| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,871,832 | (G)6→7 | coding (1448/1476 nt) | cdgI → | putative c‑di‑GMP binding protein CdgI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,871,826 | 1 | . | G | 94.1% | 108.1 / 0.4 | 34 | coding (1442/1476 nt) | cdgI | putative c‑di‑GMP binding protein CdgI |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (13/19); total (14/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01 | |||||||||||
CTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAATTTCCCCTCCCCAGCGGCGCAGAGA > NC_000913/1871687‑1871954 | cttaacGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCCGCCGTATTGGCCCGGTTTTTTCAACGGGCGACACGCGTAATCTCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGCAAAGCGa < 2:56778/135‑1 (MQ=255)cTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGa < 1:245193/140‑1 (MQ=255)cTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGa < 1:49976/140‑1 (MQ=255) gACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑gg < 1:128199/140‑1 (MQ=255) gACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑gg > 2:72365/1‑140 (MQ=255) ccGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑gggg < 2:229994/140‑1 (MQ=255) cGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑ggggg > 1:392428/1‑140 (MQ=255) ttAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑gggggggcaa < 1:544699/140‑5 (MQ=255) ttAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑gg > 2:431575/1‑132 (MQ=255) ttAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑gg > 2:496881/1‑132 (MQ=255) ttAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑gg < 1:431575/132‑1 (MQ=255) ttAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑gg < 1:496881/132‑1 (MQ=255) aGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGa < 2:164901/140‑1 (MQ=255) gatgTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATtc > 1:668975/1‑140 (MQ=255) ttCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGGGCAACAAAGTGATTATtcatc > 1:115763/1‑140 (MQ=255) ccTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCata > 2:238014/1‑140 (MQ=255) aaCAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAGGGGGGGGCAACAAAGTGATTATTCATCATAttt < 2:115763/140‑1 (MQ=255) aaCAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTa > 2:475054/1‑140 (MQ=255) ccATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCaaaa < 2:668975/140‑1 (MQ=255) cACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCa < 1:475054/140‑1 (MQ=255) ggTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGc > 2:123834/1‑140 (MQ=255) gTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCt > 2:786479/1‑140 (MQ=255) aaCGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGt < 1:123834/140‑1 (MQ=255) gTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCtt < 2:138176/140‑1 (MQ=255) gTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCtt < 1:72365/140‑1 (MQ=255) acGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCa < 1:786479/140‑1 (MQ=255) gcgTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATa > 2:377064/1‑140 (MQ=255) gTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACa < 2:166358/140‑1 (MQ=255) gTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACa > 2:694879/1‑140 (MQ=255) tAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCg < 1:746797/124‑1 (MQ=255) tAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCg > 2:746797/1‑124 (MQ=255) tcGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATttatta > 2:592882/1‑140 (MQ=255) cGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCttt < 2:28698/123‑1 (MQ=255) cGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCttt > 1:28698/1‑123 (MQ=255) cGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTtattac > 1:162354/1‑140 (MQ=255) ttGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTtattactatt > 2:796109/1‑140 (MQ=255) gCAGATAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAATTtc < 2:261792/140‑1 (MQ=255) aTAAAGCCTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAATTtcccct > 2:210622/1‑140 (MQ=255) ccTTACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAATTTCCCCTCCCCAg < 1:231937/140‑1 (MQ=255) ttACGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAATTTCCCCTCCCCAgcg < 1:210622/140‑1 (MQ=255) aCGCGAAGCGAAAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAATTTCCCCTCCCCAgcggc < 1:694879/140‑1 (MQ=255) aCGCGAAGCGAAAAGCGA‑‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAATTTCCCCTCCCCAgcggcg < 1:796109/140‑1 (MQ=255) agcgaaAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAATTTCCCCTCCCCAGCGGCGCagag < 1:238014/140‑1 (MQ=255) gcgaaAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAATTTCCCCTCCCCAGCGGCGCAgaga < 1:377064/140‑1 (MQ=255) gcgaaAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAATTTCCCCTCCCCAGCGGCGCAgaga < 2:340620/140‑1 (MQ=255) gcgaaAAGCGAG‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAATTTCCCCTCCCCAGCGGCGCAgaga < 2:162354/140‑1 (MQ=255) | CTGACCGGCTTTAGTAACAGATATGATGTTCCTGAACAAATGACCATCAGTATTGGCACGGTTTTTTCAACGGGTGACACGCGTAATATCTCGCTTGTCATGACGGAAGCAGATAAAGCCTTACGCGAAGCGAAAAGCGA‑‑GGGGGGCAACAAAGTGATTATTCATCATATTTAAGTGCAAAAATATTCAGAGCCATGCTTTTTGCGTGGCTTTTGCATACAATTTATTACTATTACCCCTAAATTTCCCCTCCCCAGCGGCGCAGAGA > NC_000913/1871687‑1871954 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |