Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,794,338	G→A	A118T (GCC→ACC)	rfaD →	ADP‑L‑glycero‑D‑mannoheptose 6‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,794,338	0	G	A	100.0%	240.8 / NA	68	A118T (GCC→ACC)	rfaD	ADP‑L‑glycero‑D‑mannoheptose 6‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/37);  total (31/37)

CGCGTGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGT  >  NC_000913/3794214‑3794474                                                                                                                             |                                                                                                                                         cgcgTGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCt                                                                                                                                    <  2:195983/131‑1 (MQ=255) cgcgTGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCt                                                                                                                                    >  1:195983/1‑131 (MQ=255)           tCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGa                                                                                                                 <  1:581050/140‑1 (MQ=255)                caccGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTa                                                                                                            <  2:65975/140‑1 (MQ=255)                  ccGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTAtt                                                                                                          >  2:374547/1‑140 (MQ=255)                  ccGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTAtt                                                                                                          >  2:447947/1‑140 (MQ=255)                  ccGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTAtt                                                                                                          >  1:782994/1‑140 (MQ=255)                     aGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGaa                                                                                                       >  2:341132/1‑140 (MQ=255)                      gTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAAt                                                                                                      >  2:731117/1‑140 (MQ=255)                           aCGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACAACCTACGGCGGACGCACCTCCGACTTTATTGAATCCcgc                                                                                                 <  1:230796/140‑1 (MQ=255)                                aaGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTa                                                                                            <  1:176814/140‑1 (MQ=255)                                     tatGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGaaa                                                                                       <  2:817297/140‑1 (MQ=255)                                      atgatgGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGaaaa                                                                                      <  1:425482/140‑1 (MQ=255)                                      atgatgGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGaaaa                                                                                      <  1:588320/140‑1 (MQ=255)                                         atgGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAAcc                                                                                   >  2:488470/1‑140 (MQ=255)                                             aTAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTg                                                                               >  2:443154/1‑140 (MQ=255)                                             aTAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTg                                                                               >  2:460872/1‑140 (MQ=255)                                             aTAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTg                                                                               >  1:482612/1‑140 (MQ=255)                                             aTAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTg                                                                               >  2:580015/1‑140 (MQ=255)                                             aTAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACG‑‑GGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGaa                                                                             >  2:81343/1‑140 (MQ=255)                                              tAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGa                                                                              <  1:447947/140‑1 (MQ=255)                                              tAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGa                                                                              <  1:443154/140‑1 (MQ=255)                                              tAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGa                                                                              <  1:472011/140‑1 (MQ=255)                                              tAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGa                                                                              <  1:783802/140‑1 (MQ=255)                                              tAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGa                                                                              <  2:297727/140‑1 (MQ=255)                                              tAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGa                                                                              <  1:496542/140‑1 (MQ=255)                                              tAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGa                                                                              <  1:536347/140‑1 (MQ=255)                                               aacaacTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGaa                                                                             <  2:782994/140‑1 (MQ=255)                                                   acTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTc                                                                         <  1:397879/140‑1 (MQ=255)                                                     tATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTa                                                                       <  1:374547/140‑1 (MQ=255)                                                      aTCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTg                                                                               <  2:188341/131‑1 (MQ=255)                                                      aTCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTg                                                                               >  1:188341/1‑131 (MQ=255)                                                       tCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACg                                                                     >  2:154629/1‑140 (MQ=255)                                                         aaTACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGt                                                                   <  1:357853/140‑1 (MQ=255)                                                          aTACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGtt                                                                  <  2:657829/140‑1 (MQ=255)                                                              tCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTc                                                              >  1:453701/1‑140 (MQ=255)                                                               ccAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCa                                                             >  1:43143/1‑140 (MQ=255)                                                                 aaGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCaaa                                                           >  2:94051/1‑140 (MQ=255)                                                                 aaGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCaaa                                                           >  1:23475/1‑140 (MQ=255)                                                                 aaGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCaaa                                                           >  1:550277/1‑140 (MQ=255)                                                                   ggAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAAt                                                         >  1:228260/1‑140 (MQ=255)                                                                     aGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTc                                                       >  2:42849/1‑140 (MQ=255)                                                                     aGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTc                                                       >  2:443217/1‑140 (MQ=255)                                                                       ctgctgCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCt                                                     >  2:582200/1‑140 (MQ=255)                                                                       ctgctgCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCt                                                     >  2:398717/1‑140 (MQ=255)                                                                        tgctgcACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCt                                                     >  2:489002/1‑139 (MQ=255)                                                                        tgctgcACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCt                                                     <  1:489002/139‑1 (MQ=255)                                                                        tgctgcACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTg                                                    >  1:276931/1‑140 (MQ=255)                                                                        tgctgcACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTg                                                    <  1:488470/140‑1 (MQ=255)                                                                         gctgcACTACTGCCTGGAGCGAGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGt                                                   >  2:588618/1‑140 (MQ=255)                                                                          ctgcACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGtt                                                  <  1:24327/140‑1 (MQ=255)                                                                          ctgcACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGtt                                                  <  1:94051/140‑1 (MQ=255)                                                                          ctgcACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGtt                                                  <  1:443217/140‑1 (MQ=255)                                                                            gcACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTtg                                                <  1:460872/140‑1 (MQ=255)                                                                            gcACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTtg                                                <  1:341132/140‑1 (MQ=255)                                                                                     ccTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATg                                       <  2:474863/140‑1 (MQ=255)                                                                                     ccTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATg                                       <  1:580015/140‑1 (MQ=255)                                                                                         gAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCg                                   >  2:183183/1‑140 (MQ=255)                                                                                               gAAATCCCGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAAt                             <  1:155594/140‑1 (MQ=255)                                                                                                    cccGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATcctgc                        <  2:276931/140‑1 (MQ=255)                                                                                                      cGTTCCTGTACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATcctgcct                      >  2:701041/1‑140 (MQ=255)                                                                                                              tACGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGaa              <  1:582200/140‑1 (MQ=255)                                                                                                               aCGCTTCTTCCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAAc             <  2:396914/140‑1 (MQ=255)                                                                                                                     cttcCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAg       >  1:440072/1‑140 (MQ=255)                                                                                                                     cttcCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAg       <  2:228260/140‑1 (MQ=255)                                                                                                                      ttcCGCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGa      >  2:585228/1‑140 (MQ=255)                                                                                                                          gCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAttgt  <  2:632914/140‑1 (MQ=255)                                                                                                                          gCAACCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAttgt  <  2:482612/140‑1 (MQ=255)                                                                                                                             |                                                                                                                                         CGCGTGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGT  >  NC_000913/3794214‑3794474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑