Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	743,934	(A)5→4	coding (342/657 nt)	pxpB →	5‑oxoprolinase component B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	743,930	0	A	.	100.0%	100.3 / NA	25	coding (338/657 nt)	pxpB	5‑oxoprolinase component B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (13/12);  total (13/12)

AGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGC  >  NC_000913/743796‑744057                                                                                                                                       |                                                                                                                                agagCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGaaaaca                                                                                                                            <  2:492492/140‑3 (MQ=255) agagCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGaaaaca                                                                                                                            <  1:41010/140‑3 (MQ=255)   agCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGaaaacagg                                                                                                                          >  2:88660/1‑136 (MQ=255)     cGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGTCGCATTGCGGGTTGAGCG‑AAAACAGgtt                                                                                                                       >  2:535546/1‑140 (MQ=255)         tGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGttgttg                                                                                                                   <  1:88660/140‑1 (MQ=255)                   gtggGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCActc                                                                                                         <  1:85697/140‑1 (MQ=255)                     gggAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCActcct                                                                                                       <  2:750794/140‑1 (MQ=255)                             agCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGTGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGaa                                                                                               >  1:791565/1‑140 (MQ=255)                             agCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGaa                                                                                               >  2:661390/1‑140 (MQ=255)                             agCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGCGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGATGAATTGGACTCCTCCGTGGaa                                                                                               >  2:565330/1‑140 (MQ=255)                              gCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGAAt                                                                                              <  1:246856/140‑1 (MQ=255)                                 aGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACg                                                                                           >  2:289576/1‑140 (MQ=255)                                      cTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAGCAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTc                                                                                      <  2:656698/140‑1 (MQ=255)                                                      gCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTc                                                                      >  1:801683/1‑140 (MQ=255)                                                             tGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCggg                                                               <  1:289576/140‑1 (MQ=255)                                                                  ttCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTcc                                                          <  1:364875/140‑1 (MQ=255)                                                                                  tggtgCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGttt                                          >  1:198190/1‑140 (MQ=255)                                                                                    gtgCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCgg                                                <  1:796269/132‑1 (MQ=255)                                                                                    gtgCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCgg                                                >  2:796269/1‑132 (MQ=255)                                                                                    gtgCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGc                                        >  1:598057/1‑140 (MQ=255)                                                                                     tgCAGGCGGACCGTATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGcc                                       >  2:59378/1‑140 (MQ=255)                                                                                       cAGGCGGACCGGATTTGGTGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCgg                                     <  2:791565/140‑1 (MQ=255)                                                                                                        gCGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGaa                                   >  2:134019/1‑125 (MQ=255)                                                                                                        gCGGTGGTCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGaa                                   <  1:134019/125‑1 (MQ=255)                                                                                                            tggtCGCGGCGCATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGgc                >  1:698140/1‑140 (MQ=255)                                                                                                                    gcgcATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGaa        <  2:801683/140‑1 (MQ=255)                                                                                                                     cgcATTGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAAc       <  2:481253/140‑1 (MQ=255)                                                                                                                          tGCGGGTTGAGCG‑AAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCgcgc  >  2:100120/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                AGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCATTGCGGGTTGAGCGAAAAACAGGTTGTTGAATTGCACTCCTCCGTGGAATACGTGGTCTGGTTTTTAGGTTTTCAACCGGGCTTCCCGTATCTCGGGAGTTTGCCGGAACAACTACACACGCCACGGCGCGCTGAACCGCGC  >  NC_000913/743796‑744057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑