Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	514,566	G→A	G98G (GGC→GGT)	ybbJ ←	NfeD‑like family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	514,566	0	G	A	100.0%	85.4 / NA	25	G98G (GGC→GGT)	ybbJ	NfeD‑like family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/10);  total (15/10)

ATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAG  >  NC_000913/514432‑514673                                                                                                                                       |                                                                                                            aTCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAAt                                                                                                        >  2:1339/1‑140 (MQ=255)  tCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATc                                                                                                       >  1:769808/1‑140 (MQ=255)                aTGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCcgcg                                                                                         <  1:235327/140‑1 (MQ=255)                 tGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCgcgc                                                                                        <  2:379440/140‑1 (MQ=255)                           aTGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTAAAt                                                                              <  2:544567/140‑1 (MQ=255)                             gCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTaa                                                                                >  1:163411/1‑136 (MQ=255)                             gCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTaa                                                                                <  2:163411/136‑1 (MQ=255)                             gCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTAAATGa                                                                            >  1:574747/1‑140 (MQ=255)                               gTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACt                                                                          <  2:574747/140‑1 (MQ=255)                                tACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTaa                                                                                >  1:197102/1‑133 (MQ=255)                                tACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTaa                                                                                <  2:197102/133‑1 (MQ=255)                                   cTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCg                                                                      <  1:1339/140‑1 (MQ=255)                                   cTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCg                                                                      <  2:763053/140‑1 (MQ=255)                                     gCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCt                                                                    <  2:774465/140‑1 (MQ=255)                                       aCCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTaa                                                                                <  1:649031/126‑1 (MQ=255)                                       aCCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTaa                                                                                >  2:649031/1‑126 (MQ=255)                                           agaTCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCtt                                                              >  1:758040/1‑140 (MQ=255)                                           agaTCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCtt                                                              >  2:795284/1‑140 (MQ=255)                                            gaTCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCttt                                                             >  1:412929/1‑140 (MQ=255)                                                cTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGtt                                                         >  1:139507/1‑140 (MQ=255)                                                      gccgcTGACAGGCCATAAACTGTCACCGACGCGCATATGCCCGCGCCCGTTGACCAGCGGAGATCCCACCAAAAAACGCCGACCAACCAGCTGCCCCCCGCGCTGTTTAAAATGACTATCGTTGTTTTTTTGTCCgcgcc                                                   <  2:313438/138‑2 (MQ=255)                                                             aCAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACccgccg                                            >  2:797758/1‑140 (MQ=255)                                                                            tCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTcca                             >  1:643088/1‑140 (MQ=255)                                                                                      gcATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAg                   >  1:822135/1‑140 (MQ=255)                                                                                      gcATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAg                   >  1:101680/1‑140 (MQ=255)                                                                                               cgcgCCCGTCGCCCGGGGGAGATCCCACCAAAAACCGGCGACAAATCGGCTGCGCCCCGCGCTGTTTAAAATGATTTCCGCTGTTTTTTTGTCCCCCCCCCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGagc          <  2:643088/140‑1 (MQ=255)                                                                                                       ttGACCAGCGGAGATTCCAGCACAAAACGTCGACCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAg  >  1:830700/1‑140 (MQ=255)                                                                                                                                       |                                                                                                            ATCCCTTCTATCGCAATGACTTCAACATGCGTACCTGCACCGAGATCCTCGCTGGCGCTGACAGGCCATGAACTGTCACCGACGCGCATATGACCGCGCCCGTTGACCAGCGGAGATTCCAGCACAAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAG  >  NC_000913/514432‑514673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑