Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,449,064	(T)7→6	intergenic (+46/+12)	feaB → / ← tynA	phenylacetaldehyde dehydrogenase/copper‑containing amine oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,449,058	0	T	.	100.0%	143.7 / NA	35	intergenic (+40/+18)	feaB/tynA	phenylacetaldehyde dehydrogenase/copper‑containing amine oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (19/16);  total (19/16)

TGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCG  >  NC_000913/1448933‑1449184                                                                                                                              |                                                                                                                               tGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAAc                                                                                                                 >  1:468174/1‑140 (MQ=255)     gCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATc                                                                                                             >  2:660665/1‑140 (MQ=255)        gTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACt                                                                                                          <  2:468174/140‑1 (MQ=255)         tCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGaa                                                                                                                   <  1:217434/130‑1 (MQ=255)         tCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGaa                                                                                                                   >  2:217434/1‑130 (MQ=255)           aGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATc                                                                                                             >  1:154807/1‑134 (MQ=255)           aGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATc                                                                                                             <  2:154807/134‑1 (MQ=255)                   gCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTttcttc                                                                                               >  2:703464/1‑140 (MQ=255)                   gCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTttcttc                                                                                               >  1:360187/1‑140 (MQ=255)                     cGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAg                                                                                             <  1:87512/140‑1 (MQ=255)                           tttGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGcccc                                                                                       >  1:638586/1‑140 (MQ=255)                                 cccGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGt                                                                                 >  1:18640/1‑140 (MQ=255)                                                 gTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAaag                                                                 >  1:745696/1‑140 (MQ=255)                                                 gTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAaag                                                                 <  1:310643/140‑1 (MQ=255)                                                  ttGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAaaga                                                                >  2:747669/1‑140 (MQ=255)                                                   tGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAaagaa                                                               >  2:280558/1‑140 (MQ=255)                                                        tgAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTcc                                                          >  1:358606/1‑140 (MQ=255)                                                        tgAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTcc                                                          <  1:197992/140‑1 (MQ=255)                                                             cTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGt                                                     <  1:752915/140‑1 (MQ=255)                                                                    ggTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTcagca                                              <  1:630158/140‑1 (MQ=255)                                                                    ggTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTcagca                                              <  2:126718/140‑1 (MQ=255)                                                                      tgtgtgtACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCaga                                            >  2:192863/1‑140 (MQ=255)                                                                         gtgtACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTa                                         <  2:638586/140‑1 (MQ=255)                                                                            tACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGt                                      >  2:281473/1‑140 (MQ=255)                                                                                  aTTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCAt                                >  2:759582/1‑140 (MQ=255)                                                                                     aaTCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATtcg                             <  1:703464/140‑1 (MQ=255)                                                                                     aaTCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATtcg                             <  2:358606/140‑1 (MQ=255)                                                                                     aaTCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATtcg                             <  1:660665/140‑1 (MQ=255)                                                                                           gTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAATAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGc                       >  2:769669/1‑140 (MQ=255)                                                                                                 tCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATtcg                             >  2:525344/1‑128 (MQ=255)                                                                                                 tCATAAGTAAAAAACGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATtcg                             <  1:525344/128‑1 (MQ=255)                                                                                                      aGTAAAAAACGACACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCa            >  2:281092/1‑140 (MQ=255)                                                                                                             aaCGGCAGCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCg     >  2:798570/1‑140 (MQ=255)                                                                                                             aaCGGCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCg     >  2:530296/1‑140 (MQ=255)                                                                                                                ggCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGgcg  <  1:281473/140‑1 (MQ=255)                                                                                                                ggCACCTGGTGCCG‑TTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGgcg  <  2:745696/140‑1 (MQ=255)                                                                                                                              |                                                                                                                               TGAAGCAGTCAGGAACGGGCCGTGATTTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAATCTGGTTCGCTCATAAGTAAAAAACGGCACCTGGTGCCGTTTTTTTGTCTGAAACAATCACTTATCTTTCTTCAGCGCCCCTAGCGTTGGCGTTTCGTCAAAGAAGTTCCATGGTTTCAGCAGAGTATGTACCCATTCGGTCGGCATAATCGGCCACTCTTCGGCG  >  NC_000913/1448933‑1449184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑