Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,828,023	A→G	L215L (TTG→CTG)	gltS ←	glutamate:sodium symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,828,023	0	A	G	100.0%	246.4 / NA	66	L215L (TTG→CTG)	gltS	glutamate:sodium symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/36);  total (30/36)

GTTGCTCAGAATCACGCCAACAAACAGTACACAGACGAAGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAACGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCA  >  NC_000913/3827886‑3828157                                                                                                                                          |                                                                                                                                         gTTGCTCAGAATCACGCCAACAAACAGTACACAGACGAAGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCg                                                                                                                                        >  2:278237/1‑140 (MQ=255) gTTGCTCAGAATCACGCCAACAAACAGTACACAGACGAAGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCg                                                                                                                                        >  2:397631/1‑140 (MQ=255)  ttGCTCAGAATCACGCCAACAAACAGTACACAGACGAAGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGa                                                                                                                                       <  2:97113/140‑1 (MQ=255)   tGCTCAGAATCACGCCAACAAACAGTACACAGACGAAGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCTCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAg                                                                                                                                      <  1:723797/140‑1 (MQ=255)   tGCTCAGAATCACGCCAACAAACAGTACACAGACGAAGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAg                                                                                                                                      <  1:384858/140‑1 (MQ=255)       cAGAATCACGCCAACAAACAGTACACAGACGAAGGTCGGCAGTTC‑AAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGAt                                                                                                                                 <  2:209653/140‑1 (MQ=255)        aGAATCACGCCAACAAACAGTACACAGACGAAGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGAt                                                                                                                                 >  2:799140/1‑140 (MQ=255)         gAATCACGCCAACAAACAGTACACAGACGAAGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATc                                                                                                                                <  1:644394/140‑1 (MQ=255)            tCACGCCAACAAACAGTACACAGACGAAGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑g                                                                                                                           >  1:187304/1‑140 (MQ=255)            tCACGCCAACAAACAGTACACAGACGAAGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑g                                                                                                                           <  1:316866/140‑1 (MQ=255)                              cacaGACGAAGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCttt                                                                                                         >  1:372670/1‑140 (MQ=255)                                caGACGAAGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTc                                                                                                       >  2:144853/1‑140 (MQ=255)                                 aGACGAAGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCATGCGCGTCCCACATCCGGCtttt                                                                                                        >  2:299835/1‑140 (MQ=255)                                 aGACGAAGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCa                                                                                                      >  2:612078/1‑140 (MQ=255)                                   aCGAAGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCaaa                                                                                                    >  1:684527/1‑140 (MQ=255)                                   aCGAAGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCaaa                                                                                                    >  2:804261/1‑140 (MQ=255)                                      aaGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGc                                                                                                 >  1:623947/1‑140 (MQ=255)                                      aaGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGc                                                                                                 >  2:182863/1‑140 (MQ=255)                                        ggTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCg                                                                                               >  1:579666/1‑140 (MQ=255)                                             gCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCggg                                                                                          >  1:167025/1‑140 (MQ=255)                                             gCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCggg                                                                                          >  1:266175/1‑140 (MQ=255)                                             gCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCggg                                                                                          >  1:434909/1‑140 (MQ=255)                                              cAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGa                                                                                         <  1:288002/140‑1 (MQ=255)                                                 ttCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACtt                                                                                      >  1:176724/1‑140 (MQ=255)                                                      aaGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTgg                                                                                 <  2:434909/140‑1 (MQ=255)                                                        gCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTc                                                                               <  1:473290/140‑1 (MQ=255)                                                           gTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCGAACGCCGTCGGGACTTCCTGGTCATc                                                                            >  1:579324/1‑140 (MQ=255)                                                           gTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATc                                                                            >  1:540214/1‑140 (MQ=255)                                                           gTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATc                                                                            >  2:90735/1‑140 (MQ=255)                                                                 gccaAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAAt                                                                      <  2:565626/140‑1 (MQ=255)                                                                       aGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGtt                                                                <  2:266175/140‑1 (MQ=255)                                                                         ttGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCg                                                              <  1:487388/140‑1 (MQ=255)                                                                           gcgcAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGc                                                            <  2:558875/140‑1 (MQ=255)                                                                            cgcAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCg                                                           >  1:810630/1‑140 (MQ=255)                                                                            cgcAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCg                                                           >  1:122990/1‑140 (MQ=255)                                                                                  aaTTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGtggtgg                                                     <  2:187304/140‑1 (MQ=255)                                                                                      ttCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAgtg                                                 <  1:12042/140‑1 (MQ=255)                                                                                      ttCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAgtg                                                 <  2:579324/140‑1 (MQ=255)                                                                                      ttCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAgtg                                                 <  1:203798/140‑1 (MQ=255)                                                                                      ttCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAgtg                                                 <  1:182863/140‑1 (MQ=255)                                                                                      ttCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAgtg                                                 <  2:146443/140‑1 (MQ=255)                                                                                      ttCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAgtg                                                 <  1:615026/140‑1 (MQ=255)                                                                                        ccccACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGtt                                               <  2:623947/140‑1 (MQ=255)                                                                                        ccccACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCAGAATACCGTTCGGCGTGGTGGAGTGtt                                               <  2:565329/140‑1 (MQ=255)                                                                                         cccACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGttt                                              <  2:251119/140‑1 (MQ=255)                                                                                              cGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAgtg                                                 <  2:25600/132‑1 (MQ=255)                                                                                              cGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAgtg                                                 >  1:25600/1‑132 (MQ=255)                                                                                                tCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGa                                       >  2:598534/1‑140 (MQ=255)                                                                                                     cAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAgcg                                  <  2:372670/140‑1 (MQ=255)                                                                                                      aGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAgcgc                                 <  2:579666/140‑1 (MQ=255)                                                                                                             aaTCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCg                          <  2:176724/140‑1 (MQ=255)                                                                                                              aTCAGCGCGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCgg                         >  1:308675/1‑140 (MQ=255)                                                                                                                  gcgcGATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCg                     >  2:159681/1‑140 (MQ=255)                                                                                                                   cgcgATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGc                    >  2:445996/1‑140 (MQ=255)                                                                                                                   cgcgATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGc                    >  2:431515/1‑140 (MQ=255)                                                                                                                    gcgATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTTATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGcc                   >  2:584799/1‑140 (MQ=255)                                                                                                                      gATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCaa                 >  1:266705/1‑140 (MQ=255)                                                                                                                      gATAGTTTCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCaa                 <  1:159681/140‑1 (MQ=255)                                                                                                                           tttCAATCAGCACCAGCGAGTTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAg            <  1:804261/140‑1 (MQ=255)                                                                                                                           tttCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAg            <  1:431515/140‑1 (MQ=255)                                                                                                                            ttCAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAgc           <  2:316371/140‑1 (MQ=255)                                                                                                                              cAATCAGCACCATCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCGGTTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAgccg         <  1:278237/140‑1 (MQ=255)                                                                                                                              cAATCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAgccg         <  1:397631/140‑1 (MQ=255)                                                                                                                                aTCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAgccgcc       <  2:540214/140‑1 (MQ=255)                                                                                                                                aTCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAgccgcc       <  1:144853/140‑1 (MQ=255)                                                                                                                                aTCAGCACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAgccgcc       <  1:612078/140‑1 (MQ=255)                                                                                                                                     cACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCa  <  2:530240/140‑1 (MQ=255)                                                                                                                                     cACCAGCGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCa  >  1:530240/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         GTTGCTCAGAATCACGCCAACAAACAGTACACAGACGAAGGTCGGCAGTTCAAAAGCAGTGCCAGCCAAAAGTTGCGCAACAATTTTCCCCACCGTCAGGCAGATAGCAATCAGCGCGATAGTTTCAATCAGCACCAACGAGGTGATCAT‑‑GCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCA  >  NC_000913/3827886‑3828157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑