Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,385,829	G→A	intergenic (+28/+28)	yhcN → / ← yhcO	DUF1471 domain‑containing stress‑induced protein YhcN/putative barnase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,385,829	0	G	A	100.0%	154.6 / NA	47	intergenic (+28/+28)	yhcN/yhcO	DUF1471 domain‑containing stress‑induced protein YhcN/putative barnase inhibitor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/22);  total (25/22)

CGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATG  >  NC_000913/3385694‑3385961                                                                                                                                        |                                                                                                                                     cGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTAcccc                                                                                                                                  <  2:589494/140‑1 (MQ=255)         gCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCgg                                                                                                                          <  1:49327/140‑1 (MQ=255)             aaCAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGCTATTACCCCCGGTTCGGGGGc                                                                                                                      >  2:619925/1‑140 (MQ=255)             aaCAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGc                                                                                                                      >  1:732233/1‑140 (MQ=255)                       cGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTgc                                                                                                            >  2:515405/1‑140 (MQ=255)                         gAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTgcgc                                                                                                          <  1:461072/140‑1 (MQ=255)                                aaGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGAc                                                                                                   <  1:508025/140‑1 (MQ=255)                                    cgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAAc                                                                                               >  1:8649/1‑140 (MQ=255)                                                   gATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATg                                                                                <  1:761581/140‑1 (MQ=255)                                                      tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAATGCAAATGccc                                                                             >  1:513663/1‑140 (MQ=255)                                                      tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGccc                                                                             >  2:10589/1‑140 (MQ=255)                                                        cTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCtt                                                                           <  2:542387/140‑1 (MQ=255)                                                         tGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTCTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTc                                                                          <  2:732233/140‑1 (MQ=255)                                                          gAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                         <  2:502511/140‑1 (MQ=255)                                                             gCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGc                                                                      >  2:779880/1‑140 (MQ=255)                                                             gCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGc                                                                      >  1:750980/1‑140 (MQ=255)                                                              cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                     >  1:268389/1‑140 (MQ=255)                                                                  tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTc                                                                 >  1:777642/1‑140 (MQ=255)                                                                   aGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                         <  2:306138/131‑1 (MQ=255)                                                                   aGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                         >  1:306138/1‑131 (MQ=255)                                                                   aGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTcc                                                                >  1:728909/1‑140 (MQ=255)                                                                         gACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGcc                                                          <  1:515405/140‑1 (MQ=255)                                                                            acCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtca                                                       <  2:750980/140‑1 (MQ=255)                                                                               tGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                    <  1:619836/140‑1 (MQ=255)                                                                                ggCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaa                                                   <  2:8649/140‑1 (MQ=255)                                                                                ggCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGC‑TTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa                                                  <  1:181376/140‑1 (MQ=255)                                                                                 gCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaaa                                                  <  1:453490/140‑1 (MQ=255)                                                                                  cACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAc                                                 <  2:512791/140‑1 (MQ=255)                                                                                    cGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                               <  2:390925/140‑1 (MQ=255)                                                                                               aaCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAAcg                                    >  2:153556/1‑140 (MQ=255)                                                                                               aaCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAAcg                                    >  2:704539/1‑140 (MQ=255)                                                                                               aaCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAAcg                                    >  2:322313/1‑140 (MQ=255)                                                                                                    tACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCa                               >  2:636152/1‑140 (MQ=255)                                                                                                    tACAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCa                               >  1:582102/1‑140 (MQ=255)                                                                                                     aCAAATAAACCCTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCaa                              <  1:779880/140‑1 (MQ=255)                                                                                                              cccTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                     >  2:544669/1‑140 (MQ=255)                                                                                                              cccTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                     >  2:775603/1‑140 (MQ=255)                                                                                                              cccTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                     >  2:493620/1‑140 (MQ=255)                                                                                                              cccTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                     >  2:780876/1‑140 (MQ=255)                                                                                                              cccTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                     >  1:144976/1‑140 (MQ=255)                                                                                                              cccTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACAGTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                     >  2:753547/1‑140 (MQ=255)                                                                                                                cTCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAcgc                   <  2:582102/140‑1 (MQ=255)                                                                                                                 tCATCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAcgcg                  <  1:544669/140‑1 (MQ=255)                                                                                                                   aTCGTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtt                >  2:192821/1‑140 (MQ=255)                                                                                                                      gTCTTGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtc             <  1:704539/140‑1 (MQ=255)                                                                                                                         ttGTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctct          <  1:775603/140‑1 (MQ=255)                                                                                                                           gTCCGACGATATTACCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTcc        >  2:792337/1‑140 (MQ=255)                                                                                                                                 cgaTATTACCCCCGGTTCGGGGGCTTTTTTTTGTGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATg  >  2:818631/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     CGTGAAATGCTGAACAAAAAAGCGGAAGAGAAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATG  >  NC_000913/3385694‑3385961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑