Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,117,585 Δ1 bp coding (820/1281 nt) wcaK ← putative colanic acid biosynthesis pyruvyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,117,5850A.100.0% 178.3 / NA 43coding (820/1281 nt)wcaKputative colanic acid biosynthesis pyruvyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (24/19);  total (24/19)

TGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACGT  >  NC_000913/2117456‑2117719
                                                                                                                                 |                                                                                                                                      
tGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCga                                                                                                                             >  1:42823/1‑140 (MQ=255)
                 aCGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCACAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGaccacc                                                                                                            <  2:219891/140‑1 (MQ=255)
                  cGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCAccc                                                                                                           >  2:23929/1‑140 (MQ=255)
                       tAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCa                                                                                                      >  1:584130/1‑140 (MQ=255)
                          cGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAg                                                                                                   >  1:439693/1‑140 (MQ=255)
                          cGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAg                                                                                                   >  1:10251/1‑140 (MQ=255)
                          cGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAg                                                                                                   >  1:489485/1‑140 (MQ=255)
                                 gATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCttttt                                                                                            >  1:614833/1‑140 (MQ=255)
                                       tGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACg                                                                                      <  2:614833/140‑1 (MQ=255)
                                        gATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGc                                                                                     <  1:421253/140‑1 (MQ=255)
                                            tgCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGTTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTAATACGCttgt                                                                                 >  1:568467/1‑140 (MQ=255)
                                             gCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCttgtt                                                                                <  2:42823/140‑1 (MQ=255)
                                                ggCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAg                                                                             <  2:542097/140‑1 (MQ=255)
                                                      ggTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGc                                                                       >  1:634567/1‑140 (MQ=255)
                                                       gTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGcc                                                                      <  2:726695/140‑1 (MQ=255)
                                                           aGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCgaga                                                                  >  1:16361/1‑140 (MQ=255)
                                                              gcCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGt                                                               <  2:548341/140‑1 (MQ=255)
                                                                      gCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCaaa                                                       <  2:584130/140‑1 (MQ=255)
                                                                      gCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCaaa                                                       <  1:23929/140‑1 (MQ=255)
                                                                          tcgtcTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGt                                                   >  2:483152/1‑140 (MQ=255)
                                                                            gtcTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGc                                                 >  2:417050/1‑140 (MQ=255)
                                                                                ttGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGt                                             <  2:634567/140‑1 (MQ=255)
                                                                                 tGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCaaa                                                       <  1:74325/129‑1 (MQ=255)
                                                                                 tGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCaaa                                                       >  2:74325/1‑129 (MQ=255)
                                                                                       aGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCa                                      <  1:677381/140‑1 (MQ=255)
                                                                                           gTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGt                                  >  2:329160/1‑140 (MQ=255)
                                                                                            tCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTa                                 >  2:144260/1‑140 (MQ=255)
                                                                                             cAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTcgcg                                        <  1:826503/132‑1 (MQ=255)
                                                                                             cAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTcgcg                                        >  2:826503/1‑132 (MQ=255)
                                                                                             cAATGCCCGTACAGGTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTaa                                >  2:31517/1‑140 (MQ=255)
                                                                                                           gTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTc                  >  2:370396/1‑140 (MQ=255)
                                                                                                           gTGGAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTAATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTc                  >  2:308798/1‑140 (MQ=255)
                                                                                                             ggAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTc                  <  1:84980/138‑1 (MQ=255)
                                                                                                             ggAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTc                  >  2:84980/1‑138 (MQ=255)
                                                                                                             ggAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCtg                >  1:776177/1‑140 (MQ=255)
                                                                                                             ggAGAGCGCAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCtg                <  1:483152/140‑1 (MQ=255)
                                                                                                                   cgcgaTCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGc          >  1:812055/5‑140 (MQ=255)
                                                                                                                   cgcAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGc          >  1:488530/1‑140 (MQ=255)
                                                                                                                   cgcAATCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGc          >  2:807707/1‑140 (MQ=255)
                                                                                                                      gaTCACCTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGc       <  2:812055/139‑1 (MQ=255)
                                                                                                                           ccTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACGt  <  2:488530/140‑1 (MQ=255)
                                                                                                                           ccTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACGt  <  1:370396/140‑1 (MQ=255)
                                                                                                                           ccTGAT‑CCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACGt  <  1:329160/140‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                      
TGAGTTCATCCATCACTACGTGGTAACGGGCAGGATCGCTGATGTGCTGGCGCAGGTTGAGCGCCACCATGCGGTCGTCTTTGTTATAGCTGTCAATGCCCGTACAGGTGGAGAGCGCAATCACCTGATACCCTTCATCGAGAATGCGATTGACCACCCCGGCAAAGGCTTTTTCATACGCTTGTTGAGTGGTGCCGAGACGTTTGTCAAACGGTGCCAGTTCGCGCAGGGTAATGGCCACCGTTTTCTGTTGTGCGGCAACGT  >  NC_000913/2117456‑2117719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑