Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,266,897	(T)5→4	coding (169/939 nt)	tdcA ←	DNA‑binding transcriptional activator TdcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,266,893	0	T	.	100.0%	148.3 / NA	36	coding (173/939 nt)	tdcA	DNA‑binding transcriptional activator TdcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (23/13);  total (23/13)

CCAAATGAGACTTCCACCACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGA  >  NC_000913/3266761‑3267025                                                                                                                                     |                                                                                                                                     ccAAATGAGACTTCCACCACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTAttttccgc                                                                                                                               <  2:492905/140‑5 (MQ=255) ccAAATGAGACTTCCACCACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTAttttccgc                                                                                                                               <  1:403857/140‑5 (MQ=255)  cAAATGAGACTTCCACCACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGca                                                                                                                             <  1:647780/140‑1 (MQ=255)   aaaTGAGACTTCCACCACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGcac                                                                                                                            >  1:500897/1‑140 (MQ=255)       gagaCTTCCACCACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACt                                                                                                                        <  1:277055/140‑1 (MQ=255)               caccacCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCcac                                                                                                                >  2:123923/1‑140 (MQ=255)                cccaccGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCcaca                                                                                                               <  2:61800/139‑1 (MQ=255)                     cGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCaaa                                                                                                          >  2:631217/1‑140 (MQ=255)                       ccTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCGCCACTAATTCCACACCAAaat                                                                                                        >  2:754672/1‑140 (MQ=255)                       ccTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAaat                                                                                                        >  1:119688/1‑140 (MQ=255)                        cTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAaata                                                                                                       <  1:165892/140‑1 (MQ=255)                          cAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAaataat                                                                                                     <  1:95143/140‑1 (MQ=255)                                 cATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAat                                                                                              <  1:754672/140‑1 (MQ=255)                                  aTACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAaata                                                                                                       >  2:694716/1‑130 (MQ=255)                                  aTACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAaata                                                                                                       <  1:694716/130‑1 (MQ=255)                                  aTACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAata                                                                                             >  1:486822/1‑140 (MQ=255)                                  aTACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAata                                                                                             >  1:176988/1‑140 (MQ=255)                                  aTACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAata                                                                                             >  1:115417/1‑140 (MQ=255)                                  aTACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAata                                                                                             >  1:480248/1‑140 (MQ=255)                                    aCCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATc                                                                                           >  2:741243/1‑140 (MQ=255)                                         ttATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTaa                                                                                      >  1:344986/1‑140 (MQ=255)                                          tATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAaata                                                                                                       >  1:671717/1‑122 (MQ=255)                                          tATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAaata                                                                                                       <  2:671717/122‑1 (MQ=255)                                                    aCCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTg                                                                           <  2:344986/140‑1 (MQ=255)                                                    aCCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTg                                                                           <  2:480248/140‑1 (MQ=255)                                                        tatTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACgg                                                                       <  2:471194/140‑1 (MQ=255)                                                          tttttCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGcc                                                                     >  2:675517/1‑140 (MQ=255)                                                            tttcatttcaCGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCgg                                                                   <  1:741243/140‑1 (MQ=255)                                                                      cGGGTAATGGATTCGGAACGGGAGAGTAACAATTTACCGGCAGGTGTTAATGTTACACCGGGA‑TTTTCCGCACCACTAATTCCACTCCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAAc                                                         >  2:548662/1‑140 (MQ=255)                                                                                          ggAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCagccga                                     >  1:415987/1‑140 (MQ=255)                                                                                           gagaGTAACAATTGACCGGAAGTTTTAAATTTTACCCCGGT‑TTTTCCCGCACCATTAATTCCACCCCAAAAAATTCTCCAATATCTTTAATGATTTTACGGACGGCCGGTTGAGTTAACCCTAATTATTTTGCagccgag                                    <  2:631917/140‑1 (MQ=255)                                                                                                     aTTGACCGGCAGGTTTTAATGTTACACGGGTA‑TTTTCCGCACCACTAATCCCACACAAAAATAATCTTAAATATCGTTAATGATTTTACTGACGGCGGGTTGAGTTACCCCTAATTCTTTGGCAGCCGAGCCGATAGAAc                          <  2:119688/140‑1 (MQ=255)                                                                                                     aTTGACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACAAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTACCCCTAATTCTTTTGCAGCCGAGCCGATAGAAc                          <  1:631217/140‑1 (MQ=255)                                                                                                        gACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCAc                       >  2:84243/1‑140 (MQ=255)                                                                                                        gACCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCAc                       >  2:281634/1‑140 (MQ=255)                                                                                                         aCCGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACt                      >  1:33167/1‑140 (MQ=255)                                                                                                          ccGGCAGGTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACtt                     >  2:136120/1‑140 (MQ=255)                                                                                                                ggTGTTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATg               >  1:89641/1‑140 (MQ=255)                                                                                                                  tgtTAATGTTACACCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGAc             <  1:136120/140‑1 (MQ=255)                                                                                                                        tGTTACACCGGT‑TTTTTCCGCCCCACAAATCCCACACAAAAATAATCTTAAATATCGTAAATGATTTTACTGACGGCCGGTTGAGTTACCCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTg       <  2:115417/140‑1 (MQ=255)                                                                                                                            acacCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAg   >  1:27371/1‑140 (MQ=255)                                                                                                                             cacCGGTA‑TTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCTTTAATGATTTTACTGACGGCCGGTTGATTTACCCCTAATTTTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGa  <  2:176988/140‑1 (MQ=255)                                                                                                                              acCGGTA‑TTTTCCGCACCACT‑ATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGaa     <  2:4144/135‑1 (MQ=255)                                                                                                                              acCGGTA‑TTTTCCGCACCACT‑ATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGaa     >  1:4144/1‑135 (MQ=255)                                                                                                                                     |                                                                                                                                     CCAAATGAGACTTCCACCACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGGGTAATGGATTCGGAACGGGAGAGTAACAATTGACCGGCAGGTGTTAATGTTACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGA  >  NC_000913/3266761‑3267025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑