Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	342,567	Δ1 bp	intergenic (+60/‑317)	yahJ → / → yahK	putative deaminase with metallo‑dependent hydrolase domain/aldehyde reductase, NADPH‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	342,564	0	G	.	100.0%	144.1 / NA	35	intergenic (+57/‑320)	yahJ/yahK	putative deaminase with metallo‑dependent hydrolase domain/aldehyde reductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (20/15);  total (20/15)

GCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTACAG  >  NC_000913/342427‑342697                                                                                                                                          |                                                                                                                                      gccgAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGTggg                                                                                                                                     >  2:776209/1‑140 (MQ=255)   cgAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGTgggca                                                                                                                                   <  2:482854/140‑3 (MQ=255)     gggCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGTgggcaat                                                                                                                                 <  1:54186/139‑5 (MQ=255)     aGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAAC‑‑GTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCt                                                                                                                              >  1:789974/1‑140 (MQ=255)           tGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAat                                                                                                                          <  1:768622/140‑1 (MQ=255)              cgcgTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATg                                                                                                                       <  2:345539/140‑1 (MQ=255)                       cGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCg                                                                                                              >  2:769382/1‑140 (MQ=255)                                 cGCAACGTTCCATAAAGGGCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATgg                                                                                                    >  2:421532/1‑140 (MQ=255)                                     aCGTTCCATAAAGGGCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACAt                                                                                                <  2:621920/140‑1 (MQ=255)                                     aCGTTCCATAAAGGGCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACAt                                                                                                <  1:421532/140‑1 (MQ=255)                                       gTTCCATAAAGGGCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTa                                                                                              <  1:471971/140‑1 (MQ=255)                                        ttCCATAAAGGGCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTAt                                                                                             >  1:739743/1‑140 (MQ=255)                                        ttCCATAAAGGGCAAC‑‑GTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCt                                                                                           <  2:789974/140‑1 (MQ=255)                                                  ggCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGaa                                                                                   >  1:558059/1‑140 (MQ=255)                                                     aaCTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAatat                                                                                >  1:677058/1‑140 (MQ=255)                                                      aCTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATg                                                                               <  2:360916/140‑1 (MQ=255)                                                       cTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGc                                                                              >  1:134446/1‑140 (MQ=255)                                                        tGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGcc                                                                             <  2:558059/140‑1 (MQ=255)                                                            gtgGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTa                                                                         >  1:132114/1‑140 (MQ=255)                                                                gggAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGa                                                                     >  2:125728/1‑140 (MQ=255)                                                                 ggAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGAc                                                                    <  2:132114/140‑1 (MQ=255)                                                                    gtgtGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGtt                                                                 >  1:244257/1‑140 (MQ=255)                                                                     tgtgGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTAt                                                                        <  1:97186/132‑1 (MQ=255)                                                                     tgtgGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTAt                                                                        >  2:97186/1‑132 (MQ=255)                                                                            ggTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGAt                                                         <  1:125728/140‑1 (MQ=255)                                                                              ttGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATaa                                                       >  2:735825/1‑140 (MQ=255)                                                                                               cGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGaa                                      <  1:735825/140‑1 (MQ=255)                                                                                                         ggTGAATAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGAtcatca                            >  2:578434/1‑140 (MQ=255)                                                                                                             aaTAGTAAGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGttt                        >  1:392122/1‑140 (MQ=255)                                                                                                                   aaGAGATTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGa                  >  1:360247/1‑140 (MQ=255)                                                                                                                      agaTTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGAttt               <  1:769382/140‑1 (MQ=255)                                                                                                                       gaTTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGAttt               >  2:257077/1‑139 (MQ=255)                                                                                                                       gaTTTAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGAttt               <  1:257077/139‑1 (MQ=255)                                                                                                                          ttAAGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGt           >  1:475284/1‑140 (MQ=255)                                                                                                                            aaGCCCCAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCt         >  2:780283/1‑140 (MQ=255)                                                                                                                                  cAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTTCGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTACa   <  2:135217/140‑1 (MQ=255)                                                                                                                                  cAGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTACa   >  1:602120/1‑140 (MQ=255)                                                                                                                                   aGGGAGT‑GGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTACAg  >  1:396355/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      GCCGAGGCGGTGGCGCGTATCTCGCCGAGAACCGCAACGTTCCATAAAGGGCAACTGGTGTGGGGGAGTGTGGCAGGTTGATGCGCATTTGAAACGCCTGCAACGGTGAATAGTAAGAGATTTAAGCCCCAGGGAGTGGGGCAATCTGAATATGAGTGTGTCGGCAGAAATGGACATTATCTTTGAGGAATATGCCCTTATTGACGTTGTGTAGATAACTATTTGTCTACGTGAAAAGATCATCAGTTTTGCCGATTTTAGTCTTTTACAG  >  NC_000913/342427‑342697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑