Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	303,093	(G)6→7	coding (103/615 nt)	yagU →	inner membrane protein that contributes to acid resistance

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	303,087	1	.	G	100.0%	303.4 / NA	86	W33G (TGG→GGG)	yagU	inner membrane protein that contributes to acid resistance
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (43/43);  total (43/43)

GAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGT‑GGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTT  >  NC_000913/302956‑303219                                                                                                                                     |                                                                                                                                     gagGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGT‑gggggggc                                                                                                                              >  2:12441/1‑138 (MQ=255)  agGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGT‑gggggggct                                                                                                                             >  2:566745/1‑137 (MQ=255)   ggTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGT‑gggggggctg                                                                                                                            <  1:314842/140‑5 (MQ=255)    gtgtATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGT‑gggggggctga                                                                                                                           >  1:364243/1‑135 (MQ=255)      gtATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGT‑ggggg                                                                                                                                 >  2:141737/1‑132 (MQ=255)      gtATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGT‑ggggg                                                                                                                                 <  1:141737/132‑1 (MQ=255)       tataACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGt                                                                                                                         >  1:436450/1‑140 (MQ=255)            cATAATGGTACAAGCAGGGTAGATATGAATATATCTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCAt                                                                                                                    <  2:43631/140‑1 (MQ=255)              tAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTg                                                                                                                  >  2:684404/1‑140 (MQ=255)              tAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTg                                                                                                                  >  1:752029/1‑140 (MQ=255)              tAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTAGTGAAGTGGGGGGGCTGAAGTTCCATTg                                                                                                                  >  1:133421/1‑140 (MQ=255)               aaTGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTgc                                                                                                                 >  1:215206/1‑140 (MQ=255)                aTGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTgcc                                                                                                                <  1:12441/140‑1 (MQ=255)                 tGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTgccg                                                                                                               <  2:215206/140‑1 (MQ=255)                 tGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTgccg                                                                                                               >  1:400153/1‑140 (MQ=255)                 tGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTgccg                                                                                                               <  1:529374/140‑1 (MQ=255)                 tGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTgccg                                                                                                               >  2:358864/1‑140 (MQ=255)                 tGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTgcc                                                                                                                >  2:103342/1‑139 (MQ=255)                 tGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTgcc                                                                                                                <  1:103342/139‑1 (MQ=255)                    tACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCa                                                                                                            <  1:64067/140‑1 (MQ=255)                    tACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCa                                                                                                            >  1:692498/1‑140 (MQ=255)                     aCAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCAc                                                                                                           <  2:691711/140‑1 (MQ=255)                      cAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACg                                                                                                          >  2:120176/1‑140 (MQ=255)                      cAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACg                                                                                                          <  1:725216/140‑1 (MQ=255)                      cAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACg                                                                                                          >  1:748216/1‑140 (MQ=255)                      cAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACg                                                                                                          >  2:689075/1‑140 (MQ=255)                         gCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAg                                                                                                       <  1:330614/140‑1 (MQ=255)                           aGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGcc                                                                                                     <  2:253538/140‑1 (MQ=255)                           aGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGcc                                                                                                     >  2:397093/1‑140 (MQ=255)                           aGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGcc                                                                                                     <  1:689075/140‑1 (MQ=255)                           aGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGcc                                                                                                     <  1:358864/140‑1 (MQ=255)                             ggTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGccc                                                                                                    >  1:102524/1‑139 (MQ=255)                             ggTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGccc                                                                                                    <  2:102524/139‑1 (MQ=255)                             ggTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCg                                                                                                   <  1:612501/140‑1 (MQ=255)                              gTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCgg                                                                                                  <  2:400153/140‑1 (MQ=255)                              gTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCgg                                                                                                  <  1:599967/140‑1 (MQ=255)                              gTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCgg                                                                                                  <  1:120176/140‑1 (MQ=255)                              gTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCgg                                                                                                  >  2:4921/1‑140 (MQ=255)                              gTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCgg                                                                                                  <  1:4921/140‑1 (MQ=255)                                 gATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTgg                                                                                               <  2:748216/140‑1 (MQ=255)                                   tatGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGat                                                                                             >  1:628932/1‑140 (MQ=255)                                       aaTATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGcc                                                                                                     <  2:193376/128‑1 (MQ=255)                                       aaTATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGat                                                                                             <  1:353723/136‑1 (MQ=255)                                       aaTATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGat                                                                                             >  2:353723/1‑136 (MQ=255)                                       aaTATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGat                                                                                             <  2:342319/136‑1 (MQ=255)                                       aaTATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGat                                                                                             >  1:342319/1‑136 (MQ=255)                                       aaTATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGt                                                                                         >  1:715331/1‑140 (MQ=255)                                       aaTATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGt                                                                                         >  1:10870/1‑140 (MQ=255)                                       aaTATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGACACGTAGcc                                                                                                     >  1:193376/1‑128 (MQ=255)                                         tatatTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGttt                                                                                       >  2:470566/1‑140 (MQ=255)                                            atTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAAt                                                                                    >  1:664943/1‑140 (MQ=255)                                             tttGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATg                                                                                   >  1:35507/1‑140 (MQ=255)                                             tttGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATg                                                                                   >  2:550096/1‑140 (MQ=255)                                              ttGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTAGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGc                                                                                  <  2:133421/140‑1 (MQ=255)                                               tGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCa                                                                                 <  1:397093/140‑1 (MQ=255)                                                 aaCAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGc                                                                               >  1:376794/1‑140 (MQ=255)                                                 aaCAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGc                                                                               >  2:434713/1‑140 (MQ=255)                                                    aaaCTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTCTAATTCAGCgtg                                                                            <  2:364243/140‑1 (MQ=255)                                                      aCTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGtgtg                                                                          >  2:182760/1‑140 (MQ=255)                                                       cTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTgg                                                                         >  2:731822/1‑140 (MQ=255)                                                           aCCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCg                                                                     >  2:543594/1‑140 (MQ=255)                                                            ccGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCgg                                                                    <  2:375191/140‑1 (MQ=255)                                                            ccGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCgg                                                                    >  1:49429/1‑140 (MQ=255)                                                            ccGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCgg                                                                    >  2:507409/1‑140 (MQ=255)                                                            ccGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCgg                                                                    <  1:507409/140‑1 (MQ=255)                                                               aaCCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAAt                                                                 >  1:287697/1‑140 (MQ=255)                                                                aCCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATc                                                                >  1:91179/1‑140 (MQ=255)                                                                 ccGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCa                                                               <  1:152183/140‑1 (MQ=255)                                                                  cGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCAt                                                              >  2:708587/1‑140 (MQ=255)                                                                         gTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAg                                                       <  2:287697/140‑1 (MQ=255)                                                                         gTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAg                                                       <  1:708587/140‑1 (MQ=255)                                                                               gTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGc                                                 <  2:752029/140‑1 (MQ=255)                                                                               gTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGc                                                 <  2:436450/140‑1 (MQ=255)                                                                                   tgctgcATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGc                                             >  2:664297/1‑140 (MQ=255)                                                                                     ctgcATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCa                                           <  2:794360/140‑1 (MQ=255)                                                                                     ctgcATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCa                                           >  1:245498/1‑140 (MQ=255)                                                                                            cattGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAattgat                                    >  1:20058/1‑140 (MQ=255)                                                                                              ttGGACTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAattgattg                                  >  1:624255/1‑140 (MQ=255)                                                                                                gggCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCt                                <  1:543594/140‑1 (MQ=255)                                                                                                 ggCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTc                               <  1:205417/140‑1 (MQ=255)                                                                                                   cTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTcgc                             <  2:10870/140‑1 (MQ=255)                                                                                                      aTTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTa                          >  2:175996/1‑140 (MQ=255)                                                                                                      aTTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTa                          >  2:731387/1‑140 (MQ=255)                                                                                                       ttGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTaa                         >  1:577169/1‑140 (MQ=255)                                                                                                        tGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAAt                        <  2:697776/140‑1 (MQ=255)                                                                                                        tGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAAt                        <  2:715331/140‑1 (MQ=255)                                                                                                            ggCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTtc                    <  2:628170/140‑1 (MQ=255)                                                                                                            ggCGTTGTTTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTtc                    >  1:628170/1‑140 (MQ=255)                                                                                                                 tgttTCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAAt               <  2:49429/140‑1 (MQ=255)                                                                                                                 tgttTCCGCACTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAAt               <  2:719454/140‑1 (MQ=255)                                                                                                                   tttCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATcc             <  2:300721/140‑1 (MQ=255)                                                                                                                    ttCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCa            <  1:470566/140‑1 (MQ=255)                                                                                                                    ttCCGCATTCGTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCa            <  2:628932/140‑1 (MQ=255)                                                                                                                              gTGAAGTGGGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATAttt  <  1:550096/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                     GAGGTGTATAACATAATGGTACAAGCAGGGTAGATATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATTGGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGT‑GGGGGGCTGAAGTTCCATTGCCGCCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCTGCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTT  >  NC_000913/302956‑303219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑