Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,230,404	(A)7→6	intergenic (‑19/‑220)	dusC ← / → yohJ	tRNA‑dihydrouridine(16) synthase/PF03788 family membrane protein YohJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,230,398	0	A	.	100.0%	182.8 / NA	44	intergenic (‑13/‑226)	dusC/yohJ	tRNA‑dihydrouridine(16) synthase/PF03788 family membrane protein YohJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (22/22);  total (22/22)

AGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGC  >  NC_000913/2230264‑2230533                                                                                                                                       |                                                                                                                                        agcagTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCaaaaaa                                                                                                                                    >  2:215248/1‑140 (MQ=255)  gcagTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCaaaaaat                                                                                                                                   <  2:649389/140‑2 (MQ=255)             accacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGACAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATg                                                                                                                       >  2:372592/1‑140 (MQ=255)                 cgcgGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAg                                                                                                                   >  2:75420/1‑140 (MQ=255)                   cgGACAAACTTGGTTATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAgcg                                                                                                                 <  2:510685/140‑1 (MQ=255)                           cTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGt                                                                                                         <  1:215248/140‑1 (MQ=255)                              ggTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAg                                                                                                      <  2:540927/140‑1 (MQ=255)                              ggTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAg                                                                                                      <  1:418063/140‑1 (MQ=255)                              ggTGATGCACAGATCGTAGTCGTTAACTTCGGACAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAg                                                                                                      <  1:372592/140‑1 (MQ=255)                                tGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATg                                                                                                           <  2:283337/133‑1 (MQ=255)                                tGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATg                                                                                                           >  1:283337/1‑133 (MQ=255)                                     cacaGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTa                                                                                               >  2:352581/1‑140 (MQ=255)                                       caGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTaaa                                                                                             <  2:9456/140‑1 (MQ=255)                                           tCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTc                                                                                         <  1:126139/140‑1 (MQ=255)                                            cGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCt                                                                                        >  2:417210/1‑140 (MQ=255)                                               aGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCa                                                                                     >  2:48716/1‑140 (MQ=255)                                                    ttAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTa                                                                                               <  1:735579/125‑1 (MQ=255)                                                    ttAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTa                                                                                               >  2:735579/1‑125 (MQ=255)                                                      aaCTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAggg                                                                              >  1:434102/1‑140 (MQ=255)                                                      aaCTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAggg                                                                              >  2:459119/1‑140 (MQ=255)                                                            ggTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgct                                                                        <  1:352581/140‑1 (MQ=255)                                                                          gCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                          <  2:320050/140‑1 (MQ=255)                                                                          gCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                          <  1:757472/140‑1 (MQ=255)                                                                          gCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                          <  1:215350/140‑1 (MQ=255)                                                                           cACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATccc                                                         <  1:459119/140‑1 (MQ=255)                                                                            aCCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCct                                                        >  2:222532/1‑140 (MQ=255)                                                                            aCCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCct                                                        <  1:417210/140‑1 (MQ=255)                                                                                    gTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGc                                                >  2:29960/1‑140 (MQ=255)                                                                                        aGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                          >  2:182379/1‑126 (MQ=255)                                                                                        aGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                          <  1:182379/126‑1 (MQ=255)                                                                                        aGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCctctct                                                    >  2:187815/1‑132 (MQ=255)                                                                                        aGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCctctct                                                    <  1:187815/132‑1 (MQ=255)                                                                                        aGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGa                                            >  2:221004/1‑140 (MQ=255)                                                                                                 tccATCGGTGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCt                                   <  1:29960/140‑1 (MQ=255)                                                                                                         tGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGCTTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGc                           <  2:576660/140‑1 (MQ=255)                                                                                                         tGCCAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGc                           >  1:792125/1‑140 (MQ=255)                                                                                                           ccAGTAACACACGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTg                         >  1:603372/1‑140 (MQ=255)                                                                                                                  cacacGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCt                          >  2:131737/1‑132 (MQ=255)                                                                                                                  cacacGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCt                          <  1:131737/132‑1 (MQ=255)                                                                                                                   acacGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCt                          >  2:44167/1‑131 (MQ=255)                                                                                                                   acacGCATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCt                          <  1:44167/131‑1 (MQ=255)                                                                                                                        cATATCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAAt            <  1:48716/140‑1 (MQ=255)                                                                                                                         atatCATCACCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATg           >  2:614936/1‑140 (MQ=255)                                                                                                                                caCCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGa    >  2:495174/1‑140 (MQ=255)                                                                                                                                 aCCCGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAg   >  2:209799/1‑140 (MQ=255)                                                                                                                                  cccGC‑AAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGc  >  2:110358/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        AGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGC  >  NC_000913/2230264‑2230533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑