑140 (MQ=255)                                                   aaCTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTAc                                                                                    <  1:744614/140‑1 (MQ=255)                                                      tGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGcc                                                                                 <  2:98594/140‑1 (MQ=255)                                                        aCCATCGAACCCAATACGCTGTTTGGTTCGCGACTACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCaa                                                                               >  1:3590/1‑140 (MQ=255)                                                         ccATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCaaa                                                                              <  1:619288/140‑1 (MQ=255)                                                             cGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCg                                                                          >  2:482770/1‑140 (MQ=255)                                                                   cAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATc                                                                    <  1:306855/140‑1 (MQ=255)                                                                           tGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAg                                                            >  2:520575/1‑140 (MQ=255)                                                                            gTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGc                                                           >  2:224173/1‑140 (MQ=255)                                                                             tttGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTAt                                                                     >  1:818848/1‑129 (MQ=255)                                                                             tttGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTAt                                                                     <  2:818848/129‑1 (MQ=255)                                                                             tttGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGca                                                          <  1:482770/140‑1 (MQ=255)                                                                               tGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGcaca                                                        <  2:685020/140‑1 (MQ=255)                                                                                   tCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAAtct                                                    >  2:811549/1‑140 (MQ=255)                                                                                       gACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAac                                                >  2:223923/1‑140 (MQ=255)                                                                                                      cGGACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGt                                 <  1:520575/140‑1 (MQ=255)                                                                                                        gACGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGa                               <  2:79320/140‑1 (MQ=255)                                                                                                         aCGATGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGAc                              <  1:224173/140‑1 (MQ=255)                                                                                                             tGACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACa                          <  1:811549/140‑1 (MQ=255)                                                                                                              gACGCGTTGTGGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACAt                         <  2:622502/140‑1 (MQ=255)                                                                                                                       tgGGATATATTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGATTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTgg                >  2:188439/1‑140 (MQ=255)                                                                                                                               atTCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTgcggcg        >  2:64273/1‑140 (MQ=255)                                                                                                                                 tCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGca      >  1:576502/1‑140 (MQ=255)                                                                                                                                 tCGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGca      >  2:818362/1‑140 (MQ=255)                                                                                                                                  cGAACAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGcac     <  1:64273/140‑1 (MQ=255)                                                                                                                                    aaCAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCacac   >  2:723057/1‑140 (MQ=255)                                                                                                                                     aCAGAGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACg  <  2:440993/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CGATCTACGCCAGGCCATTGAACTGAATCCGCCGCATCTTTCCTGGTATCAACTGACCATCGAACCCAATACGCTGTTTGGTTCGCGACCACCGGTGCTGCCGGACGATGACGCGTTGTGGGATATATTCGAACAGGGGCATCAGTTATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACG  >  NC_000913/3097800‑3098071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑