Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,351,634	T→C	K464K (AAA→AAG)	arcB ←	sensory histidine kinase ArcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,351,634	0	T	C	100.0%	131.7 / NA	41	K464K (AAA→AAG)	arcB	sensory histidine kinase ArcB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/24);  total (17/24)

CGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGTAGCGCACGCGCACGG  >  NC_000913/3351505‑3351767                                                                                                                                  |                                                                                                                                      cGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCAt                                                                                                                             <  2:437738/140‑1 (MQ=255) cGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCAt                                                                                                                             >  2:143767/1‑140 (MQ=255)  gACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATg                                                                                                                            >  2:814097/1‑140 (MQ=255)  gACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATg                                                                                                                            >  2:755538/1‑140 (MQ=255)  gACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATg                                                                                                                            >  2:289954/1‑140 (MQ=255)    cGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGAc                                                                                                                          <  1:814097/140‑1 (MQ=255)    cGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGAc                                                                                                                          <  1:426940/140‑1 (MQ=255)             gATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTACCCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATCCCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCa                                                                                                                 <  2:795172/140‑1 (MQ=255)             gATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCa                                                                                                                 <  1:95508/140‑1 (MQ=255)             gATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCa                                                                                                                 <  2:74842/140‑1 (MQ=255)             gATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCa                                                                                                                 <  1:234344/140‑1 (MQ=255)             gATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCa                                                                                                                 <  1:289954/140‑1 (MQ=255)               tCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCAcc                                                                                                               <  1:314185/140‑1 (MQ=255)                 gTCAACTTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATCCCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTg                                                                                                             <  2:551605/140‑1 (MQ=255)                 gTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGcc                                                                                                                               <  2:658014/122‑1 (MQ=255)                 gTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGcc                                                                                                                               >  1:658014/1‑122 (MQ=255)                 gTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTg                                                                                                             >  1:585768/1‑140 (MQ=255)                 gTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTg                                                                                                             >  2:273667/1‑140 (MQ=255)                        tAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATAc                                                                                                      >  1:312510/1‑140 (MQ=255)                               tGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGa                                                                                               <  1:143767/140‑1 (MQ=255)                                gAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGaa                                                                                              >  1:730042/1‑140 (MQ=255)                                       tGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTa                                                                                       >  1:414541/1‑140 (MQ=255)                                         cccTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATc                                                                                     <  1:755538/140‑1 (MQ=255)                                                cGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGa                                                                                               <  1:314958/123‑1 (MQ=255)                                                cGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGa                                                                                               >  2:314958/1‑123 (MQ=255)                                                cGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCa                                                                              <  1:273667/140‑1 (MQ=255)                                                     gTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTg                                                                         <  1:543861/140‑1 (MQ=255)                                                                    ccgccCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATAcc                                                          >  2:423673/1‑140 (MQ=255)                                                                       cccATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCaga                                                       >  1:797465/1‑140 (MQ=255)                                                                        ccATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCagag                                                      <  1:423673/140‑1 (MQ=255)                                                                          atatTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTc                                                    <  2:312510/140‑1 (MQ=255)                                                                             tttttCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTc                                                 <  2:797465/140‑1 (MQ=255)                                                                                      aGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCTCTTCaaa                                        <  2:585768/140‑1 (MQ=255)                                                                                          gacgaGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATgc                                    <  2:197763/140‑1 (MQ=255)                                                                                                aaaCGGCCAGACCAATCCCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCata                              <  2:552078/140‑1 (MQ=255)                                                                                                         gACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCa                     >  2:30269/1‑140 (MQ=255)                                                                                                                    gtgccggtgGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGTAgcgcac          >  1:502543/1‑140 (MQ=255)                                                                                                                      gccggtgGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGTAgcgcacgc        <  2:730042/140‑1 (MQ=255)                                                                                                                       ccggtgGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCCGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGTAgcgcacgcg       <  1:649581/140‑1 (MQ=255)                                                                                                                         ggtgGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGTAgcgcacgcgca     >  2:775881/1‑140 (MQ=255)                                                                                                                          gtgGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGTAgcgcacgcgcac    <  1:54206/140‑1 (MQ=255)                                                                                                                            ggCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGTAGCGCACGCGCACgg  >  1:419810/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                      CGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGTAGCGCACGCGCACGG  >  NC_000913/3351505‑3351767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑