Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,180,822	G→A	W134* (TGG→TGA)	ygiC →	putative enzyme with ATPase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,180,822	0	G	A	100.0%	155.6 / NA	45	W134* (TGG→TGA)	ygiC	putative enzyme with ATPase activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/17);  total (28/17)

CATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGGCTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCATCTCACCTGCTGT  >  NC_000913/3180686‑3180957                                                                                                                                         |                                                                                                                                        cATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGGTCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCATCGTCACTATACGAGGCGGCGTTCTTTCAGTGGATctgactg                                                                                                                                      <  2:510304/140‑1 (MQ=255)  aTGGCTGACGCACCAGCCATCGTTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATctgactgg                                                                                                                                     <  1:586675/140‑1 (MQ=255)       tGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGAt                                                                                                                                <  2:612599/140‑1 (MQ=255)         aCGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCa                                                                                                                              >  1:66452/1‑140 (MQ=255)          cgtaCCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAg                                                                                                                             >  1:542444/4‑140 (MQ=255)               cAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTaa                                                                                                                        <  1:691494/140‑1 (MQ=255)                                         tCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCg                                                                                              <  2:745264/140‑1 (MQ=255)                                         tCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCg                                                                                              <  2:260892/140‑1 (MQ=255)                                           tGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGAc                                                                                            <  2:542444/140‑1 (MQ=255)                                             gCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCa                                                                                          <  1:477/140‑1 (MQ=255)                                              cGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTg                                                                                                           >  2:796350/1‑122 (MQ=255)                                              cGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTg                                                                                                           <  1:796350/122‑1 (MQ=255)                                               gTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGt                                                                                        >  2:399250/1‑140 (MQ=255)                                                       gCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGt                                                                                >  1:61655/1‑140 (MQ=255)                                                         aCTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCt                                                                              <  2:696711/140‑1 (MQ=255)                                                             gTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCaa                                                                          >  1:65658/1‑140 (MQ=255)                                                             gTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCaa                                                                          >  1:6177/1‑140 (MQ=255)                                                                     aaaCTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACt                                                                  >  2:686735/1‑140 (MQ=255)                                                                       aCTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTga                                                                >  2:782095/1‑140 (MQ=255)                                                                       aCTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTga                                                                >  1:737747/1‑140 (MQ=255)                                                                              gAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGatcgatcg                                                         <  2:367336/140‑1 (MQ=255)                                                                                aaaTAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGatcgatcg                                                         >  1:444777/1‑138 (MQ=255)                                                                                aaaTAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGatcgatcg                                                         <  2:444777/138‑1 (MQ=255)                                                                                   tAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCg                                                    >  2:605872/1‑140 (MQ=255)                                                                                     aCGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGtt                                                  >  1:250221/1‑140 (MQ=255)                                                                                       gCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGa                                                <  1:609832/140‑1 (MQ=255)                                                                                        ccGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAg                                               <  2:250221/140‑1 (MQ=255)                                                                                           aTACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTg                                            >  1:263234/1‑140 (MQ=255)                                                                                                      tCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGc                                              <  2:126809/127‑1 (MQ=255)                                                                                                      tCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGc                                              >  1:126809/1‑127 (MQ=255)                                                                                                        aCTATACGAGGCGGCGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATg                               >  2:447746/1‑140 (MQ=255)                                                                                                                 ggcggcGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGt                      >  2:151462/1‑140 (MQ=255)                                                                                                                 ggcggcGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGt                      >  2:575995/1‑140 (MQ=255)                                                                                                                 ggcggcGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGt                      <  2:6177/140‑1 (MQ=255)                                                                                                                 ggcggcGTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGt                      >  1:726173/1‑140 (MQ=255)                                                                                                                 ggcggcGTTCTTTCAGGGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGAAGCTACCAGTTTAACAGTCTGCAAGAAAAACTGATCTATCGCTTCGTTGACCTGCGTGAAAAGTATCGCTTCCAGt                      >  2:590128/1‑140 (MQ=255)                                                                                                                  gcggcgTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGtt                     >  2:124727/1‑140 (MQ=255)                                                                                                                   cggcgTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTtg                    <  2:65658/140‑1 (MQ=255)                                                                                                                   cggcgTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTtg                    <  2:726173/140‑1 (MQ=255)                                                                                                                    ggcgTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTTACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGAACGCTTCGGTGAGCAGCGTGAACAGTATGGCTTCCAGTtgc                   >  2:733093/1‑140 (MQ=255)                                                                                                                    ggcgTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTtgc                   >  1:18469/1‑140 (MQ=255)                                                                                                                    ggcgTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGACTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTtgc                   >  2:453922/1‑140 (MQ=255)                                                                                                                      cgTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTtgctg                 >  2:661415/1‑140 (MQ=255)                                                                                                                      cgTTCTTTCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTtgctg                 >  1:685796/1‑140 (MQ=255)                                                                                                                             tCAGTGGATCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCATCTCa          >  2:86460/1‑140 (MQ=255)                                                                                                                                    aTCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCATCTCACctgctg   <  2:685796/140‑1 (MQ=255)                                                                                                                                     tCTGACTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCATCTCACCTGCTGt  >  1:406236/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CATGGCTGACGCACCAGCCATCGCTTTATTCGCGTCTTGATCTGGCGTGGGATGGCACTGGTGAACCTAAACTTCTGGAAAATAACGCCGATACGCCAACGTCACTATACGAGGCGGCGTTCTTTCAGTGGATCTGGCTGGAAGATCAGCTTAACGCCGGTAACTTGCCGGAGGGCAGCGACCAGTTTAACAGTCTGCAAGAAAAACTGATCGATCGCTTCGTTGAGCTGCGTGAACAGTATGGCTTCCAGTTGCTGCATCTCACCTGCTGT  >  NC_000913/3180686‑3180957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑